Succinate dehydrogenase governs the sensitivity to SDHI fungicides and fruit pathogenicity of Colletotrichum gloeosporioides
Succinate dehydrogenase inhibitor (SDHI) fungicides are widely used to control plant anthracnose caused by Colletotrichum gloeosporioides. However, C. gloeosporioides resistant to SDHI fungicides have emerged in the orchard, also carry significant environmental risks. The thifluzamide, boscalid, and...
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Elsevier
2025-03-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154324006240 |
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| author | He Zhang Zi Ye Shiyou Yang Mengting Zhang Yang Xia Zhixin Liu Yu Sun Jian Li Xi Li Zhengjie Zhu Jinji Pu |
| author_facet | He Zhang Zi Ye Shiyou Yang Mengting Zhang Yang Xia Zhixin Liu Yu Sun Jian Li Xi Li Zhengjie Zhu Jinji Pu |
| author_sort | He Zhang |
| collection | DOAJ |
| description | Succinate dehydrogenase inhibitor (SDHI) fungicides are widely used to control plant anthracnose caused by Colletotrichum gloeosporioides. However, C. gloeosporioides resistant to SDHI fungicides have emerged in the orchard, also carry significant environmental risks. The thifluzamide, boscalid, and fluopyram are commonly used SDHIs fungicides in plant protection. In the study, the inhibitory activity of thifluzamide, boscalid, fluopyram against 55 C. gloeosporioides strains from China were determined, including mycelial growth and conidial production. The three SDHIs fungicides could inhibit the growth of mycelia and production of conidia of C. gloeosporioides. Here, we identified SDH subunits of C. gloeosporioides and expression patterns, and obtained CgSDHB, CgSDHC, CgSDHD mutants of C. gloeosporioides using CRISPR/Cas9 gene editing system. Biochemical evidence suggests that the mutants have reduced tricarboxylic acid cycle efficiency, thereby increasing the susceptibility of SDHIs and decreasing pathogenicity to multiple hosts. These results improve our understanding of SDHIs antifungal mechanism in C. gloeosporioides and provide new insights into the pathogenesis of SDH subunits regulation. |
| format | Article |
| id | doaj-art-57db0cefed3d464b94ec8e581d28ec2d |
| institution | DOAJ |
| issn | 2666-1543 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-57db0cefed3d464b94ec8e581d28ec2d2025-08-20T03:12:51ZengElsevierJournal of Agriculture and Food Research2666-15432025-03-011910158710.1016/j.jafr.2024.101587Succinate dehydrogenase governs the sensitivity to SDHI fungicides and fruit pathogenicity of Colletotrichum gloeosporioidesHe Zhang0Zi Ye1Shiyou Yang2Mengting Zhang3Yang Xia4Zhixin Liu5Yu Sun6Jian Li7Xi Li8Zhengjie Zhu9Jinji Pu10National Key Laboratory for Tropical Crop Breeding, Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture and Rural Affairs, Chinese Academy of Tropical Agricultural Sciences Environment and Plant Protection Institute, Haikou, 571101, China; Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China; Corresponding author. National Key Laboratory for Tropical Crop Breeding, Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture and Rural Affairs, Chinese Academy of Tropical Agricultural Sciences Environment and Plant Protection Institute, Haikou, 571101, China.National Key Laboratory for Tropical Crop Breeding, Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture and Rural Affairs, Chinese Academy of Tropical Agricultural Sciences Environment and Plant Protection Institute, Haikou, 571101, China; Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China; Honghe University, Mengzi, 661199, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, ChinaNational Key Laboratory for Tropical Crop Breeding, Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture and Rural Affairs, Chinese Academy of Tropical Agricultural Sciences Environment and Plant Protection Institute, Haikou, 571101, ChinaNational Key Laboratory for Tropical Crop Breeding, Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture