Rutin attenuates zearalenone-induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathway
Zearalenone (ZEA) is an environmentally widespread mycotoxin capable of posing a serious threat to food safety and public health, and porcine endometrial stromal cells (ESCs) are particularly sensitive to the toxic effects of ZEA. We hypothesized that Rutin, a flavonoid antioxidant, could significan...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324016221 |
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| author | Chenlong Wang Chuangjiang Chen MengYa Wang Sajid Ur Rahman Bin Wei Hongyan Ding Wanyue Huang Xichun Wang |
| author_facet | Chenlong Wang Chuangjiang Chen MengYa Wang Sajid Ur Rahman Bin Wei Hongyan Ding Wanyue Huang Xichun Wang |
| author_sort | Chenlong Wang |
| collection | DOAJ |
| description | Zearalenone (ZEA) is an environmentally widespread mycotoxin capable of posing a serious threat to food safety and public health, and porcine endometrial stromal cells (ESCs) are particularly sensitive to the toxic effects of ZEA. We hypothesized that Rutin, a flavonoid antioxidant, could significantly alleviate ZEA-induced ferroptosis through the p53 signaling pathway. In this study, we used porcine ESCs as a research model. When porcine ESCs were co-cultured with the addition of Rutin and ZEA following p53 gene silencing via siRNA transfection, Rutin significantly mitigated ZEA-induced mitochondrial damage, oxidative stress, and Fe2 + content through the p53 pathway. Additionally Rutin lowered the expression of p53, ALOX12, and ACSL4 while significantly improving cytokinesis, antioxidant enzyme activity, and SLC7A11, GPX4, Nrf2, FTH1, thereby inhibiting cellular ferroptosis. These findings suggested a novel programmed death mechanism for alleviating the cytotoxic effects of ZEA, involving the knockdown of p53. |
| format | Article |
| id | doaj-art-579fa45ad4e64d69aaa91ab9dac8e14a |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-579fa45ad4e64d69aaa91ab9dac8e14a2025-02-12T05:29:37ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117546Rutin attenuates zearalenone-induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathwayChenlong Wang0Chuangjiang Chen1MengYa Wang2Sajid Ur Rahman3Bin Wei4Hongyan Ding5Wanyue Huang6Xichun Wang7College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, ChinaCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, ChinaCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, ChinaCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, ChinaHuangyuan County Animal Husbandry and Veterinary Station, Xining, Qinghai 812100, ChinaAnhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering, Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, ChinaCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China; Anhui Province Engineering Laboratory for Animal Food Quality and Bio-safety, Hefei 230036, China; Correspondence to: College of Veterinary Medicine, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, China.Zearalenone (ZEA) is an environmentally widespread mycotoxin capable of posing a serious threat to food safety and public health, and porcine endometrial stromal cells (ESCs) are particularly sensitive to the toxic effects of ZEA. We hypothesized that Rutin, a flavonoid antioxidant, could significantly alleviate ZEA-induced ferroptosis through the p53 signaling pathway. In this study, we used porcine ESCs as a research model. When porcine ESCs were co-cultured with the addition of Rutin and ZEA following p53 gene silencing via siRNA transfection, Rutin significantly mitigated ZEA-induced mitochondrial damage, oxidative stress, and Fe2 + content through the p53 pathway. Additionally Rutin lowered the expression of p53, ALOX12, and ACSL4 while significantly improving cytokinesis, antioxidant enzyme activity, and SLC7A11, GPX4, Nrf2, FTH1, thereby inhibiting cellular ferroptosis. These findings suggested a novel programmed death mechanism for alleviating the cytotoxic effects of ZEA, involving the knockdown of p53.http://www.sciencedirect.com/science/article/pii/S0147651324016221ZEARutinESCsFerroptosisp53 signaling pathway |
| spellingShingle | Chenlong Wang Chuangjiang Chen MengYa Wang Sajid Ur Rahman Bin Wei Hongyan Ding Wanyue Huang Xichun Wang Rutin attenuates zearalenone-induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathway Ecotoxicology and Environmental Safety ZEA Rutin ESCs Ferroptosis p53 signaling pathway |
| title | Rutin attenuates zearalenone-induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathway |
| title_full | Rutin attenuates zearalenone-induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathway |
| title_fullStr | Rutin attenuates zearalenone-induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathway |
| title_full_unstemmed | Rutin attenuates zearalenone-induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathway |
| title_short | Rutin attenuates zearalenone-induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathway |
| title_sort | rutin attenuates zearalenone induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathway |
| topic | ZEA Rutin ESCs Ferroptosis p53 signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324016221 |
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