Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis
A green, cost-effective, and simple capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of chloramphenicol, methylparaben, and propylparaben in eye-drops. With sodium tetraborate as background electrolyte (BGE), the apparent mobilities of chloramphe...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2021/5575701 |
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| author | Thi Thanh Vuong Tong Thi Thoa Cao Nguyen Ha Tran Thi Kim Van Le Dinh Chi Le |
| author_facet | Thi Thanh Vuong Tong Thi Thoa Cao Nguyen Ha Tran Thi Kim Van Le Dinh Chi Le |
| author_sort | Thi Thanh Vuong Tong |
| collection | DOAJ |
| description | A green, cost-effective, and simple capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of chloramphenicol, methylparaben, and propylparaben in eye-drops. With sodium tetraborate as background electrolyte (BGE), the apparent mobilities of chloramphenicol, methylparaben, and propylparaben increased and analysis time reduced when pH of BGE increased from 8.5 to 10.0 and concentration of BGE decreased from 40 mM to 15 mM, but complete separation of chloramphenicol from other matrix components was achieved only with sodium tetraborate concentration at 30 mM or higher and at pH = 9.3 or lower. The most suitable electrophoretic conditions for the intended application were a 30 mM sodium tetraborate solution, pH 9.3 as BGE, working voltage set at 25 kV, and UV detection at 280 nm at the cathodic extremity of the capillary. The final method was validated and proved to be reliable for assay of chloramphenicol, methylparaben, and propylparaben in eye-drops. |
| format | Article |
| id | doaj-art-57c7dcf53a784d8ebb0be9622a39259d |
| institution | OA Journals |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
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| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-57c7dcf53a784d8ebb0be9622a39259d2025-08-20T02:05:47ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732021-01-01202110.1155/2021/55757015575701Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone ElectrophoresisThi Thanh Vuong Tong0Thi Thoa Cao1Nguyen Ha Tran2Thi Kim Van Le3Dinh Chi Le4Department of Analytical Chemistry and Toxicology, Hanoi University of Pharmacy, Hanoi, VietnamDepartment of Analytical Chemistry and Toxicology, Hanoi University of Pharmacy, Hanoi, VietnamDepartment of Analytical Chemistry and Toxicology, Hanoi University of Pharmacy, Hanoi, VietnamNational Institute of Medicinal Materials, Hanoi, VietnamNational Institute of Pharmaceutical Technology, Hanoi University of Pharmacy, Hanoi, VietnamA green, cost-effective, and simple capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of chloramphenicol, methylparaben, and propylparaben in eye-drops. With sodium tetraborate as background electrolyte (BGE), the apparent mobilities of chloramphenicol, methylparaben, and propylparaben increased and analysis time reduced when pH of BGE increased from 8.5 to 10.0 and concentration of BGE decreased from 40 mM to 15 mM, but complete separation of chloramphenicol from other matrix components was achieved only with sodium tetraborate concentration at 30 mM or higher and at pH = 9.3 or lower. The most suitable electrophoretic conditions for the intended application were a 30 mM sodium tetraborate solution, pH 9.3 as BGE, working voltage set at 25 kV, and UV detection at 280 nm at the cathodic extremity of the capillary. The final method was validated and proved to be reliable for assay of chloramphenicol, methylparaben, and propylparaben in eye-drops.http://dx.doi.org/10.1155/2021/5575701 |
| spellingShingle | Thi Thanh Vuong Tong Thi Thoa Cao Nguyen Ha Tran Thi Kim Van Le Dinh Chi Le Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis Journal of Analytical Methods in Chemistry |
| title | Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis |
| title_full | Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis |
| title_fullStr | Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis |
| title_full_unstemmed | Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis |
| title_short | Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis |
| title_sort | green cost effective simultaneous assay of chloramphenicol methylparaben and propylparaben in eye drops by capillary zone electrophoresis |
| url | http://dx.doi.org/10.1155/2021/5575701 |
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