EARLY HISTOPATHOLOGICAL COMPARISON OF SINGLE VERSUS DOUBLE-LAYER CLOSURE OF UTERINE INCISION DURING CESAREAN: A CONTROLLED STUDY IN RATS

Objective: Long-term complications of cesarean delivery are numerous and are increasing due to the high cesarean rate. The long-term complications are attributed to the different suturing methods. The single-layer locked (SLL) and double-layerunlocked (DLU) are the most compared. This study aims to...

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Main Authors: Merve Civelek, Selim Büyükkurt, Mehmet Bertan Yılmaz, Kıvılcım Eren Erdoğan, Hale Öksüz, Çağrı Avcı, Gülşah Seydaoğlu
Format: Article
Language:English
Published: Istanbul University Press 2023-08-01
Series:İstanbul Tıp Fakültesi Dergisi
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Online Access:https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/95C52ED142E249F08EF2242B973F15AC
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author Merve Civelek
Selim Büyükkurt
Mehmet Bertan Yılmaz
Kıvılcım Eren Erdoğan
Hale Öksüz
Çağrı Avcı
Gülşah Seydaoğlu
author_facet Merve Civelek
Selim Büyükkurt
Mehmet Bertan Yılmaz
Kıvılcım Eren Erdoğan
Hale Öksüz
Çağrı Avcı
Gülşah Seydaoğlu
author_sort Merve Civelek
collection DOAJ
description Objective: Long-term complications of cesarean delivery are numerous and are increasing due to the high cesarean rate. The long-term complications are attributed to the different suturing methods. The single-layer locked (SLL) and double-layerunlocked (DLU) are the most compared. This study aims to compare the early histopathological properties of these two suture techniques in an experimental animal study.Material and Method: Ten pregnant Wistar-Albino rats had cesarean at term. The right uterine horns were repaired using the SLL suture technique, and the left ones were repaired using the DLU technique. The control subjects were the six uterine horns of three pregnant rats delivered vaginally. Each animal underwent a hysterectomy on day 15 of pregnancy, and each uterus was an experimental unit. The mean thickness of the endometrium and myometrium of the intervention groups with histopathological features and controls were compared.Result: In SLL and DLU groups, the endometrium (276.6±123.1vs. 258.4±125.9 μm; p=0.748) and myometrium (531.7±189.2 vs. 505.9±182.1 μm; p=0.612) in scarred areas were similar. In nonscarred areas, the endometrium was measured as 239.9±68.9, 256.5±127.2, and 316.4±98.6 μm in SLL, DLU, and control group, respectively (p=0.347). The myometrium of non-scarred areas was also similar in all groups evaluated (387.2±119.9; 364.5±154.1 and 234.0±49.1 μm; p=0.265). The histopathological features of uteri repaired with two different techniques were also similar.Conclusion: The early histopathological properties of all uteri examined were similar after repairing with SLL or DLU techniques. These results indicate that the repair process does not differ in the early stages, regardless of the uterine closure technique used.
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series İstanbul Tıp Fakültesi Dergisi
spelling doaj-art-57ca888460eb40a09c19f0130affd8e92025-08-20T03:52:47ZengIstanbul University Pressİstanbul Tıp Fakültesi Dergisi1305-64412023-08-0186322022610.26650/IUITFD.1261550123456EARLY HISTOPATHOLOGICAL COMPARISON OF SINGLE VERSUS DOUBLE-LAYER CLOSURE OF UTERINE INCISION DURING CESAREAN: A CONTROLLED STUDY IN RATSMerve Civelek0https://orcid.org/0000-0002-2767-2224Selim Büyükkurt1https://orcid.org/0000-0003-0572-254XMehmet Bertan Yılmaz2https://orcid.org/0000-0001-5558-3299Kıvılcım Eren Erdoğan3https://orcid.org/0000-0002-4951-8703Hale Öksüz4https://orcid.org/0000-0003-2191-4066Çağrı Avcı5https://orcid.org/0000-0002-7762-2874Gülşah Seydaoğlu6https://orcid.org/0000-0002-0899-894XÇukurova Üniversitesi, Adana, TurkiyeÇukurova Üniversitesi, Adana, TurkiyeÇukurova Üniversitesi, Adana, TurkiyeÇukurova Üniversitesi, Adana, TurkiyeÇukurova Üniversitesi, Adana, TurkiyeÇukurova Üniversitesi, Adana, TurkiyeÇukurova Üniversitesi, Adana, TurkiyeObjective: Long-term complications of cesarean delivery are numerous and are increasing