Heat therapy preserves myofibre size and SERCA‐mediated Ca2+ uptake in the mouse soleus after tenotomy surgery
Abstract Heat therapy (HT) has been shown to induce physiological adaptations in muscle, including a reduction in the severity of muscle atrophy resulting from unloading. The muscle atrophy caused by unloading can be partially attributed to the dysregulation of Ca2+ in the muscle cell, which can act...
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2025-05-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.70385 |
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| author | Michael K. Barfoot Jessica L. Braun Phillip J. Wallace Bianca M. Marcella Ryan W. Baranowski Rebecca E. K. MacPherson Stephen S. Cheung Val A. Fajardo |
| author_facet | Michael K. Barfoot Jessica L. Braun Phillip J. Wallace Bianca M. Marcella Ryan W. Baranowski Rebecca E. K. MacPherson Stephen S. Cheung Val A. Fajardo |
| author_sort | Michael K. Barfoot |
| collection | DOAJ |
| description | Abstract Heat therapy (HT) has been shown to induce physiological adaptations in muscle, including a reduction in the severity of muscle atrophy resulting from unloading. The muscle atrophy caused by unloading can be partially attributed to the dysregulation of Ca2+ in the muscle cell, which can activate calpain‐mediated proteolysis. The sarco(endo)plasmic reticulum Ca2+‐ATPase (SERCA) is a primary regulator of Ca2+ in muscle, and SERCA dysfunction has been repeatedly demonstrated in various models of muscle unloading. Heat shock protein 70 (HSP70) is a heat‐inducible chaperone protein that binds to SERCA and protects against its dysfunction. While previous research has shown HT to upregulate HSP70 in rodent muscle, even in the unloaded state, the effects of HT on SERCA function in rodent skeletal muscle under these conditions remain unknown. Here, we characterized the effects of 4 weeks of HT on soleus muscle size, HSP70 expression, SERCA function, and maximal calpain activity in male C57BL/6J mice subjected to muscle unloading through tenotomy. Four weeks of HT preserved the cross‐sectional area of soleus myofibres following tenotomy, while also upregulating HSP70, maintaining SERCA‐mediated Ca2+ uptake, and reducing maximal calpain activity. Therefore, our research offers new insights into the advantages of HT for muscle health and physiology. |
| format | Article |
| id | doaj-art-57cfc043497d4046966d012c62dd763b |
| institution | Kabale University |
| issn | 2051-817X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj-art-57cfc043497d4046966d012c62dd763b2025-08-20T04:27:55ZengWileyPhysiological Reports2051-817X2025-05-011310n/an/a10.14814/phy2.70385Heat therapy preserves myofibre size and SERCA‐mediated Ca2+ uptake in the mouse soleus after tenotomy surgeryMichael K. Barfoot0Jessica L. Braun1Phillip J. Wallace2Bianca M. Marcella3Ryan W. Baranowski4Rebecca E. K. MacPherson5Stephen S. Cheung6Val A. Fajardo7Department of Kinesiology Brock University St. Catharines Ontario CanadaDepartment of Kinesiology Brock University St. Catharines Ontario CanadaDepartment of Kinesiology Brock University St. Catharines Ontario CanadaDepartment of Kinesiology Brock University St. Catharines Ontario CanadaDepartment of Kinesiology Brock University St. Catharines Ontario CanadaCentre for Bone and Muscle Health Brock University St. Catharines Ontario CanadaDepartment of Kinesiology Brock University St. Catharines Ontario CanadaDepartment of Kinesiology Brock University St. Catharines Ontario CanadaAbstract Heat therapy (HT) has been shown to induce physiological adaptations in muscle, including a reduction in the severity of muscle atrophy resulting from unloading. The muscle atrophy caused by unloading can be partially attributed to the dysregulation of Ca2+ in the muscle cell, which can activate calpain‐mediated proteolysis. The sarco(endo)plasmic reticulum Ca2+‐ATPase (SERCA) is a primary regulator of Ca2+ in muscle, and SERCA dysfunction has been repeatedly demonstrated in various models of muscle unloading. Heat shock protein 70 (HSP70) is a heat‐inducible chaperone protein that binds to SERCA and protects against its dysfunction. While previous research has shown HT to upregulate HSP70 in rodent muscle, even in the unloaded state, the effects of HT on SERCA function in rodent skeletal muscle under these conditions remain unknown. Here, we characterized the effects of 4 weeks of HT on soleus muscle size, HSP70 expression, SERCA function, and maximal calpain activity in male C57BL/6J mice subjected to muscle unloading through tenotomy. Four weeks of HT preserved the cross‐sectional area of soleus myofibres following tenotomy, while also upregulating HSP70, maintaining SERCA‐mediated Ca2+ uptake, and reducing maximal calpain activity. Therefore, our research offers new insights into the advantages of HT for muscle health and physiology.https://doi.org/10.14814/phy2.70385calpainHSP70muscle unloadingSERCA |
| spellingShingle | Michael K. Barfoot Jessica L. Braun Phillip J. Wallace Bianca M. Marcella Ryan W. Baranowski Rebecca E. K. MacPherson Stephen S. Cheung Val A. Fajardo Heat therapy preserves myofibre size and SERCA‐mediated Ca2+ uptake in the mouse soleus after tenotomy surgery Physiological Reports calpain HSP70 muscle unloading SERCA |
| title | Heat therapy preserves myofibre size and SERCA‐mediated Ca2+ uptake in the mouse soleus after tenotomy surgery |
| title_full | Heat therapy preserves myofibre size and SERCA‐mediated Ca2+ uptake in the mouse soleus after tenotomy surgery |
| title_fullStr | Heat therapy preserves myofibre size and SERCA‐mediated Ca2+ uptake in the mouse soleus after tenotomy surgery |
| title_full_unstemmed | Heat therapy preserves myofibre size and SERCA‐mediated Ca2+ uptake in the mouse soleus after tenotomy surgery |
| title_short | Heat therapy preserves myofibre size and SERCA‐mediated Ca2+ uptake in the mouse soleus after tenotomy surgery |
| title_sort | heat therapy preserves myofibre size and serca mediated ca2 uptake in the mouse soleus after tenotomy surgery |
| topic | calpain HSP70 muscle unloading SERCA |
| url | https://doi.org/10.14814/phy2.70385 |
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