DNA metabarcoding of spider egg sacs uncovers novel insights into host parasitoid complexes and trophic networks
Abstract DNA metabarcoding is a rapid tool for sequencing a multitude of samples at once, to identify associated organisms. This pioneering study, using DNA metabarcoding of spider egg sacs, establishes a framework for investigating host-parasitoid associations. While traditional rearing methods ide...
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Nature Portfolio
2025-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-97799-2 |
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| author | Rupam Debnath K. Rajmohana V. Sushama K. P. Dinesh |
| author_facet | Rupam Debnath K. Rajmohana V. Sushama K. P. Dinesh |
| author_sort | Rupam Debnath |
| collection | DOAJ |
| description | Abstract DNA metabarcoding is a rapid tool for sequencing a multitude of samples at once, to identify associated organisms. This pioneering study, using DNA metabarcoding of spider egg sacs, establishes a framework for investigating host-parasitoid associations. While traditional rearing methods identified ten arthropod taxa, DNA metabarcoding aided in resolving the identity of 28 arthropod molecular operational taxonomic units (MOTUs), 14 of which represented host-parasitoid associations. The study provides the first documented occurrence of the genus Aphanogmus Thomson (Hymenoptera: Ceraphronoidea: Ceraphronidae) within spider egg sacs. This research also emphasizes the importance of studying lesser-known associations to gain insights into the complexity of ecological networks and trophic interactions within arthropod communities. However, a well-curated DNA reference library and a comprehensive understanding of the functional roles of the studied taxa are essential prerequisites for accurately characterizing these complex trophic networks. |
| format | Article |
| id | doaj-art-57fe5e53752a4aae9604f54e19190a93 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-57fe5e53752a4aae9604f54e19190a932025-08-20T02:24:29ZengNature PortfolioScientific Reports2045-23222025-04-0115111210.1038/s41598-025-97799-2DNA metabarcoding of spider egg sacs uncovers novel insights into host parasitoid complexes and trophic networksRupam Debnath0K. Rajmohana1V. Sushama2K. P. Dinesh3Zoological Survey of IndiaZoological Survey of IndiaZoological Survey of IndiaZoological Survey of India, Western Regional CenterAbstract DNA metabarcoding is a rapid tool for sequencing a multitude of samples at once, to identify associated organisms. This pioneering study, using DNA metabarcoding of spider egg sacs, establishes a framework for investigating host-parasitoid associations. While traditional rearing methods identified ten arthropod taxa, DNA metabarcoding aided in resolving the identity of 28 arthropod molecular operational taxonomic units (MOTUs), 14 of which represented host-parasitoid associations. The study provides the first documented occurrence of the genus Aphanogmus Thomson (Hymenoptera: Ceraphronoidea: Ceraphronidae) within spider egg sacs. This research also emphasizes the importance of studying lesser-known associations to gain insights into the complexity of ecological networks and trophic interactions within arthropod communities. However, a well-curated DNA reference library and a comprehensive understanding of the functional roles of the studied taxa are essential prerequisites for accurately characterizing these complex trophic networks.https://doi.org/10.1038/s41598-025-97799-2ArachnidaBaeiniHigh-throughput sequencingIndiaScelionidaeTrophic interactions |
| spellingShingle | Rupam Debnath K. Rajmohana V. Sushama K. P. Dinesh DNA metabarcoding of spider egg sacs uncovers novel insights into host parasitoid complexes and trophic networks Scientific Reports Arachnida Baeini High-throughput sequencing India Scelionidae Trophic interactions |
| title | DNA metabarcoding of spider egg sacs uncovers novel insights into host parasitoid complexes and trophic networks |
| title_full | DNA metabarcoding of spider egg sacs uncovers novel insights into host parasitoid complexes and trophic networks |
| title_fullStr | DNA metabarcoding of spider egg sacs uncovers novel insights into host parasitoid complexes and trophic networks |
| title_full_unstemmed | DNA metabarcoding of spider egg sacs uncovers novel insights into host parasitoid complexes and trophic networks |
| title_short | DNA metabarcoding of spider egg sacs uncovers novel insights into host parasitoid complexes and trophic networks |
| title_sort | dna metabarcoding of spider egg sacs uncovers novel insights into host parasitoid complexes and trophic networks |
| topic | Arachnida Baeini High-throughput sequencing India Scelionidae Trophic interactions |
| url | https://doi.org/10.1038/s41598-025-97799-2 |
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