Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential
Allogeneic hematopoietic stem cell transplantation is the main curative therapy for many hematologic malignancies. Its potential relies on graft-versus-tumor effects which associate with graft-versus-host disease. Mesenchymal stromal cells (MSCs) possess immunomodulatory properties that make them at...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.4061/2011/235176 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849415150054932480 |
|---|---|
| author | Jeffery J. Auletta Elizabeth A. Zale Jean F. Welter Luis A. Solchaga |
| author_facet | Jeffery J. Auletta Elizabeth A. Zale Jean F. Welter Luis A. Solchaga |
| author_sort | Jeffery J. Auletta |
| collection | DOAJ |
| description | Allogeneic hematopoietic stem cell transplantation is the main curative therapy for many hematologic malignancies. Its potential relies on graft-versus-tumor effects which associate with graft-versus-host disease. Mesenchymal stromal cells (MSCs) possess immunomodulatory properties that make them attractive therapeutic alternatives. We evaluated the in vitro immunosuppressive activity of medium conditioned by human MSCs from 5 donors expanded 13 passages with or without FGF-2. FGF-2 supplementation increased expansion 3,500- and 240,000-fold by passages 7 and 13, respectively. There were no differences in immunosuppressive activity between media conditioned by passage-matched cells expanded under different conditions, but media conditioned by FGF-treated MSCs were superior to population doubling-matched controls. The immunosuppressive activity was maintained in three of the preparations but decreased with expansion in two. The proliferation induced by FGF-2 did not result in loss of immunosuppressive activity. However, because the immunosuppressive activity was not consistently preserved, caution must be exercised to ensure that the activity of the cells is sufficient after extensive expansion. |
| format | Article |
| id | doaj-art-57e3bcb54c1d49308b8a45f5e985f9da |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-57e3bcb54c1d49308b8a45f5e985f9da2025-08-20T03:33:37ZengWileyStem Cells International1687-966X1687-96782011-01-01201110.4061/2011/235176235176Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive PotentialJeffery J. Auletta0Elizabeth A. Zale1Jean F. Welter2Luis A. Solchaga3Divisions of Pediatric Hematology/Oncology and Pediatric Infectious Diseases, Department of Pediatrics, University Hospitals Case Medical Center, Cleveland, OH 44106, USAHathaway Brown School, 19600 North Park Boulevard, Shaker Heights, OH 44122, USASkeletal Research Center, Department of Biology, Case Western Reserve University, Cleveland, OH 44106-7080, USADivision of Hematology and Oncology, Department of General Medical Sciences, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USAAllogeneic hematopoietic stem cell transplantation is the main curative therapy for many hematologic malignancies. Its potential relies on graft-versus-tumor effects which associate with graft-versus-host disease. Mesenchymal stromal cells (MSCs) possess immunomodulatory properties that make them attractive therapeutic alternatives. We evaluated the in vitro immunosuppressive activity of medium conditioned by human MSCs from 5 donors expanded 13 passages with or without FGF-2. FGF-2 supplementation increased expansion 3,500- and 240,000-fold by passages 7 and 13, respectively. There were no differences in immunosuppressive activity between media conditioned by passage-matched cells expanded under different conditions, but media conditioned by FGF-treated MSCs were superior to population doubling-matched controls. The immunosuppressive activity was maintained in three of the preparations but decreased with expansion in two. The proliferation induced by FGF-2 did not result in loss of immunosuppressive activity. However, because the immunosuppressive activity was not consistently preserved, caution must be exercised to ensure that the activity of the cells is sufficient after extensive expansion.http://dx.doi.org/10.4061/2011/235176 |
| spellingShingle | Jeffery J. Auletta Elizabeth A. Zale Jean F. Welter Luis A. Solchaga Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential Stem Cells International |
| title | Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential |
| title_full | Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential |
| title_fullStr | Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential |
| title_full_unstemmed | Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential |
| title_short | Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential |
| title_sort | fibroblast growth factor 2 enhances expansion of human bone marrow derived mesenchymal stromal cells without diminishing their immunosuppressive potential |
| url | http://dx.doi.org/10.4061/2011/235176 |
| work_keys_str_mv | AT jefferyjauletta fibroblastgrowthfactor2enhancesexpansionofhumanbonemarrowderivedmesenchymalstromalcellswithoutdiminishingtheirimmunosuppressivepotential AT elizabethazale fibroblastgrowthfactor2enhancesexpansionofhumanbonemarrowderivedmesenchymalstromalcellswithoutdiminishingtheirimmunosuppressivepotential AT jeanfwelter fibroblastgrowthfactor2enhancesexpansionofhumanbonemarrowderivedmesenchymalstromalcellswithoutdiminishingtheirimmunosuppressivepotential AT luisasolchaga fibroblastgrowthfactor2enhancesexpansionofhumanbonemarrowderivedmesenchymalstromalcellswithoutdiminishingtheirimmunosuppressivepotential |