MB09, a denosumab biosimilar candidate: Biosimilarity demonstration in a phase I study in healthy subjects

Abstract This was a Phase I, randomized, double‐blinded, three‐arm, single‐dose, parallel study aimed to demonstrate pharmacokinetic (PK) similarity between MB09 (a denosumab biosimilar candidate) and reference denosumab (XGEVA® from European Union [EU‐reference] and United States [US‐reference]) in...

Full description

Saved in:
Bibliographic Details
Main Authors: Monika Tomaszewska‐Kiecana, Elisabete Carapuça, Amalia Florez‐Igual, Javier Queiruga‐Parada
Format: Article
Language:English
Published: Wiley 2024-09-01
Series:Clinical and Translational Science
Online Access:https://doi.org/10.1111/cts.70013
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850262341820612608
author Monika Tomaszewska‐Kiecana
Elisabete Carapuça
Amalia Florez‐Igual
Javier Queiruga‐Parada
author_facet Monika Tomaszewska‐Kiecana
Elisabete Carapuça
Amalia Florez‐Igual
Javier Queiruga‐Parada
author_sort Monika Tomaszewska‐Kiecana
collection DOAJ
description Abstract This was a Phase I, randomized, double‐blinded, three‐arm, single‐dose, parallel study aimed to demonstrate pharmacokinetic (PK) similarity between MB09 (a denosumab biosimilar candidate) and reference denosumab (XGEVA® from European Union [EU‐reference] and United States [US‐reference]) in a healthy male population. The primary PK endpoints included: Area under the serum concentration versus time curve from time 0 to the last quantifiable concentration timepoint (AUC0–last); and maximum observed serum concentration (Cmax). Secondary endpoints included: AUC from time 0 extrapolated to infinity (AUC0–∞), time to reach maximum observed concentration, clearance, terminal phase half‐life, pharmacodynamic, safety, and immunogenicity assessments. A total of 255 subjects were randomized (1:1:1) to receive a subcutaneous 35 mg dose of MB09 or reference denosumab. Cmax was reached after denosumab administration, followed by a decline in the concentration with similar terminal phase half‐live across treatment arms. Systemic exposure of MB09 (AUC0–last and Cmax) was equivalent to the reference denosumab, as the 90% confidence intervals around the geometric least square mean ratios laid within the predefined acceptance limits (80.00%, 125.00%) across all comparisons. Pharmacodynamic parameters, based on the percent of change from baseline in serum C‐terminal telopeptide of Type 1 collagen levels, were similar across the three arms. The treatments were considered safe and generally well tolerated, with 92 treatment‐emergent adverse events reported (most Grade 2 and 3) and similarly distributed. Immunogenicity was low and similarly distributed. These results provide strong evidence that supports the biosimilarity between MB09 and denosumab reference products.
format Article
id doaj-art-57c1e8cb07884d4692bbf9f7180b3eae
institution OA Journals
issn 1752-8054
1752-8062
language English
publishDate 2024-09-01
publisher Wiley
record_format Article
series Clinical and Translational Science
spelling doaj-art-57c1e8cb07884d4692bbf9f7180b3eae2025-08-20T01:55:12ZengWileyClinical and Translational Science1752-80541752-80622024-09-01179n/an/a10.1111/cts.70013MB09, a denosumab biosimilar candidate: Biosimilarity demonstration in a phase I study in healthy subjectsMonika Tomaszewska‐Kiecana0Elisabete Carapuça1Amalia Florez‐Igual2Javier Queiruga‐Parada3Biokinetica S.A. Józefów PolandMedical Department mAbxience Research S.L.U. Madrid SpainMedical Department mAbxience Research S.L.U. Madrid SpainMedical Department mAbxience Research S.L.U. Madrid SpainAbstract This was a Phase I, randomized, double‐blinded, three‐arm, single‐dose, parallel study aimed to demonstrate pharmacokinetic (PK) similarity between MB09 (a denosumab biosimilar candidate) and reference denosumab (XGEVA® from European Union [EU‐reference] and United States [US‐reference]) in a healthy male population. The primary PK endpoints included: Area under the serum concentration versus time curve from time 0 to the last quantifiable concentration timepoint (AUC0–last); and maximum observed serum concentration (Cmax). Secondary endpoints included: AUC from time 0 extrapolated to infinity (AUC0–∞), time to reach maximum observed concentration, clearance, terminal phase half‐life, pharmacodynamic, safety, and immunogenicity assessments. A total of 255 subjects were randomized (1:1:1) to receive a subcutaneous 35 mg dose of MB09 or reference denosumab. Cmax was reached after denosumab administration, followed by a decline in the concentration with similar terminal phase half‐live across treatment arms. Systemic exposure of MB09 (AUC0–last and Cmax) was equivalent to the reference denosumab, as the 90% confidence intervals around the geometric least square mean ratios laid within the predefined acceptance limits (80.00%, 125.00%) across all comparisons. Pharmacodynamic parameters, based on the percent of change from baseline in serum C‐terminal telopeptide of Type 1 collagen levels, were similar across the three arms. The treatments were considered safe and generally well tolerated, with 92 treatment‐emergent adverse events reported (most Grade 2 and 3) and similarly distributed. Immunogenicity was low and similarly distributed. These results provide strong evidence that supports the biosimilarity between MB09 and denosumab reference products.https://doi.org/10.1111/cts.70013
spellingShingle Monika Tomaszewska‐Kiecana
Elisabete Carapuça
Amalia Florez‐Igual
Javier Queiruga‐Parada
MB09, a denosumab biosimilar candidate: Biosimilarity demonstration in a phase I study in healthy subjects
Clinical and Translational Science
title MB09, a denosumab biosimilar candidate: Biosimilarity demonstration in a phase I study in healthy subjects
title_full MB09, a denosumab biosimilar candidate: Biosimilarity demonstration in a phase I study in healthy subjects
title_fullStr MB09, a denosumab biosimilar candidate: Biosimilarity demonstration in a phase I study in healthy subjects
title_full_unstemmed MB09, a denosumab biosimilar candidate: Biosimilarity demonstration in a phase I study in healthy subjects
title_short MB09, a denosumab biosimilar candidate: Biosimilarity demonstration in a phase I study in healthy subjects
title_sort mb09 a denosumab biosimilar candidate biosimilarity demonstration in a phase i study in healthy subjects
url https://doi.org/10.1111/cts.70013
work_keys_str_mv AT monikatomaszewskakiecana mb09adenosumabbiosimilarcandidatebiosimilaritydemonstrationinaphaseistudyinhealthysubjects
AT elisabetecarapuca mb09adenosumabbiosimilarcandidatebiosimilaritydemonstrationinaphaseistudyinhealthysubjects
AT amaliaflorezigual mb09adenosumabbiosimilarcandidatebiosimilaritydemonstrationinaphaseistudyinhealthysubjects
AT javierqueirugaparada mb09adenosumabbiosimilarcandidatebiosimilaritydemonstrationinaphaseistudyinhealthysubjects