due to the high cesarean rate. The long-term complications are attributed to the different suturing methods. The single-layer locked (SLL) and double-layerunlocked (DLU) are the most compared. This study aims to compare the early histopathological properties of these two suture techniques in an experimental animal study.Material and Method: Ten pregnant Wistar-Albino rats had cesarean at term. The right uterine horns were repaired using the SLL suture technique, and the left ones were repaired using the DLU technique. The control subjects were the six uterine horns of three pregnant rats delivered vaginally. Each animal underwent a hysterectomy on day 15 of pregnancy, and each uterus was an experimental unit. The mean thickness of the endometrium and myometrium of the intervention groups with histopathological features and controls were compared.Result: In SLL and DLU groups, the endometrium (276.6±123.1vs. 258.4±125.9 μm; p=0.748) and myometrium (531.7±189.2 vs. 505.9±182.1 μm; p=0.612) in scarred areas were similar. In nonscarred areas, the endometrium was measured as 239.9±68.9, 256.5±127.2, and 316.4±98.6 μm in SLL, DLU, and control group, respectively (p=0.347). The myometrium of non-scarred areas was also similar in all groups evaluated (387.2±119.9; 364.5±154.1 and 234.0±49.1 μm; p=0.265). The histopathological features of uteri repaired with two different techniques were also similar.Conclusion: The early histopathological properties of all uteri examined were similar after repairing with SLL or DLU techniques. These results indicate that the repair process does not differ in the early stages, regardless of the uterine closure technique used.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/95C52ED142E249F08EF2242B973F15ACsurgical techniqueuterine closurecesarean sectionpregnancyhistopathology
spellingShingle Merve Civelek
Selim Büyükkurt
Mehmet Bertan Yılmaz
Kıvılcım Eren Erdoğan
Hale Öksüz
Çağrı Avcı
Gülşah Seydaoğlu
EARLY HISTOPATHOLOGICAL COMPARISON OF SINGLE VERSUS DOUBLE-LAYER CLOSURE OF UTERINE INCISION DURING CESAREAN: A CONTROLLED STUDY IN RATS
İstanbul Tıp Fakültesi Dergisi
surgical technique
uterine closure
cesarean section
pregnancy
histopathology
title EARLY HISTOPATHOLOGICAL COMPARISON OF SINGLE VERSUS DOUBLE-LAYER CLOSURE OF UTERINE INCISION DURING CESAREAN: A CONTROLLED STUDY IN RATS
title_full EARLY HISTOPATHOLOGICAL COMPARISON OF SINGLE VERSUS DOUBLE-LAYER CLOSURE OF UTERINE INCISION DURING CESAREAN: A CONTROLLED STUDY IN RATS
title_fullStr EARLY HISTOPATHOLOGICAL COMPARISON OF SINGLE VERSUS DOUBLE-LAYER CLOSURE OF UTERINE INCISION DURING CESAREAN: A CONTROLLED STUDY IN RATS
title_full_unstemmed EARLY HISTOPATHOLOGICAL COMPARISON OF SINGLE VERSUS DOUBLE-LAYER CLOSURE OF UTERINE INCISION DURING CESAREAN: A CONTROLLED STUDY IN RATS
title_short EARLY HISTOPATHOLOGICAL COMPARISON OF SINGLE VERSUS DOUBLE-LAYER CLOSURE OF UTERINE INCISION DURING CESAREAN: A CONTROLLED STUDY IN RATS
title_sort early histopathological comparison of single versus double layer closure of uterine incision during cesarean a controlled study in rats
topic surgical technique
uterine closure
cesarean section
pregnancy
histopathology
url https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/95C52ED142E249F08EF2242B973F15AC
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AT selimbuyukkurt earlyhistopathologicalcomparisonofsingleversusdoublelayerclosureofuterineincisionduringcesareanacontrolledstudyinrats
AT mehmetbertanyılmaz earlyhistopathologicalcomparisonofsingleversusdoublelayerclosureofuterineincisionduringcesareanacontrolledstudyinrats
AT kıvılcımerenerdogan earlyhistopathologicalcomparisonofsingleversusdoublelayerclosureofuterineincisionduringcesareanacontrolledstudyinrats
AT haleoksuz earlyhistopathologicalcomparisonofsingleversusdoublelayerclosureofuterineincisionduringcesareanacontrolledstudyinrats
AT cagrıavcı earlyhistopathologicalcomparisonofsingleversusdoublelayerclosureofuterineincisionduringcesareanacontrolledstudyinrats
AT gulsahseydaoglu earlyhistopathologicalcomparisonofsingleversusdoublelayerclosureofuterineincisionduringcesareanacontrolledstudyinrats