Evolving Synergy Between Synthetic and Biotic Elements in Conjugated Polyelectrolyte/Bacteria Composite Improves Charge Transport and Mechanical Properties
Abstract gLiving materials can achieve unprecedented function by combining synthetic materials with the wide range of cellular functions. Of interest are situations where the critical properties of individual abiotic and biotic elements improve via their combination. For example, integrating electro...
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| Format: | Article |
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Wiley
2024-11-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202405242 |
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| author | Samantha R. McCuskey Glenn Quek Ricardo Javier Vázquez Binu Kundukad Muhammad Hafiz Bin Ismail Solange E. Astorga Yan Jiang Guillermo C. Bazan |
| author_facet | Samantha R. McCuskey Glenn Quek Ricardo Javier Vázquez Binu Kundukad Muhammad Hafiz Bin Ismail Solange E. Astorga Yan Jiang Guillermo C. Bazan |
| author_sort | Samantha R. McCuskey |
| collection | DOAJ |
| description | Abstract gLiving materials can achieve unprecedented function by combining synthetic materials with the wide range of cellular functions. Of interest are situations where the critical properties of individual abiotic and biotic elements improve via their combination. For example, integrating electroactive bacteria into conjugated polyelectrolyte (CPE) hydrogels increases biocurrent production. One observes more efficient electrical charge transport within the CPE matrix in the presence of Shewanella oneidensis MR‐1 and more current per cell is extracted, compared to traditional biofilms. Here, the origin of these synergistic effects are examined. Transcriptomics reveals that genes in S. oneidensis MR‐1 related to bacteriophages and energy metabolism are upregulated in the composite material. Fluorescent staining and rheological measurements before and after enzymatic treatment identified the importance of extracellular biomaterials in increasing matrix cohesion. The synergy between CPE and S. oneidensis MR‐1 thus arises from initially unanticipated changes in matrix composition and bacteria adaption within the synthetic environment. |
| format | Article |
| id | doaj-art-57c5a41feafe4db985f19ff1d1bdba71 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-57c5a41feafe4db985f19ff1d1bdba712025-08-20T03:52:43ZengWileyAdvanced Science2198-38442024-11-011142n/an/a10.1002/advs.202405242Evolving Synergy Between Synthetic and Biotic Elements in Conjugated Polyelectrolyte/Bacteria Composite Improves Charge Transport and Mechanical PropertiesSamantha R. McCuskey0Glenn Quek1Ricardo Javier Vázquez2Binu Kundukad3Muhammad Hafiz Bin Ismail4Solange E. Astorga5Yan Jiang6Guillermo C. Bazan7Department of Chemistry and Chemical & Biomolecular Engineering National University of Singapore Singapore 119077 SingaporeDepartment of Chemistry and Chemical & Biomolecular Engineering National University of Singapore Singapore 119077 SingaporeInstitute for Functional Intelligent Materials (I‐FIM) National University of Singapore Singapore 117544 SingaporeSingapore Centre on Environmental Life Sciences Engineering (SCELSE) Nanyang Technological University Singapore 637551 SingaporeSingapore Centre on Environmental Life Sciences Engineering (SCELSE) Nanyang Technological University Singapore 637551 SingaporeSingapore Centre on Environmental Life Sciences Engineering (SCELSE) Nanyang Technological University Singapore 637551 SingaporeDepartment of Chemistry and Chemical & Biomolecular Engineering National University of Singapore Singapore 119077 SingaporeDepartment of Chemistry and Chemical & Biomolecular Engineering National University of Singapore Singapore 119077 SingaporeAbstract gLiving materials can achieve unprecedented function by combining synthetic materials with the wide range of cellular functions. Of interest are situations where the critical properties of individual abiotic and biotic elements improve via their combination. For example, integrating electroactive bacteria into conjugated polyelectrolyte (CPE) hydrogels increases biocurrent production. One observes more efficient electrical charge transport within the CPE matrix in the presence of Shewanella oneidensis MR‐1 and more current per cell is extracted, compared to traditional biofilms. Here, the origin of these synergistic effects are examined. Transcriptomics reveals that genes in S. oneidensis MR‐1 related to bacteriophages and energy metabolism are upregulated in the composite material. Fluorescent staining and rheological measurements before and after enzymatic treatment identified the importance of extracellular biomaterials in increasing matrix cohesion. The synergy between CPE and S. oneidensis MR‐1 thus arises from initially unanticipated changes in matrix composition and bacteria adaption within the synthetic environment.https://doi.org/10.1002/advs.202405242abiotic/biotic interfacebioelectrochemical systemsconductive polymer hydrogelselectroactive bacteriaengineered living materialsextracellular polymeric substances |
| spellingShingle | Samantha R. McCuskey Glenn Quek Ricardo Javier Vázquez Binu Kundukad Muhammad Hafiz Bin Ismail Solange E. Astorga Yan Jiang Guillermo C. Bazan Evolving Synergy Between Synthetic and Biotic Elements in Conjugated Polyelectrolyte/Bacteria Composite Improves Charge Transport and Mechanical Properties Advanced Science abiotic/biotic interface bioelectrochemical systems conductive polymer hydrogels electroactive bacteria engineered living materials extracellular polymeric substances |
| title | Evolving Synergy Between Synthetic and Biotic Elements in Conjugated Polyelectrolyte/Bacteria Composite Improves Charge Transport and Mechanical Properties |
| title_full | Evolving Synergy Between Synthetic and Biotic Elements in Conjugated Polyelectrolyte/Bacteria Composite Improves Charge Transport and Mechanical Properties |
| title_fullStr | Evolving Synergy Between Synthetic and Biotic Elements in Conjugated Polyelectrolyte/Bacteria Composite Improves Charge Transport and Mechanical Properties |
| title_full_unstemmed | Evolving Synergy Between Synthetic and Biotic Elements in Conjugated Polyelectrolyte/Bacteria Composite Improves Charge Transport and Mechanical Properties |
| title_short | Evolving Synergy Between Synthetic and Biotic Elements in Conjugated Polyelectrolyte/Bacteria Composite Improves Charge Transport and Mechanical Properties |
| title_sort | evolving synergy between synthetic and biotic elements in conjugated polyelectrolyte bacteria composite improves charge transport and mechanical properties |
| topic | abiotic/biotic interface bioelectrochemical systems conductive polymer hydrogels electroactive bacteria engineered living materials extracellular polymeric substances |
| url | https://doi.org/10.1002/advs.202405242 |
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