Chromosome-level genome assembly and improved annotation of onion genome (Allium cepa L.)

Abstract Onion (Allium cepa L.) is an economically valuable crop, but its large, repeat-enriched genome makes genome assembly difficult and limits molecular breeding and biological studies. Herein, we present a chromosomal-level reference genome assembly of the double-haploid onion line DHW30006, co...

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Main Authors: Heejung Cho, Myunghee Jung, Seung Jae Lee, Ji Yeon Park, Yedomon Ange Bovys Zoclanclounon, Cheol-Woo Kim, JiWon Han, Jung Sun Kim, Do-Sun Kim, Younhee Shin, Yoon-Jung Hwang, Tae-Ho Lee, Si Myung Lee, Sang-Ho Kang, So Youn Won, Jin-Hyun Kim, Hye Yoon Jang, Hye-Eun Lee, Eun Su Lee, Sang-Choon Lee, Hyeonso Ji, Seong-Han Sohn, Byoung Ohg Ahn
Format: Article
Language:English
Published: Nature Portfolio 2025-02-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-04635-3
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author Heejung Cho
Myunghee Jung
Seung Jae Lee
Ji Yeon Park
Yedomon Ange Bovys Zoclanclounon
Cheol-Woo Kim
JiWon Han
Jung Sun Kim
Do-Sun Kim
Younhee Shin
Yoon-Jung Hwang
Tae-Ho Lee
Si Myung Lee
Sang-Ho Kang
So Youn Won
Jin-Hyun Kim
Hye Yoon Jang
Hye-Eun Lee
Eun Su Lee
Sang-Choon Lee
Hyeonso Ji
Seong-Han Sohn
Byoung Ohg Ahn
author_facet Heejung Cho
Myunghee Jung
Seung Jae Lee
Ji Yeon Park
Yedomon Ange Bovys Zoclanclounon
Cheol-Woo Kim
JiWon Han
Jung Sun Kim
Do-Sun Kim
Younhee Shin
Yoon-Jung Hwang
Tae-Ho Lee
Si Myung Lee
Sang-Ho Kang
So Youn Won
Jin-Hyun Kim
Hye Yoon Jang
Hye-Eun Lee
Eun Su Lee
Sang-Choon Lee
Hyeonso Ji
Seong-Han Sohn
Byoung Ohg Ahn
author_sort Heejung Cho
collection DOAJ
description Abstract Onion (Allium cepa L.) is an economically valuable crop, but its large, repeat-enriched genome makes genome assembly difficult and limits molecular breeding and biological studies. Herein, we present a chromosomal-level reference genome assembly of the double-haploid onion line DHW30006, constructed by combining PacBio, Illumina, and Hi-C sequencing approaches. The assembled genome totaled 12.77 Gb, with 65,730 gene models, and was anchored to eight pseudo-chromosomes covering 12.07 Gb (94.5%), with a scaffold N50 of 1.40 Gb. DHW30006 onion genome contained improved gene models covering approximately 580 Mb (4.54%) of the genic regions with an average gene length of 8,827 bp and 5.48 exons per gene. These gene models represented the most improved annotation among Allium genomes. This onion genome will serve as a valuable resource for breeding and biological research in Allium plants.
