Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma
There is a lack of information about the methods used for bovine platelet-rich plasma (PRP)/platelet-rich gel (PRG) procurement, including information on platelet (PLT), white blood cell (WBC) in PRP, and growth factor release from PRG supernatants. The aims of this study were to compare and to corr...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2017/1950401 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850223991080353792 |
|---|---|
| author | Claudia M. Gutiérrez Catalina López Carlos E. Giraldo Jorge U. Carmona |
| author_facet | Claudia M. Gutiérrez Catalina López Carlos E. Giraldo Jorge U. Carmona |
| author_sort | Claudia M. Gutiérrez |
| collection | DOAJ |
| description | There is a lack of information about the methods used for bovine platelet-rich plasma (PRP)/platelet-rich gel (PRG) procurement, including information on platelet (PLT), white blood cell (WBC) in PRP, and growth factor release from PRG supernatants. The aims of this study were to compare and to correlate the PLT, WBC, transforming growth factor beta-1 (TGF-β1), and platelet-derived growth factor BB (PDGF-BB) concentrations in bovine whole blood, plasma, and four PRP layers and their respective PRG supernatants: A and B (obtained by a single centrifugation tube method at 720g/5 min) and C and D (obtained by a double centrifugation tube method, by using two centrifugation episodes at 720g/5 min). PLT and WBC counts were significantly higher in PRP-C, followed by whole blood, PRP-A, PRP-B, and PRP-D. TGF-β1 concentrations were significantly higher in PRG-B supernatants and its correspondent PRP-B lysate when compared to the other PRG supernatants and plasma. Supernatants from PRG-A, PRG-B, and PRG-D had equivalent TGF-β1 concentrations. PDGF-BB concentrations were not statistically different between the hemoderivatives. Significant Pearson correlations were noted between PLT counts and WBC counts (0.8) and between PLT counts and PLT distribution width (0.6). Further studies should be performed to assess the potential clinical applications of these PRPs. |
| format | Article |
| id | doaj-art-57bd5d1be52e4eb78e5fe237adcb6f65 |
| institution | OA Journals |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-57bd5d1be52e4eb78e5fe237adcb6f652025-08-20T02:05:46ZengWileyVeterinary Medicine International2090-81132042-00482017-01-01201710.1155/2017/19504011950401Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich PlasmaClaudia M. Gutiérrez0Catalina López1Carlos E. Giraldo2Jorge U. Carmona3Grupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, ColombiaGrupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, ColombiaGrupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, ColombiaGrupo de Investigación Terapia Regenerativa, Departamento de Salud Animal, Universidad de Caldas, Manizales, ColombiaThere is a lack of information about the methods used for bovine platelet-rich plasma (PRP)/platelet-rich gel (PRG) procurement, including information on platelet (PLT), white blood cell (WBC) in PRP, and growth factor release from PRG supernatants. The aims of this study were to compare and to correlate the PLT, WBC, transforming growth factor beta-1 (TGF-β1), and platelet-derived growth factor BB (PDGF-BB) concentrations in bovine whole blood, plasma, and four PRP layers and their respective PRG supernatants: A and B (obtained by a single centrifugation tube method at 720g/5 min) and C and D (obtained by a double centrifugation tube method, by using two centrifugation episodes at 720g/5 min). PLT and WBC counts were significantly higher in PRP-C, followed by whole blood, PRP-A, PRP-B, and PRP-D. TGF-β1 concentrations were significantly higher in PRG-B supernatants and its correspondent PRP-B lysate when compared to the other PRG supernatants and plasma. Supernatants from PRG-A, PRG-B, and PRG-D had equivalent TGF-β1 concentrations. PDGF-BB concentrations were not statistically different between the hemoderivatives. Significant Pearson correlations were noted between PLT counts and WBC counts (0.8) and between PLT counts and PLT distribution width (0.6). Further studies should be performed to assess the potential clinical applications of these PRPs.http://dx.doi.org/10.1155/2017/1950401 |
| spellingShingle | Claudia M. Gutiérrez Catalina López Carlos E. Giraldo Jorge U. Carmona Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma Veterinary Medicine International |
| title | Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma |
| title_full | Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma |
| title_fullStr | Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma |
| title_full_unstemmed | Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma |
| title_short | Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma |
| title_sort | study of a two step centrifugation protocol for concentrating cells and growth factors in bovine platelet rich plasma |
| url | http://dx.doi.org/10.1155/2017/1950401 |
| work_keys_str_mv | AT claudiamgutierrez studyofatwostepcentrifugationprotocolforconcentratingcellsandgrowthfactorsinbovineplateletrichplasma AT catalinalopez studyofatwostepcentrifugationprotocolforconcentratingcellsandgrowthfactorsinbovineplateletrichplasma AT carlosegiraldo studyofatwostepcentrifugationprotocolforconcentratingcellsandgrowthfactorsinbovineplateletrichplasma AT jorgeucarmona studyofatwostepcentrifugationprotocolforconcentratingcellsandgrowthfactorsinbovineplateletrichplasma |