CCoAOMT positively regulates the biosynthesis of secondary metabolites in Zanthoxylum armatum
IntroductionThe rust disease caused by the fungus Coleosporium zanthoxyli in Zanthoxylum armatum leads to significant leaf damage. However, the genetic regulatory mechanisms underlying disease resistance in this plant remain unclear.MethodsThrough transcriptome analysis and bioinformatics screening,...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Agronomy |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fagro.2025.1604811/full |
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| author | Xiu Xu Yingxia Hu Yingming Lai Tianhui Zhu Tianhui Zhu Tianhui Zhu Shujiang Li Shujiang Li Shujiang Li Yinggao Liu Shuying Li Chunlin Yang Shan Han Shan Han Shan Han |
| author_facet | Xiu Xu Yingxia Hu Yingming Lai Tianhui Zhu Tianhui Zhu Tianhui Zhu Shujiang Li Shujiang Li Shujiang Li Yinggao Liu Shuying Li Chunlin Yang Shan Han Shan Han Shan Han |
| author_sort | Xiu Xu |
| collection | DOAJ |
| description | IntroductionThe rust disease caused by the fungus Coleosporium zanthoxyli in Zanthoxylum armatum leads to significant leaf damage. However, the genetic regulatory mechanisms underlying disease resistance in this plant remain unclear.MethodsThrough transcriptome analysis and bioinformatics screening, the candidate gene CCoAOMT was identified as potentially involved in rust resistance. Genetic engineering techniques were employed to construct recombinant plasmids for both overexpression and RNAi mediated of CCoAOMT. Transient transformation and stable transformation methods successfully generated CCoAOMT-RNAi and CCoAOMT-overexpression transgenic plants and calli, respectively.ResultsqRT-PCR analysis revealed that the expression levels of CCoAOMT and six related genes were downregulated in RNAi-silenced plants but upregulated in overexpression lines. Following inoculation with fungal spore suspensions, CCoAOMT-RNAi plants exhibited significantly higher disease incidence and severity indices compared to wild-type controls, whereas overexpression plants showed the opposite trend with reduced susceptibility. Furthermore, total lignin and flavonoid contents were markedly decreased in RNAi lines and increased in overexpression lines relative to wild-type plants. Subcellular localization assays using GFP fusion constructs confirmed dual localization of CCoAOMT protein in both the cytoplasm and nucleus.ConclusionThese findings collectively demonstrate that CCoAOMT enhances rust resistance in Z. armatum by regulating lignin and flavonoid biosynthesis pathways. |
| format | Article |
| id | doaj-art-57c2b4cfc0464b87ac10b79babe610cd |
| institution | DOAJ |
| issn | 2673-3218 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Agronomy |
| spelling | doaj-art-57c2b4cfc0464b87ac10b79babe610cd2025-08-20T02:44:59ZengFrontiers Media S.A.Frontiers in Agronomy2673-32182025-07-01710.3389/fagro.2025.16048111604811CCoAOMT positively regulates the biosynthesis of secondary metabolites in Zanthoxylum armatumXiu Xu0Yingxia Hu1Yingming Lai2Tianhui Zhu3Tianhui Zhu4Tianhui Zhu5Shujiang Li6Shujiang Li7Shujiang Li8Yinggao Liu9Shuying Li10Chunlin Yang11Shan Han12Shan Han13Shan Han14College of Forestry, Sichuan Agricultural University, Chengdu, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Forest Protection of Sichuan Education Department, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of National Forestry & Grassland Administration on Forest Resources Conservation and Ecological Safety in the Upper Reaches of the Yangtze River, Sichuan Agricultural University, Chengdu, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Forest Protection of Sichuan Education Department, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of National Forestry & Grassland Administration on Forest Resources Conservation and Ecological Safety in the Upper Reaches of the Yangtze River, Sichuan Agricultural University, Chengdu, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Forest Protection of Sichuan Education Department, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of National Forestry & Grassland Administration on Forest Resources Conservation and Ecological Safety in the Upper Reaches of the Yangtze River, Sichuan Agricultural University, Chengdu, ChinaIntroductionThe rust disease caused by the fungus Coleosporium zanthoxyli in Zanthoxylum armatum leads to significant leaf damage. However, the genetic regulatory mechanisms underlying disease resistance in this plant remain unclear.MethodsThrough transcriptome analysis and bioinformatics screening, the candidate gene CCoAOMT was identified as potentially involved in rust resistance. Genetic engineering techniques were employed to construct recombinant plasmids for both overexpression and RNAi mediated of CCoAOMT. Transient transformation and stable transformation methods successfully generated CCoAOMT-RNAi and CCoAOMT-overexpression transgenic plants and calli, respectively.ResultsqRT-PCR analysis revealed that the expression levels of CCoAOMT and six related genes were downregulated in RNAi-silenced plants but upregulated in overexpression lines. Following inoculation with fungal spore suspensions, CCoAOMT-RNAi plants exhibited significantly higher disease incidence and severity indices compared to wild-type controls, whereas overexpression plants showed the opposite trend with reduced susceptibility. Furthermore, total lignin and flavonoid contents were markedly decreased in RNAi lines and increased in overexpression lines relative to wild-type plants. Subcellular localization assays using GFP fusion constructs confirmed dual localization of CCoAOMT protein in both the cytoplasm and nucleus.ConclusionThese findings collectively demonstrate that CCoAOMT enhances rust resistance in Z. armatum by regulating lignin and flavonoid biosynthesis pathways.https://www.frontiersin.org/articles/10.3389/fagro.2025.1604811/fullZ. armatumresistance genesCCoAOMTRNAioverexpression |
| spellingShingle | Xiu Xu Yingxia Hu Yingming Lai Tianhui Zhu Tianhui Zhu Tianhui Zhu Shujiang Li Shujiang Li Shujiang Li Yinggao Liu Shuying Li Chunlin Yang Shan Han Shan Han Shan Han CCoAOMT positively regulates the biosynthesis of secondary metabolites in Zanthoxylum armatum Frontiers in Agronomy Z. armatum resistance genes CCoAOMT RNAi overexpression |
| title | CCoAOMT positively regulates the biosynthesis of secondary metabolites in Zanthoxylum armatum |
| title_full | CCoAOMT positively regulates the biosynthesis of secondary metabolites in Zanthoxylum armatum |
| title_fullStr | CCoAOMT positively regulates the biosynthesis of secondary metabolites in Zanthoxylum armatum |
| title_full_unstemmed | CCoAOMT positively regulates the biosynthesis of secondary metabolites in Zanthoxylum armatum |
| title_short | CCoAOMT positively regulates the biosynthesis of secondary metabolites in Zanthoxylum armatum |
| title_sort | ccoaomt positively regulates the biosynthesis of secondary metabolites in zanthoxylum armatum |
| topic | Z. armatum resistance genes CCoAOMT RNAi overexpression |
| url | https://www.frontiersin.org/articles/10.3389/fagro.2025.1604811/full |
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