Clinical evaluation of two pathogen enrichment approaches for next-generation sequencing in the diagnosis of lower respiratory tract infections

Clinical evaluation of two pathogen enrichment approaches for next-generation sequencing in the diagnosis of lower respiratory tract infections

ABSTRACT The underdevelopment of microbiological tests has contributed to diagnostic delay and inappropriate use of antibiotics in patients with lower respiratory tract infections, which is ranked as the seventh leading cause of death globally. Next-generation sequencing (NGS) has emerged as a promi...

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Main Authors: Xiao Lei, Xiumei Xu, Chao Liu, Lipeng Zhong, Shupeng Yin, Biaoxian Li, Ling Cao, Zhiting Xie, Jing Li, Xuan Zhang, Yaping Guo, Liang Zhang, Haiyan Lin, Sufeng Zhang, Chengsheng Zhang, Tian Gong
Format: Article
Language:English
Published: American Society for Microbiology 2025-07-01
Series:Microbiology Spectrum
Subjects:
lower respiratory tract infection
targeted next-generation sequencing
multiplex PCR
pathogen enrichment
etiological diagnosis
Online Access:https://journals.asm.org/doi/10.1128/spectrum.00922-25
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