Neuroprotective effects of gastrodin in both in vivo and in vitro models of Parkinson's disease: Mechanisms from the perspective of oxidative stress, ferroptosis, and cellular senescence
Aims: To investigate the effect of GAS in PD models by regulating ferroptosis and cell senescence. Methods: MPTP or MPP+ was used to establish PD models in vivo or in vitro, and the effect of GAS was detected by behavioral analysis, immunohistochemistry and cell viability. At the same time, transmis...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464625000246 |
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| author | Shanshan Li Meng Wang Buyun Jia Baikun Li Hui Cheng Shasha Tian Qinglin Li |
| author_facet | Shanshan Li Meng Wang Buyun Jia Baikun Li Hui Cheng Shasha Tian Qinglin Li |
| author_sort | Shanshan Li |
| collection | DOAJ |
| description | Aims: To investigate the effect of GAS in PD models by regulating ferroptosis and cell senescence. Methods: MPTP or MPP+ was used to establish PD models in vivo or in vitro, and the effect of GAS was detected by behavioral analysis, immunohistochemistry and cell viability. At the same time, transmission electron microscopy, biochemical kits, Western blotting, immunofluorescence, and molecular docking were used. Results: We found that gastrodin could inhibit cell senescence in PD models. In addition, GAS was found to inhibit ferroptosis, regulate oxidative stress level, and level of ACSL4, FTH1, SLC7A11, GPX4. We also found that Ferrostatin-1 and GAS could inhibit cell senescence. The results of molecular docking and immunofluorescence showed that p53 may be an important target of GAS. After treatment with Pifithrin-α, the protective effect of GAS was enhanced. Conclusion: GAS inhibits cellular senescence in PD models and treats PD through a novel mechanism related to ferroptosis. |
| format | Article |
| id | doaj-art-5815c2d2940145258c46e0a688187d99 |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-5815c2d2940145258c46e0a688187d992025-02-09T04:59:57ZengElsevierJournal of Functional Foods1756-46462025-02-01125106682Neuroprotective effects of gastrodin in both in vivo and in vitro models of Parkinson's disease: Mechanisms from the perspective of oxidative stress, ferroptosis, and cellular senescenceShanshan Li0Meng Wang1Buyun Jia2Baikun Li3Hui Cheng4Shasha Tian5Qinglin Li6Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, ChinaLaboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, ChinaLaboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, ChinaLaboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, ChinaLaboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, ChinaSchool of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, ChinaLaboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, China; Corresponding author at: Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, China.Aims: To investigate the effect of GAS in PD models by regulating ferroptosis and cell senescence. Methods: MPTP or MPP+ was used to establish PD models in vivo or in vitro, and the effect of GAS was detected by behavioral analysis, immunohistochemistry and cell viability. At the same time, transmission electron microscopy, biochemical kits, Western blotting, immunofluorescence, and molecular docking were used. Results: We found that gastrodin could inhibit cell senescence in PD models. In addition, GAS was found to inhibit ferroptosis, regulate oxidative stress level, and level of ACSL4, FTH1, SLC7A11, GPX4. We also found that Ferrostatin-1 and GAS could inhibit cell senescence. The results of molecular docking and immunofluorescence showed that p53 may be an important target of GAS. After treatment with Pifithrin-α, the protective effect of GAS was enhanced. Conclusion: GAS inhibits cellular senescence in PD models and treats PD through a novel mechanism related to ferroptosis.http://www.sciencedirect.com/science/article/pii/S1756464625000246Parkinson's diseaseFerroptosisCell senescenceGastrodin |
| spellingShingle | Shanshan Li Meng Wang Buyun Jia Baikun Li Hui Cheng Shasha Tian Qinglin Li Neuroprotective effects of gastrodin in both in vivo and in vitro models of Parkinson's disease: Mechanisms from the perspective of oxidative stress, ferroptosis, and cellular senescence Journal of Functional Foods Parkinson's disease Ferroptosis Cell senescence Gastrodin |
| title | Neuroprotective effects of gastrodin in both in vivo and in vitro models of Parkinson's disease: Mechanisms from the perspective of oxidative stress, ferroptosis, and cellular senescence |
| title_full | Neuroprotective effects of gastrodin in both in vivo and in vitro models of Parkinson's disease: Mechanisms from the perspective of oxidative stress, ferroptosis, and cellular senescence |
| title_fullStr | Neuroprotective effects of gastrodin in both in vivo and in vitro models of Parkinson's disease: Mechanisms from the perspective of oxidative stress, ferroptosis, and cellular senescence |
| title_full_unstemmed | Neuroprotective effects of gastrodin in both in vivo and in vitro models of Parkinson's disease: Mechanisms from the perspective of oxidative stress, ferroptosis, and cellular senescence |
| title_short | Neuroprotective effects of gastrodin in both in vivo and in vitro models of Parkinson's disease: Mechanisms from the perspective of oxidative stress, ferroptosis, and cellular senescence |
| title_sort | neuroprotective effects of gastrodin in both in vivo and in vitro models of parkinson s disease mechanisms from the perspective of oxidative stress ferroptosis and cellular senescence |
| topic | Parkinson's disease Ferroptosis Cell senescence Gastrodin |
| url | http://www.sciencedirect.com/science/article/pii/S1756464625000246 |
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