Characterization of the volatiles and quality of Ningxiang pork belly and their relationship to changes of microbial community during chilled storage

The correlations between meat quality, dominant microbiota, and key volatile compounds during chill storage were investigated by HS-SPME-GC-MS, Illumina sequencing and correlation analysis. The results indicated that lipid and protein oxidation of pork belly increased significantly on day 4, biogeni...

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Main Authors: Ziyi Hu, Liusha Kuang, Lin Li, Enqi He, Lei Zhou, Aihua Lou, Yan Liu, Jie Luo, Wei Quan, Qingwu Shen
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:CyTA - Journal of Food
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Online Access:https://www.tandfonline.com/doi/10.1080/19476337.2025.2487123
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author Ziyi Hu
Liusha Kuang
Lin Li
Enqi He
Lei Zhou
Aihua Lou
Yan Liu
Jie Luo
Wei Quan
Qingwu Shen
author_facet Ziyi Hu
Liusha Kuang
Lin Li
Enqi He
Lei Zhou
Aihua Lou
Yan Liu
Jie Luo
Wei Quan
Qingwu Shen
author_sort Ziyi Hu
collection DOAJ
description The correlations between meat quality, dominant microbiota, and key volatile compounds during chill storage were investigated by HS-SPME-GC-MS, Illumina sequencing and correlation analysis. The results indicated that lipid and protein oxidation of pork belly increased significantly on day 4, biogenic amine index reaching 344.07 mg/kg on day 6, the total viable count reached 7.98 log10 (CFU/g) at day 8, with Macrococcus caseolyticus (54.60%), unclassified g Acinetobacter (23.70%) and Acinetobacter bereziniae (7.75%) identified as dominant bacteria. HS-SPME-GC-MS identified 66 compounds with tetramethyl-pyrazine, acetoin, 1-hexadecanol, and hexadecanoic acid were found to be related to meat quality and influenced by microbial activity, serving as indicators of pork belly freshness. However, due to the complexity of microbial communities in aquatic products, further research is needed to explore the relationship between changes in microbial communities and the volatile organic compounds (VOCs). These findings provide valuable insight into key factors contributing to spoilage in Ningxiang pork.
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publishDate 2025-12-01
publisher Taylor & Francis Group
record_format Article
series CyTA - Journal of Food
spelling doaj-art-57e20285547f48179a99cb698e8d64e02025-08-20T02:16:21ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452025-12-0123110.1080/19476337.2025.2487123Characterization of the volatiles and quality of Ningxiang pork belly and their relationship to changes of microbial community during chilled storageZiyi Hu0Liusha Kuang1Lin Li2Enqi He3Lei Zhou4Aihua Lou5Yan Liu6Jie Luo7Wei Quan8Qingwu Shen9College of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaCollege of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaCollege of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaCollege of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaCollege of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaCollege of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaCollege of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaCollege of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaCollege of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaCollege of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan, ChinaThe correlations between meat quality, dominant microbiota, and key volatile compounds during chill storage were investigated by HS-SPME-GC-MS, Illumina sequencing and correlation analysis. The results indicated that lipid and protein oxidation of pork belly increased significantly on day 4, biogenic amine index reaching 344.07 mg/kg on day 6, the total viable count reached 7.98 log10 (CFU/g) at day 8, with Macrococcus caseolyticus (54.60%), unclassified g Acinetobacter (23.70%) and Acinetobacter bereziniae (7.75%) identified as dominant bacteria. HS-SPME-GC-MS identified 66 compounds with tetramethyl-pyrazine, acetoin, 1-hexadecanol, and hexadecanoic acid were found to be related to meat quality and influenced by microbial activity, serving as indicators of pork belly freshness. However, due to the complexity of microbial communities in aquatic products, further research is needed to explore the relationship between changes in microbial communities and the volatile organic compounds (VOCs). These findings provide valuable insight into key factors contributing to spoilage in Ningxiang pork.https://www.tandfonline.com/doi/10.1080/19476337.2025.2487123Meat qualityvolatile organic compoundsmicrobiotapork belly
spellingShingle Ziyi Hu
Liusha Kuang
Lin Li
Enqi He
Lei Zhou
Aihua Lou
Yan Liu
Jie Luo
Wei Quan
Qingwu Shen
Characterization of the volatiles and quality of Ningxiang pork belly and their relationship to changes of microbial community during chilled storage
CyTA - Journal of Food
Meat quality
volatile organic compounds
microbiota
pork belly
title Characterization of the volatiles and quality of Ningxiang pork belly and their relationship to changes of microbial community during chilled storage
title_full Characterization of the volatiles and quality of Ningxiang pork belly and their relationship to changes of microbial community during chilled storage
title_fullStr Characterization of the volatiles and quality of Ningxiang pork belly and their relationship to changes of microbial community during chilled storage
title_full_unstemmed Characterization of the volatiles and quality of Ningxiang pork belly and their relationship to changes of microbial community during chilled storage
title_short Characterization of the volatiles and quality of Ningxiang pork belly and their relationship to changes of microbial community during chilled storage
title_sort characterization of the volatiles and quality of ningxiang pork belly and their relationship to changes of microbial community during chilled storage
topic Meat quality
volatile organic compounds
microbiota
pork belly
url https://www.tandfonline.com/doi/10.1080/19476337.2025.2487123
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