A snapshot on molecular technologies for diagnosing FAdV infections
Fowl adenoviruses (FAdV) are prevalent in chickens worldwide, responsible for several poultry diseases, including inclusion body hepatitis (IBH), hepatitis-hydropericardium syndrome (HHS), and gizzard erosion (GE), which result in significant economic losses in the poultry industry. Consequently, de...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1558257/full |
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| author | Amina Kardoudi Fellahi Siham Allaoui Abdelmounaaim Kichou Faouzi Ouchhour Ikram Jackson Thomas Benani Abdelouaheb |
| author_facet | Amina Kardoudi Fellahi Siham Allaoui Abdelmounaaim Kichou Faouzi Ouchhour Ikram Jackson Thomas Benani Abdelouaheb |
| author_sort | Amina Kardoudi |
| collection | DOAJ |
| description | Fowl adenoviruses (FAdV) are prevalent in chickens worldwide, responsible for several poultry diseases, including inclusion body hepatitis (IBH), hepatitis-hydropericardium syndrome (HHS), and gizzard erosion (GE), which result in significant economic losses in the poultry industry. Consequently, detection and efficient identification of FAdV serotypes are becoming extremely urgent to monitor outbreaks and develop vaccination strategies. Conventional PCR (cPCR) tests, combined with Restriction Fragment Length Polymorphism (RFLP) or sequencing, were developed for FAdV diagnosis. Although these molecular tests have considerably improved the accuracy of FAdV diagnosis compared with conventional methods, certain drawbacks remain unresolved, including lack of sensitivity and post-PCR analysis. Subsequently, advanced molecular technologies such as real-time PCR (qPCR), Loop Isothermal Amplification (LAMP), Cross-Priming Amplification (CPA), Recombinase Polymerase Amplification (RPA), Digital Droplet Polymerase Chain Reaction (ddPCR), Dot Blot Assay Combined with cPCR, Nanoparticle-Assisted PCR (nano-PCR), PCR-Refractory Quantitative Amplification (ARMS-qPCR), CRISPR/Cas13a Technology, and High-Resolution Melting Curve (HRM), have been developed to improve FAdV diagnosis. |
| format | Article |
| id | doaj-art-57e2ec7bbff846ac8b1d856fa129ed77 |
| institution | OA Journals |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-57e2ec7bbff846ac8b1d856fa129ed772025-08-20T02:28:58ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-04-011210.3389/fvets.2025.15582571558257A snapshot on molecular technologies for diagnosing FAdV infectionsAmina Kardoudi0Fellahi Siham1Allaoui Abdelmounaaim2Kichou Faouzi3Ouchhour Ikram4Jackson Thomas5Benani Abdelouaheb6Department of Veterinary Pathology and Public Health, Agronomic and Veterinary Institute Hassan II, Rabat, MoroccoDepartment of Veterinary Pathology and Public Health, Agronomic and Veterinary Institute Hassan II, Rabat, MoroccoAfrican Genome Center, Mohammed VI Polytechnic University, Ben Guerir, MoroccoDepartment of Veterinary Pathology and Public Health, Agronomic and Veterinary Institute Hassan II, Rabat, MoroccoDepartment of Veterinary Pathology and Public Health, Agronomic and Veterinary Institute Hassan II, Rabat, MoroccoMilken Institute School of Public Health, George Washington University, Washington, DC, United StatesMedical Biology Department, Molecular Biology Laboratory, Pasteur Institute of Morocco, Casablanca, MoroccoFowl adenoviruses (FAdV) are prevalent in chickens worldwide, responsible for several poultry diseases, including inclusion body hepatitis (IBH), hepatitis-hydropericardium syndrome (HHS), and gizzard erosion (GE), which result in significant economic losses in the poultry industry. Consequently, detection and efficient identification of FAdV serotypes are becoming extremely urgent to monitor outbreaks and develop vaccination strategies. Conventional PCR (cPCR) tests, combined with Restriction Fragment Length Polymorphism (RFLP) or sequencing, were developed for FAdV diagnosis. Although these molecular tests have considerably improved the accuracy of FAdV diagnosis compared with conventional methods, certain drawbacks remain unresolved, including lack of sensitivity and post-PCR analysis. Subsequently, advanced molecular technologies such as real-time PCR (qPCR), Loop Isothermal Amplification (LAMP), Cross-Priming Amplification (CPA), Recombinase Polymerase Amplification (RPA), Digital Droplet Polymerase Chain Reaction (ddPCR), Dot Blot Assay Combined with cPCR, Nanoparticle-Assisted PCR (nano-PCR), PCR-Refractory Quantitative Amplification (ARMS-qPCR), CRISPR/Cas13a Technology, and High-Resolution Melting Curve (HRM), have been developed to improve FAdV diagnosis.https://www.frontiersin.org/articles/10.3389/fvets.2025.1558257/fullfowl adenovirusmolecular diagnosispolymerase chain reactionreal-time PCRisothermal amplificationCRISPR/Cas13 |
| spellingShingle | Amina Kardoudi Fellahi Siham Allaoui Abdelmounaaim Kichou Faouzi Ouchhour Ikram Jackson Thomas Benani Abdelouaheb A snapshot on molecular technologies for diagnosing FAdV infections Frontiers in Veterinary Science fowl adenovirus molecular diagnosis polymerase chain reaction real-time PCR isothermal amplification CRISPR/Cas13 |
| title | A snapshot on molecular technologies for diagnosing FAdV infections |
| title_full | A snapshot on molecular technologies for diagnosing FAdV infections |
| title_fullStr | A snapshot on molecular technologies for diagnosing FAdV infections |
| title_full_unstemmed | A snapshot on molecular technologies for diagnosing FAdV infections |
| title_short | A snapshot on molecular technologies for diagnosing FAdV infections |
| title_sort | snapshot on molecular technologies for diagnosing fadv infections |
| topic | fowl adenovirus molecular diagnosis polymerase chain reaction real-time PCR isothermal amplification CRISPR/Cas13 |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1558257/full |
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