Tyrosine Hydroxylase Expression in Differentiating Neurons of the Rat Arcuate Nucleus: Stimulatory Influence of Serotonin Afferents
The influence of serotonin afferents on tyrosine hydroxylase expression in differentiating neurons of the rat arcuate nucleus was studied in vivo and in vitro. In the in vivo study, pchlorophenylalanine inhibited serotonin synthesis in fetal brain from the 11th to the 20th embryonic day. We then u...
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| Format: | Article |
| Language: | English |
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Wiley
2001-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/NP.2001.271 |
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| author | Viktoria I. Melnikova Michael V. Ugrumov Evgenia V. Proshlyakova André Calas Jean Thibault |
| author_facet | Viktoria I. Melnikova Michael V. Ugrumov Evgenia V. Proshlyakova André Calas Jean Thibault |
| author_sort | Viktoria I. Melnikova |
| collection | DOAJ |
| description | The influence of serotonin afferents on
tyrosine hydroxylase expression in differentiating
neurons of the rat arcuate nucleus was studied
in vivo and in vitro. In the in vivo study, pchlorophenylalanine
inhibited serotonin synthesis
in fetal brain from the 11th
to the 20th
embryonic
day. We then used semiquantitative immunocytochemistry
to evaluate tyrosine hydroxylase
levels in neurons of the arcuate nucleus in
fetuses at the 21st
embryonic day or in offspring
at the 35th
postnatal day. Serotonin depltion
significantly decreased the tyrosine hydroxylase
content in neurons of males and females at the
21st
embryonic day and in males at the 35th
postnatal day. For the in vitro study, embryonic
neurons of the arcuate nucleus were cocultured
with embryonic neurons of the raphe
nucleus, the main source of serotonin innervation
of the brain, including the arcuate nucleus.
Co-culture of the neurons resulted in a genderspecific
increase of the tyrosine hydroxylase
level in the neurons of the arcuate nucleus. In
turn, the neurons of the raphe nucleus showed
increased levels .of serotonin in both males and
females, with no sexual dimorphism. Thus, our
results suggest a stimulatory, long-lasting effect
of serotonin afferents on tyrosine hydroxylase
expression in the differentiating neurons of the
rat arcuate nucleus during prenatal ontogenesis. |
| format | Article |
| id | doaj-art-57b7c08fcdcf4dfc958a742c2ce6082e |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2001-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-57b7c08fcdcf4dfc958a742c2ce6082e2025-02-03T01:23:47ZengWileyNeural Plasticity2090-59041687-54432001-01-018427128410.1155/NP.2001.271Tyrosine Hydroxylase Expression in Differentiating Neurons of the Rat Arcuate Nucleus: Stimulatory Influence of Serotonin AfferentsViktoria I. Melnikova0Michael V. Ugrumov1Evgenia V. Proshlyakova2André Calas3Jean Thibault4Laboratory of Hormonal Regulations, Institute of Developmental Biology, Russian Academy of Medical Sciences, Moscow, RussiaLaboratory of Hormonal Regulations, Institute of Developmental Biology, Russian Academy of Medical Sciences, Moscow, RussiaLaboratory of Hormonal Regulations, Institute of Developmental Biology, Russian Academy of Medical Sciences, Moscow, RussiaDépartement des Signaux Intercellulaires, Institut des Neurosciences, Université P. et M. Curie, paris, FranceLaboratoire de Neurobiologie Moléculaire, Université Paris, Créteil, FranceThe influence of serotonin afferents on tyrosine hydroxylase expression in differentiating neurons of the rat arcuate nucleus was studied in vivo and in vitro. In the in vivo study, pchlorophenylalanine inhibited serotonin synthesis in fetal brain from the 11th to the 20th embryonic day. We then used semiquantitative immunocytochemistry to evaluate tyrosine hydroxylase levels in neurons of the arcuate nucleus in fetuses at the 21st embryonic day or in offspring at the 35th postnatal day. Serotonin depltion significantly decreased the tyrosine hydroxylase content in neurons of males and females at the 21st embryonic day and in males at the 35th postnatal day. For the in vitro study, embryonic neurons of the arcuate nucleus were cocultured with embryonic neurons of the raphe nucleus, the main source of serotonin innervation of the brain, including the arcuate nucleus. Co-culture of the neurons resulted in a genderspecific increase of the tyrosine hydroxylase level in the neurons of the arcuate nucleus. In turn, the neurons of the raphe nucleus showed increased levels .of serotonin in both males and females, with no sexual dimorphism. Thus, our results suggest a stimulatory, long-lasting effect of serotonin afferents on tyrosine hydroxylase expression in the differentiating neurons of the rat arcuate nucleus during prenatal ontogenesis.http://dx.doi.org/10.1155/NP.2001.271 |
| spellingShingle | Viktoria I. Melnikova Michael V. Ugrumov Evgenia V. Proshlyakova André Calas Jean Thibault Tyrosine Hydroxylase Expression in Differentiating Neurons of the Rat Arcuate Nucleus: Stimulatory Influence of Serotonin Afferents Neural Plasticity |
| title | Tyrosine Hydroxylase Expression in Differentiating Neurons of the
Rat Arcuate Nucleus: Stimulatory Influence of Serotonin Afferents |
| title_full | Tyrosine Hydroxylase Expression in Differentiating Neurons of the
Rat Arcuate Nucleus: Stimulatory Influence of Serotonin Afferents |
| title_fullStr | Tyrosine Hydroxylase Expression in Differentiating Neurons of the
Rat Arcuate Nucleus: Stimulatory Influence of Serotonin Afferents |
| title_full_unstemmed | Tyrosine Hydroxylase Expression in Differentiating Neurons of the
Rat Arcuate Nucleus: Stimulatory Influence of Serotonin Afferents |
| title_short | Tyrosine Hydroxylase Expression in Differentiating Neurons of the
Rat Arcuate Nucleus: Stimulatory Influence of Serotonin Afferents |
| title_sort | tyrosine hydroxylase expression in differentiating neurons of the rat arcuate nucleus stimulatory influence of serotonin afferents |
| url | http://dx.doi.org/10.1155/NP.2001.271 |
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