Nasal polyposis: A semiquantitative morphometric histopathological study

Background/Aim. Nasal polyps are inflammatory hyper-trophic proliferations of the sinonasal mucosa composed of both epithelial and stromal elements. The aim of this study was to determine histopathological hallmarks of nasal polyposis via semiquantitative morphometric study. Methods. The study compr...

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Main Authors: Trivić Aleksandar, Tomanović Nada, Krejović-Trivić Sanja, Milovanović Jovica, Boričić Ivan, Jotić Ana, Folić Miljan, Ćolović-Čalovski Ivana, Miković Nikola, Tatić Zoran
Format: Article
Language:English
Published: Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade 2020-01-01
Series:Vojnosanitetski Pregled
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Online Access:http://www.doiserbia.nb.rs/img/doi/0042-8450/2020/0042-84501800139T.pdf
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author Trivić Aleksandar
Tomanović Nada
Krejović-Trivić Sanja
Milovanović Jovica
Boričić Ivan
Jotić Ana
Folić Miljan
Ćolović-Čalovski Ivana
Miković Nikola
Tatić Zoran
author_facet Trivić Aleksandar
Tomanović Nada
Krejović-Trivić Sanja
Milovanović Jovica
Boričić Ivan
Jotić Ana
Folić Miljan
Ćolović-Čalovski Ivana
Miković Nikola
Tatić Zoran
author_sort Trivić Aleksandar
collection DOAJ
description Background/Aim. Nasal polyps are inflammatory hyper-trophic proliferations of the sinonasal mucosa composed of both epithelial and stromal elements. The aim of this study was to determine histopathological hallmarks of nasal polyposis via semiquantitative morphometric study. Methods. The study comprised 77 patients with chronic rhinosinusitis and nasal polyposis (CRSwNP) that underwent functional endoscopic sinonasal surgery performed by the same surgeon. The control group consisted of 9 different nasal mucosal samples that were taken from patients without CRSwNP that underwent functional and esthetic surgery. Morphometric analysis included gradation of tissue edema within polyps, thickening of epithelial basal membrane, degree of inflammation, presence/absence of metaplasia with-in epithelium, degree of fibrosis within polyps, and percentage of inflammatory cells within inflammatory infiltrate (lymphocytes, macrophages, plasma cells, neutrophils and eosinophils). Results. As expected, samples from the study group showed significantly higher degree of inflammation than samples from the control group (χ2 = 35.89, with p < 0.01). Degree of fibrosis in nasal polyposis was in positive correlation with duration of symptoms (r = 0.25, p < 0.05) and with percentage of macrophages in inflammatory infiltrate (r = 0.26, p < 0.05). Patients with nasal polyposis had significantly lower number of lymphocytes (r = -7.66, p < 0.01), but significantly higher number of eosinophils (r = 3.84, p < 0.01), macrophages (r = 3.34, p < 0.01) and plasma cells (r = 3.14, p < 0.01) than controls (p < 0.01). Conclusion. Tissue samples from patients with nasal polyposis show significant changes that reflect in various degrees of inflammation, fibrosis and basement membrane thickening which may contribute to more difficult surgical management and perioperative complications such as bleeding.
