Development and Breeding of Herbicide‐Resistant Sorghum for Effective Cereal‐Legume Intercropping
Abstract Weeds bring a serious challenge to crop production, and herbicides is the most effective and economic way to manage it in field. Sorghum is a critical crop for staple food, fodder, and biofuel. However, the lack of herbicide‐resistant sorghum germplasm severely impedes its production. Here,...
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Wiley
2025-07-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202503083 |
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| author | Sanyuan Tang Jiayang Shi Xuefeng Li Mingliang Yang Chao Li Dan Zhang Sen Yang Cuo Mei Zuyong Luo Li Zhang Wanke Zhang Chunrui Zhang Chenbo Zhu Xiaowei Ma Ran Xia Yuhang Chen Jinsong Zhang Qingshan Chen Shouyi Chen Qi Xie Feifei Yu |
| author_facet | Sanyuan Tang Jiayang Shi Xuefeng Li Mingliang Yang Chao Li Dan Zhang Sen Yang Cuo Mei Zuyong Luo Li Zhang Wanke Zhang Chunrui Zhang Chenbo Zhu Xiaowei Ma Ran Xia Yuhang Chen Jinsong Zhang Qingshan Chen Shouyi Chen Qi Xie Feifei Yu |
| author_sort | Sanyuan Tang |
| collection | DOAJ |
| description | Abstract Weeds bring a serious challenge to crop production, and herbicides is the most effective and economic way to manage it in field. Sorghum is a critical crop for staple food, fodder, and biofuel. However, the lack of herbicide‐resistant sorghum germplasm severely impedes its production. Here, we conducted a large‐scale screening and identified 13 sorghum mutant lines resistant to imidazolinone (IMI) herbicides. Two unique mutation sites in SbALS (acetolactate synthase), thus namely Sbals‐1 (A93T) and Sbals‐2 (S624N) are discovered, both enhance sorghum tolerance to imazamox. Notably, under high concentrations of imazamox, sbals‐1 presented a superior growth phenotype and elevated SbALS activity than sbals‐2, a difference that can be attributed to the predicted protein structures. Breeding with Sbals, both grain‐ and grass‐type sorghum, shows great weed control and field performance. The herbicide imazamox resistance is further evaluated in a soybean population for sorghum‐soybean strip intercropping, identifying 123 highly resistant soybean varieties. Field intercropping tests indicated health growth of both soybean and sorghum lines post‐imazamox treatment, which enhance field clearance of weed. This study, therefore, provides valuable insights not only for herbicide‐resistant sorghum breeding but also for the successful implementation of efficient and sustainable cereal‐legume intercropping systems. |
| format | Article |
| id | doaj-art-57c4aae1253c4da78388216da1c2b3c9 |
| institution | DOAJ |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-57c4aae1253c4da78388216da1c2b3c92025-08-20T03:12:04ZengWileyAdvanced Science2198-38442025-07-011227n/an/a10.1002/advs.202503083Development and Breeding of Herbicide‐Resistant Sorghum for Effective Cereal‐Legume IntercroppingSanyuan Tang0Jiayang Shi1Xuefeng Li2Mingliang Yang3Chao Li4Dan Zhang5Sen Yang6Cuo Mei7Zuyong Luo8Li Zhang9Wanke Zhang10Chunrui Zhang11Chenbo Zhu12Xiaowei Ma13Ran Xia14Yuhang Chen15Jinsong Zhang16Qingshan Chen17Shouyi Chen18Qi Xie19Feifei Yu20Institute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences Beijing 100101 ChinaInstitute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences Beijing 100101 ChinaCropedit Biotech Co., Ltd. Beijing 102206 ChinaCollege of Agriculture Northeast Agricultural University Harbin 150000 ChinaInstitute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences Beijing 100101 ChinaInstitute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences Beijing 100101 ChinaInstitute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences Beijing 100101 ChinaInstitute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences Beijing 100101 ChinaCropedit Biotech Co., Ltd. Beijing 102206 ChinaCropedit Biotech Co., Ltd. Beijing 102206 