Spectrophotometric Determination of Sulphadiazine Using 2,4 –dinitrophenylhydrazine as Coupling Reagent
A rapid, sensitive and selective spectrophotometric method was developed for determination of sulphadiazine in aqueous solution. The method is based on the oxidative coupling reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH) in a basic medium in the presence of potassium periodate to produce an...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tikrit University
2018-08-01
|
| Series: | Tikrit Journal of Pure Science |
| Subjects: | |
| Online Access: | https://tjpsj.org/index.php/tjps/article/view/546 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850114118367838208 |
|---|---|
| author | Najlaa Abd AL Salam Ahmed Ali Ibraheem Khaleel |
| author_facet | Najlaa Abd AL Salam Ahmed Ali Ibraheem Khaleel |
| author_sort | Najlaa Abd AL Salam Ahmed |
| collection | DOAJ |
| description |
A rapid, sensitive and selective spectrophotometric method was developed for determination of sulphadiazine in aqueous solution. The method is based on the oxidative coupling reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH) in a basic medium in the presence of potassium periodate to produce an intense orange colour product, soluble in water, stable and absorbs at 486 nm. The linearity of Beer's law is in the range 3-15μg/ml of sulphadiazine. The molar absorptivity, Sandellʼs sensitivity index, and detection limit were 2.23x104liter.mol-1. cm-1, 0.0222 μg.cm-2 and 0.0307μg/ml respectively. The RSD value was 0.506 – 0.116 % depending on the concentration value. This method is applied successfully to the determination of sulphadiazine veterinary injection liquid solution (VAPCOTRIM) with average recovery of not less than 99.5%.
|
| format | Article |
| id | doaj-art-57c97ea008754cbb8c14a7726bd6f482 |
| institution | OA Journals |
| issn | 1813-1662 2415-1726 |
| language | English |
| publishDate | 2018-08-01 |
| publisher | Tikrit University |
| record_format | Article |
| series | Tikrit Journal of Pure Science |
| spelling | doaj-art-57c97ea008754cbb8c14a7726bd6f4822025-08-20T02:36:59ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262018-08-0123810.25130/tjps.v23i8.546Spectrophotometric Determination of Sulphadiazine Using 2,4 –dinitrophenylhydrazine as Coupling ReagentNajlaa Abd AL Salam AhmedAli Ibraheem Khaleel A rapid, sensitive and selective spectrophotometric method was developed for determination of sulphadiazine in aqueous solution. The method is based on the oxidative coupling reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH) in a basic medium in the presence of potassium periodate to produce an intense orange colour product, soluble in water, stable and absorbs at 486 nm. The linearity of Beer's law is in the range 3-15μg/ml of sulphadiazine. The molar absorptivity, Sandellʼs sensitivity index, and detection limit were 2.23x104liter.mol-1. cm-1, 0.0222 μg.cm-2 and 0.0307μg/ml respectively. The RSD value was 0.506 – 0.116 % depending on the concentration value. This method is applied successfully to the determination of sulphadiazine veterinary injection liquid solution (VAPCOTRIM) with average recovery of not less than 99.5%. https://tjpsj.org/index.php/tjps/article/view/546Spectrophotometricsulphadiazine2,4- dinitrophenylhydrazine (2,4-DNPH) |
| spellingShingle | Najlaa Abd AL Salam Ahmed Ali Ibraheem Khaleel Spectrophotometric Determination of Sulphadiazine Using 2,4 –dinitrophenylhydrazine as Coupling Reagent Tikrit Journal of Pure Science Spectrophotometric sulphadiazine 2,4- dinitrophenylhydrazine (2,4-DNPH) |
| title | Spectrophotometric Determination of Sulphadiazine Using 2,4 –dinitrophenylhydrazine as Coupling Reagent |
| title_full | Spectrophotometric Determination of Sulphadiazine Using 2,4 –dinitrophenylhydrazine as Coupling Reagent |
| title_fullStr | Spectrophotometric Determination of Sulphadiazine Using 2,4 –dinitrophenylhydrazine as Coupling Reagent |
| title_full_unstemmed | Spectrophotometric Determination of Sulphadiazine Using 2,4 –dinitrophenylhydrazine as Coupling Reagent |
| title_short | Spectrophotometric Determination of Sulphadiazine Using 2,4 –dinitrophenylhydrazine as Coupling Reagent |
| title_sort | spectrophotometric determination of sulphadiazine using 2 4 dinitrophenylhydrazine as coupling reagent |
| topic | Spectrophotometric sulphadiazine 2,4- dinitrophenylhydrazine (2,4-DNPH) |
| url | https://tjpsj.org/index.php/tjps/article/view/546 |
| work_keys_str_mv | AT najlaaabdalsalamahmed spectrophotometricdeterminationofsulphadiazineusing24dinitrophenylhydrazineascouplingreagent AT aliibraheemkhaleel spectrophotometricdeterminationofsulphadiazineusing24dinitrophenylhydrazineascouplingreagent |