Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus
Background: Accurate and rapid detection of methicillin-resistant Staphylococcus aureus is very important in a clinical laboratory setting to avoid treatment failure. Conventional methods were compared against the gold standard polymerase chain reaction (PCR) technique to determine the best combinat...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2008-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/321 |
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| author | Riza Adaleti Yasar Nakipoglu Zeynep Ceren Karahan Cihan Tasdemir Fatma Kaya |
| author_facet | Riza Adaleti Yasar Nakipoglu Zeynep Ceren Karahan Cihan Tasdemir Fatma Kaya |
| author_sort | Riza Adaleti |
| collection | DOAJ |
| description | Background: Accurate and rapid detection of methicillin-resistant Staphylococcus aureus is very important in a clinical laboratory setting to avoid treatment failure. Conventional methods were compared against the gold standard polymerase chain reaction (PCR) technique to determine the best combination of the routine procedures.
Methodology: Methicillin resistance was investigated in 416 clinical Staphylococcus aureus isolates by PCR, oxacillin agar screening (OAS), oxacillin disk diffusion (ODD) and cefoxitin disk diffusion (CDD) methods.
Results: Two hundred and ten (51%) out of 416 S. aureus strains were found to be mecA-positive by PCR. Sensitivity and specificity of the ODD, CDD and OAS methods were detected as follows: 100% and 89%, 99.50% and 100%, and 99.50% and 100%, respectively.
Conclusion: Combining the ODD and CDD methods could be a good choice for detecting methicillin resistance in S. aureus strains where mecA PCR cannot be performed. |
| format | Article |
| id | doaj-art-57bae272f7c54e618ca2fd00625ee733 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2008-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-57bae272f7c54e618ca2fd00625ee7332025-08-20T03:48:58ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802008-02-0120110.3855/jidc.321Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureusRiza Adaleti0Yasar Nakipoglu1Zeynep Ceren Karahan2Cihan Tasdemir3Fatma Kaya4Clinical Microbiology Laboratory, Haydarpasa Numune Education and Research Hospital, IstanbulDepartment of Microbiology and Clinical Microbiology, Faculty of Medicine, University of Istanbul, IstanbulDepartment of Microbiology and Clinical Microbiology, Faculty of Medicine, Ankara University, AnkaraClinical Microbiology Laboratory, Haydarpasa Numune Education and Research Hospital, IstanbulClinical Microbiology Laboratory, Haydarpasa Numune Education and Research Hospital, IstanbulBackground: Accurate and rapid detection of methicillin-resistant Staphylococcus aureus is very important in a clinical laboratory setting to avoid treatment failure. Conventional methods were compared against the gold standard polymerase chain reaction (PCR) technique to determine the best combination of the routine procedures. Methodology: Methicillin resistance was investigated in 416 clinical Staphylococcus aureus isolates by PCR, oxacillin agar screening (OAS), oxacillin disk diffusion (ODD) and cefoxitin disk diffusion (CDD) methods. Results: Two hundred and ten (51%) out of 416 S. aureus strains were found to be mecA-positive by PCR. Sensitivity and specificity of the ODD, CDD and OAS methods were detected as follows: 100% and 89%, 99.50% and 100%, and 99.50% and 100%, respectively. Conclusion: Combining the ODD and CDD methods could be a good choice for detecting methicillin resistance in S. aureus strains where mecA PCR cannot be performed.https://jidc.org/index.php/journal/article/view/321Methicillin-resistant Staphylococcus aureus (MRSA)polymerase chain reaction (PCR)oxacillin disk diffusioncefoxitin disk diffusionoxacillin agar screening |
| spellingShingle | Riza Adaleti Yasar Nakipoglu Zeynep Ceren Karahan Cihan Tasdemir Fatma Kaya Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus Journal of Infection in Developing Countries Methicillin-resistant Staphylococcus aureus (MRSA) polymerase chain reaction (PCR) oxacillin disk diffusion cefoxitin disk diffusion oxacillin agar screening |
| title | Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus |
| title_full | Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus |
| title_fullStr | Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus |
| title_full_unstemmed | Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus |
| title_short | Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus |
| title_sort | comparison of polymerase chain reaction and conventional methods in detecting methicillin resistant staphylococcus aureus |
| topic | Methicillin-resistant Staphylococcus aureus (MRSA) polymerase chain reaction (PCR) oxacillin disk diffusion cefoxitin disk diffusion oxacillin agar screening |
| url | https://jidc.org/index.php/journal/article/view/321 |
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