ChinaInstitute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences Beijing 100101 ChinaInstitute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences Beijing 100101 ChinaCollege of Agriculture Northeast Agricultural University Harbin 150000 ChinaNational Center of Technology Innovation for Maize State Key Laboratory of Crop Germplasm Innovation and Molecular Breeding Syngenta Group China Beijing 102206 ChinaInstitute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences Beijing 100101 ChinaInstitute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences Beijing 100101 ChinaInstitute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences Beijing 100101 ChinaCollege of Agriculture Northeast Agricultural University Harbin 150000 ChinaQilu Zhongke Academy of Modern Microbiology Technology Jinan 250018 ChinaInstitute of Genetics and Developmental Biology The Innovative Academy of Seed Design Chinese Academy of Sciences Beijing 100101 ChinaCollege of Grassland Science and Technology China Agricultural University Beijing 100083 ChinaAbstract Weeds bring a serious challenge to crop production, and herbicides is the most effective and economic way to manage it in field. Sorghum is a critical crop for staple food, fodder, and biofuel. However, the lack of herbicide‐resistant sorghum germplasm severely impedes its production. Here, we conducted a large‐scale screening and identified 13 sorghum mutant lines resistant to imidazolinone (IMI) herbicides. Two unique mutation sites in SbALS (acetolactate synthase), thus namely Sbals‐1 (A93T) and Sbals‐2 (S624N) are discovered, both enhance sorghum tolerance to imazamox. Notably, under high concentrations of imazamox, sbals‐1 presented a superior growth phenotype and elevated SbALS activity than sbals‐2, a difference that can be attributed to the predicted protein structures. Breeding with Sbals, both grain‐ and grass‐type sorghum, shows great weed control and field performance. The herbicide imazamox resistance is further evaluated in a soybean population for sorghum‐soybean strip intercropping, identifying 123 highly resistant soybean varieties. Field intercropping tests indicated health growth of both soybean and sorghum lines post‐imazamox treatment, which enhance field clearance of weed. This study, therefore, provides valuable insights not only for herbicide‐resistant sorghum breeding but also for the successful implementation of efficient and sustainable cereal‐legume intercropping systems.https://doi.org/10.1002/advs.202503083ALS/AHAScereal‐legume strip intercroppingherbicide‐resistant sorghumsoybean |
| spellingShingle | Sanyuan Tang Jiayang Shi Xuefeng Li Mingliang Yang Chao Li Dan Zhang Sen Yang Cuo Mei Zuyong Luo Li Zhang Wanke Zhang Chunrui Zhang Chenbo Zhu Xiaowei Ma Ran Xia Yuhang Chen Jinsong Zhang Qingshan Chen Shouyi Chen Qi Xie Feifei Yu Development and Breeding of Herbicide‐Resistant Sorghum for Effective Cereal‐Legume Intercropping Advanced Science ALS/AHAS cereal‐legume strip intercropping herbicide‐resistant sorghum soybean |
| title | Development and Breeding of Herbicide‐Resistant Sorghum for Effective Cereal‐Legume Intercropping |
| title_full | Development and Breeding of Herbicide‐Resistant Sorghum for Effective Cereal‐Legume Intercropping |
| title_fullStr | Development and Breeding of Herbicide‐Resistant Sorghum for Effective Cereal‐Legume Intercropping |
| title_full_unstemmed | Development and Breeding of Herbicide‐Resistant Sorghum for Effective Cereal‐Legume Intercropping |
| title_short | Development and Breeding of Herbicide‐Resistant Sorghum for Effective Cereal‐Legume Intercropping |
| title_sort | development and breeding of herbicide resistant sorghum for effective cereal legume intercropping |
| topic | ALS/AHAS cereal‐legume strip intercropping herbicide‐resistant sorghum soybean |
| url | https://doi.org/10.1002/advs.202503083 |
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