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spelling doaj-art-57f85564506344efb658108951da4c4d2025-08-20T02:05:48ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202020-01-0177878478810.2298/VSP171228139T0042-84501800139TNasal polyposis: A semiquantitative morphometric histopathological studyTrivić Aleksandar0Tomanović Nada1Krejović-Trivić Sanja2Milovanović Jovica3Boričić Ivan4Jotić Ana5Folić Miljan6Ćolović-Čalovski Ivana7Miković Nikola8Tatić Zoran9Clinical Centre of Serbia, Clinic for Otorhinolaryngology and Maxillofacial Surgery, Belgrade, Serbia + University of Belgrade, Faculty of Medicine, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, Belgrade, Serbia + University of Belgrade, Faculty of Medicine, Institute of Pathology, Belgrade, SerbiaClinical Centre of Serbia, Clinic for Otorhinolaryngology and Maxillofacial Surgery, Belgrade, Serbia + University of Belgrade, Faculty of Medicine, Belgrade, SerbiaClinical Centre of Serbia, Clinic for Otorhinolaryngology and Maxillofacial Surgery, Belgrade, Serbia + University of Belgrade, Faculty of Medicine, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, Belgrade, Serbia + University of Belgrade, Faculty of Medicine, Institute of Pathology, Belgrade, SerbiaClinical Centre of Serbia, Clinic for Otorhinolaryngology and Maxillofacial Surgery, Belgrade, Serbia + University of Belgrade, Faculty of Medicine, Belgrade, SerbiaClinical Centre of Serbia, Clinic for Otorhinolaryngology and Maxillofacial Surgery, Belgrade, Serbia + University of Belgrade, Faculty of Medicine, Belgrade, SerbiaUniversity of Belgrade, Faculty of Medicine, Belgrade, Serbia + University of Belgrade, Faculty of Medicine, Institute of Microbiology and Immunology, Belgrade, SerbiaUniversity of Belgrade, Faculty of Dentistry, Clinic for Maxillofacial Surgery, Belgrade, SerbiaMilitary Medical Academy, Dental Clinic, Belgrade, Serbia + University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, SerbiaBackground/Aim. Nasal polyps are inflammatory hyper-trophic proliferations of the sinonasal mucosa composed of both epithelial and stromal elements. The aim of this study was to determine histopathological hallmarks of nasal polyposis via semiquantitative morphometric study. Methods. The study comprised 77 patients with chronic rhinosinusitis and nasal polyposis (CRSwNP) that underwent functional endoscopic sinonasal surgery performed by the same surgeon. The control group consisted of 9 different nasal mucosal samples that were taken from patients without CRSwNP that underwent functional and esthetic surgery. Morphometric analysis included gradation of tissue edema within polyps, thickening of epithelial basal membrane, degree of inflammation, presence/absence of metaplasia with-in epithelium, degree of fibrosis within polyps, and percentage of inflammatory cells within inflammatory infiltrate (lymphocytes, macrophages, plasma cells, neutrophils and eosinophils). Results. As expected, samples from the study group showed significantly higher degree of inflammation than samples from the control group (χ2 = 35.89, with p < 0.01). Degree of fibrosis in nasal polyposis was in positive correlation with duration of symptoms (r = 0.25, p < 0.05) and with percentage of macrophages in inflammatory infiltrate (r = 0.26, p < 0.05). Patients with nasal polyposis had significantly lower number of lymphocytes (r = -7.66, p < 0.01), but significantly higher number of eosinophils (r = 3.84, p < 0.01), macrophages (r = 3.34, p < 0.01) and plasma cells (r = 3.14, p < 0.01) than controls (p < 0.01). Conclusion. Tissue samples from patients with nasal polyposis show significant changes that reflect in various degrees of inflammation, fibrosis and basement membrane thickening which may contribute to more difficult surgical management and perioperative complications such as bleeding.http://www.doiserbia.nb.rs/img/doi/0042-8450/2020/0042-84501800139T.pdfnasal polypsotorhinolaryngologic surgical procedurespostoperative complicationshistology
spellingShingle Trivić Aleksandar
Tomanović Nada
Krejović-Trivić Sanja
Milovanović Jovica
Boričić Ivan
Jotić Ana
Folić Miljan
Ćolović-Čalovski Ivana
Miković Nikola
Tatić Zoran
Nasal polyposis: A semiquantitative morphometric histopathological study
Vojnosanitetski Pregled
nasal polyps
otorhinolaryngologic surgical procedures
postoperative complications
histology
title Nasal polyposis: A semiquantitative morphometric histopathological study
title_full Nasal polyposis: A semiquantitative morphometric histopathological study
title_fullStr Nasal polyposis: A semiquantitative morphometric histopathological study
title_full_unstemmed Nasal polyposis: A semiquantitative morphometric histopathological study
title_short Nasal polyposis: A semiquantitative morphometric histopathological study
title_sort nasal polyposis a semiquantitative morphometric histopathological study
topic nasal polyps
otorhinolaryngologic surgical procedures
postoperative complications
histology
url http://www.doiserbia.nb.rs/img/doi/0042-8450/2020/0042-84501800139T.pdf
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