Biosynthesis and investigation of antibacterial properties of green silver nanoparticles using fruit extracts of Wild barberry, Medlar (Mespilus germanica L.), and Hawthorn

The biological method of synthesizing nanoparticles is a valuable alternative to traditional chemical synthesis methods, offering a fast, environmentally friendly, and cost-effective technique. In this study, the synthesis of silver nanoparticles (AgNPs) was carried out using fruit extracts of Wild...

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Main Authors: Hassan Koohestani, Maryam Nabilu, Ali Balooch
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:Food Chemistry Advances
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772753X24002454
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author Hassan Koohestani
Maryam Nabilu
Ali Balooch
author_facet Hassan Koohestani
Maryam Nabilu
Ali Balooch
author_sort Hassan Koohestani
collection DOAJ
description The biological method of synthesizing nanoparticles is a valuable alternative to traditional chemical synthesis methods, offering a fast, environmentally friendly, and cost-effective technique. In this study, the synthesis of silver nanoparticles (AgNPs) was carried out using fruit extracts of Wild barberry (Bar-Ag), Medlar (Mespilus germanica L.) (Med-Ag), and Hawthorn (Haw-Ag) as reducing and stabilizing agents. AgNPs were characterized by various analysis methods, including X-ray diffraction (XRD), UV–vis spectroscopy, Field Emission Scanning Electron Microscopy (FESEM), Transmission Electron Microscopy (TEM), and Dynamic Light Diffraction (DLS). The XRD results showed the presence of pure silver crystals with average sizes of 35.6, 23.7, and 35.6 nm for Bar-Ag, Med-Ag, and Haw-Ag, respectively. FESEM and TEM images illustrated the formation of nearly spherical and stable particles with sizes ranging from 30 to 80 nm. In addition to confirming the particle sizes, the DLS result indicated their uniform distribution. FTIR analysis revealed that AgNPs contain three main groups of agents: phenols, carboxylic acids, and amines, which play a role in the synthesis of nanoparticles. Evaluating the antibacterial activity against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria showed that AgNPs synthesized with these fruit extracts exhibited significant antibacterial performance. The results of this study demonstrate that AgNPs biosynthesized with fruit extracts have considerable potential for biomedical applications.
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spelling doaj-art-57bdad1576f04fb4a5d60f4ecfc2abdd2025-08-20T02:52:20ZengElsevierFood Chemistry Advances2772-753X2025-03-01610085010.1016/j.focha.2024.100850Biosynthesis and investigation of antibacterial properties of green silver nanoparticles using fruit extracts of Wild barberry, Medlar (Mespilus germanica L.), and HawthornHassan Koohestani0Maryam Nabilu1Ali Balooch2Corresponding author.; Faculty of Materials and Metallurgical Engineering, Semnan University, Semnan, IranFaculty of Materials and Metallurgical Engineering, Semnan University, Semnan, IranFaculty of Materials and Metallurgical Engineering, Semnan University, Semnan, IranThe biological method of synthesizing nanoparticles is a valuable alternative to traditional chemical synthesis methods, offering a fast, environmentally friendly, and cost-effective technique. In this study, the synthesis of silver nanoparticles (AgNPs) was carried out using fruit extracts of Wild barberry (Bar-Ag), Medlar (Mespilus germanica L.) (Med-Ag), and Hawthorn (Haw-Ag) as reducing and stabilizing agents. AgNPs were characterized by various analysis methods, including X-ray diffraction (XRD), UV–vis spectroscopy, Field Emission Scanning Electron Microscopy (FESEM), Transmission Electron Microscopy (TEM), and Dynamic Light Diffraction (DLS). The XRD results showed the presence of pure silver crystals with average sizes of 35.6, 23.7, and 35.6 nm for Bar-Ag, Med-Ag, and Haw-Ag, respectively. FESEM and TEM images illustrated the formation of nearly spherical and stable particles with sizes ranging from 30 to 80 nm. In addition to confirming the particle sizes, the DLS result indicated their uniform distribution. FTIR analysis revealed that AgNPs contain three main groups of agents: phenols, carboxylic acids, and amines, which play a role in the synthesis of nanoparticles. Evaluating the antibacterial activity against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria showed that AgNPs synthesized with these fruit extracts exhibited significant antibacterial performance. The results of this study demonstrate that AgNPs biosynthesized with fruit extracts have considerable potential for biomedical applications.http://www.sciencedirect.com/science/article/pii/S2772753X24002454Wild barberryMedlar (Mespilus germanica L.)HawthornGreen synthesisBiosynthesis
spellingShingle Hassan Koohestani
Maryam Nabilu
Ali Balooch
Biosynthesis and investigation of antibacterial properties of green silver nanoparticles using fruit extracts of Wild barberry, Medlar (Mespilus germanica L.), and Hawthorn
Food Chemistry Advances
Wild barberry
Medlar (Mespilus germanica L.)
Hawthorn
Green synthesis
Biosynthesis
title Biosynthesis and investigation of antibacterial properties of green silver nanoparticles using fruit extracts of Wild barberry, Medlar (Mespilus germanica L.), and Hawthorn
title_full Biosynthesis and investigation of antibacterial properties of green silver nanoparticles using fruit extracts of Wild barberry, Medlar (Mespilus germanica L.), and Hawthorn
title_fullStr Biosynthesis and investigation of antibacterial properties of green silver nanoparticles using fruit extracts of Wild barberry, Medlar (Mespilus germanica L.), and Hawthorn
title_full_unstemmed Biosynthesis and investigation of antibacterial properties of green silver nanoparticles using fruit extracts of Wild barberry, Medlar (Mespilus germanica L.), and Hawthorn
title_short Biosynthesis and investigation of antibacterial properties of green silver nanoparticles using fruit extracts of Wild barberry, Medlar (Mespilus germanica L.), and Hawthorn
title_sort biosynthesis and investigation of antibacterial properties of green silver nanoparticles using fruit extracts of wild barberry medlar mespilus germanica l and hawthorn
topic Wild barberry
Medlar (Mespilus germanica L.)
Hawthorn
Green synthesis
Biosynthesis
url http://www.sciencedirect.com/science/article/pii/S2772753X24002454
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AT maryamnabilu biosynthesisandinvestigationofantibacterialpropertiesofgreensilvernanoparticlesusingfruitextractsofwildbarberrymedlarmespilusgermanicalandhawthorn
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