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issn 2052-4463
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spelling doaj-art-57deb22d3f6448299c5b1fa7976189052025-08-20T02:59:35ZengNature PortfolioScientific Data2052-44632025-02-011211910.1038/s41597-025-04635-3Chromosome-level genome assembly and improved annotation of onion genome (Allium cepa L.)Heejung Cho0Myunghee Jung1Seung Jae Lee2Ji Yeon Park3Yedomon Ange Bovys Zoclanclounon4Cheol-Woo Kim5JiWon Han6Jung Sun Kim7Do-Sun Kim8Younhee Shin9Yoon-Jung Hwang10Tae-Ho Lee11Si Myung Lee12Sang-Ho Kang13So Youn Won14Jin-Hyun Kim15Hye Yoon Jang16Hye-Eun Lee17Eun Su Lee18Sang-Choon Lee19Hyeonso Ji20Seong-Han Sohn21Byoung Ohg Ahn22Genomics Division, National Institute of Agricultural Sciences, RDAResearch and Development Center, Insilicogen, Inc.DNA Link Inc.Genomics Division, National Institute of Agricultural Sciences, RDAGenomics Division, National Institute of Agricultural Sciences, RDAAllium Vegetable Research Center, National Institute of Horticultural and Herbal Science, RDAAllium Vegetable Research Center, National Institute of Horticultural and Herbal Science, RDAGenomics Division, National Institute of Agricultural Sciences, RDAVegetable Division, National Institute of Horticultural and Herbal Science, RDAResearch and Development Center, Insilicogen, Inc.Department of Chemistry Life Science, Sahmyook UniversityGenomics Division, National Institute of Agricultural Sciences, RDAGenomics Division, National Institute of Agricultural Sciences, RDAGenomics Division, National Institute of Agricultural Sciences, RDAGenomics Division, National Institute of Agricultural Sciences, RDAGenomics Division, National Institute of Agricultural Sciences, RDADNA Link Inc.Vegetable Division, National Institute of Horticultural and Herbal Science, RDAVegetable Division, National Institute of Horticultural and Herbal Science, RDAPhyzen Genomics InstituteGene Engineering Division, National Institute of Agricultural Sciences, RDAGenomics Division, National Institute of Agricultural Sciences, RDAGenomics Division, National Institute of Agricultural Sciences, RDAAbstract Onion (Allium cepa L.) is an economically valuable crop, but its large, repeat-enriched genome makes genome assembly difficult and limits molecular breeding and biological studies. Herein, we present a chromosomal-level reference genome assembly of the double-haploid onion line DHW30006, constructed by combining PacBio, Illumina, and Hi-C sequencing approaches. The assembled genome totaled 12.77 Gb, with 65,730 gene models, and was anchored to eight pseudo-chromosomes covering 12.07 Gb (94.5%), with a scaffold N50 of 1.40 Gb. DHW30006 onion genome contained improved gene models covering approximately 580 Mb (4.54%) of the genic regions with an average gene length of 8,827 bp and 5.48 exons per gene. These gene models represented the most improved annotation among Allium genomes. This onion genome will serve as a valuable resource for breeding and biological research in Allium plants.https://doi.org/10.1038/s41597-025-04635-3
spellingShingle Heejung Cho
Myunghee Jung
Seung Jae Lee
Ji Yeon Park
Yedomon Ange Bovys Zoclanclounon
Cheol-Woo Kim
JiWon Han
Jung Sun Kim
Do-Sun Kim
Younhee Shin
Yoon-Jung Hwang
Tae-Ho Lee
Si Myung Lee
Sang-Ho Kang
So Youn Won
Jin-Hyun Kim
Hye Yoon Jang
Hye-Eun Lee
Eun Su Lee
Sang-Choon Lee
Hyeonso Ji
Seong-Han Sohn
Byoung Ohg Ahn
Chromosome-level genome assembly and improved annotation of onion genome (Allium cepa L.)
Scientific Data
title Chromosome-level genome assembly and improved annotation of onion genome (Allium cepa L.)
title_full Chromosome-level genome assembly and improved annotation of onion genome (Allium cepa L.)
title_fullStr Chromosome-level genome assembly and improved annotation of onion genome (Allium cepa L.)
title_full_unstemmed Chromosome-level genome assembly and improved annotation of onion genome (Allium cepa L.)
title_short Chromosome-level genome assembly and improved annotation of onion genome (Allium cepa L.)
title_sort chromosome level genome assembly and improved annotation of onion genome allium cepa l
url https://doi.org/10.1038/s41597-025-04635-3
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