and Rural Affairs, Chinese Academy of Tropical Agricultural Sciences Environment and Plant Protection Institute, Haikou, 571101, ChinaNational Key Laboratory for Tropical Crop Breeding, Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture and Rural Affairs, Chinese Academy of Tropical Agricultural Sciences Environment and Plant Protection Institute, Haikou, 571101, ChinaGuangxi Key Laboratory of Biology for Mango, College of Agriculture and Food Engineering, Baise University, Baise, 533000, ChinaGuangxi Key Laboratory of Biology for Mango, College of Agriculture and Food Engineering, Baise University, Baise, 533000, ChinaGuangxi Key Laboratory of Biology for Mango, College of Agriculture and Food Engineering, Baise University, Baise, 533000, China; Corresponding author.National Key Laboratory for Tropical Crop Breeding, Key Laboratory of Integrated Pest Management on Tropical Grops, Ministry of Agriculture and Rural Affairs, Chinese Academy of Tropical Agricultural Sciences Environment and Plant Protection Institute, Haikou, 571101, China; Corresponding author.Succinate dehydrogenase inhibitor (SDHI) fungicides are widely used to control plant anthracnose caused by Colletotrichum gloeosporioides. However, C. gloeosporioides resistant to SDHI fungicides have emerged in the orchard, also carry significant environmental risks. The thifluzamide, boscalid, and fluopyram are commonly used SDHIs fungicides in plant protection. In the study, the inhibitory activity of thifluzamide, boscalid, fluopyram against 55 C. gloeosporioides strains from China were determined, including mycelial growth and conidial production. The three SDHIs fungicides could inhibit the growth of mycelia and production of conidia of C. gloeosporioides. Here, we identified SDH subunits of C. gloeosporioides and expression patterns, and obtained CgSDHB, CgSDHC, CgSDHD mutants of C. gloeosporioides using CRISPR/Cas9 gene editing system. Biochemical evidence suggests that the mutants have reduced tricarboxylic acid cycle efficiency, thereby increasing the susceptibility of SDHIs and decreasing pathogenicity to multiple hosts. These results improve our understanding of SDHIs antifungal mechanism in C. gloeosporioides and provide new insights into the pathogenesis of SDH subunits regulation.http://www.sciencedirect.com/science/article/pii/S2666154324006240CRISPR/Cas9 gene editing systemInhibitory activityMycelial growthPathogenicityBiochemical evidenceSDH subunits |
| spellingShingle | He Zhang Zi Ye Shiyou Yang Mengting Zhang Yang Xia Zhixin Liu Yu Sun Jian Li Xi Li Zhengjie Zhu Jinji Pu Succinate dehydrogenase governs the sensitivity to SDHI fungicides and fruit pathogenicity of Colletotrichum gloeosporioides Journal of Agriculture and Food Research CRISPR/Cas9 gene editing system Inhibitory activity Mycelial growth Pathogenicity Biochemical evidence SDH subunits |
| title | Succinate dehydrogenase governs the sensitivity to SDHI fungicides and fruit pathogenicity of Colletotrichum gloeosporioides |
| title_full | Succinate dehydrogenase governs the sensitivity to SDHI fungicides and fruit pathogenicity of Colletotrichum gloeosporioides |
| title_fullStr | Succinate dehydrogenase governs the sensitivity to SDHI fungicides and fruit pathogenicity of Colletotrichum gloeosporioides |
| title_full_unstemmed | Succinate dehydrogenase governs the sensitivity to SDHI fungicides and fruit pathogenicity of Colletotrichum gloeosporioides |
| title_short | Succinate dehydrogenase governs the sensitivity to SDHI fungicides and fruit pathogenicity of Colletotrichum gloeosporioides |
| title_sort | succinate dehydrogenase governs the sensitivity to sdhi fungicides and fruit pathogenicity of colletotrichum gloeosporioides |
| topic | CRISPR/Cas9 gene editing system Inhibitory activity Mycelial growth Pathogenicity Biochemical evidence SDH subunits |
| url | http://www.sciencedirect.com/science/article/pii/S2666154324006240 |
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