In Vivo Antiplasmodial Activity of Entandrophragma cylindricum (Sprague) Sprague Ethyl Acetate Extract in Plasmodium berghei-Infected Mice
Background. One of the most dangerous Plasmodium species is Plasmodium falciparum. Hence, it causes a higher rate of mortality. The resistance of Plasmodium falciparum to the ACT (Artemisinin-based Combination Therapies) has led to the search for new antimalarial drugs. The purpose of this research...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/8846067 |
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| author | Noumedem Anangmo Christelle Nadia Yamssi Cédric Ngongang Ouankou Christian Simeni Njonnou Sylvain Raoul Yondo Jeanette Mounvera Abdel Azizi Sop Foka Eric Igor Djape Guangue Diane Chahdini Gbambie Abass Tsila Henri Gabriel Mpoame Mbida |
| author_facet | Noumedem Anangmo Christelle Nadia Yamssi Cédric Ngongang Ouankou Christian Simeni Njonnou Sylvain Raoul Yondo Jeanette Mounvera Abdel Azizi Sop Foka Eric Igor Djape Guangue Diane Chahdini Gbambie Abass Tsila Henri Gabriel Mpoame Mbida |
| author_sort | Noumedem Anangmo Christelle Nadia |
| collection | DOAJ |
| description | Background. One of the most dangerous Plasmodium species is Plasmodium falciparum. Hence, it causes a higher rate of mortality. The resistance of Plasmodium falciparum to the ACT (Artemisinin-based Combination Therapies) has led to the search for new antimalarial drugs. The purpose of this research was to assess the in vivo antiplasmodial activity of Entandrophragma cylindricum ethyl acetate extract to provide a scientific basis for the use of this medicinal plant to treat malaria. Methods. Entandrophragma cylindricum stem bark powder was macerated in ethyl acetate to obtain the extract. The extract liquid filtrate was concentrated, evaporated and dry using a Rotavapor. The Peter and Rane test were used for the suppressive and curative antiplasmodial activities at different doses (125, 250 and 500 mg/kg). A positive and negative control groups were administered chloroquine (5 mg/kg) and 10% hypromelose, respectively. To assess the parasitemia of the mice a thin blood smear was made. Results. The ethyl acetate extract completely (100%) inhibited the development of P. berghei in the suppressive test at the dose of 500 mg/kg while that of the curative test was inhibited at 95%. The extract-treated group (500 mg/kg) and (Chloroquine (5 mg/kg) group all survived. The negative control group recorded a 100% mortality rate. Conclusion. The present study provides scientific confirmation on the use of E. cylindricum stem bark as an antiplasmodial remedy. However, the identification of the mode of action and the purification of the active compounds are necessary for further studies. |
| format | Article |
| id | doaj-art-57940ae581ba4f13b3c9a2fcb24ec02e |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-57940ae581ba4f13b3c9a2fcb24ec02e2025-08-20T03:33:36ZengWileyJournal of Parasitology Research2090-00232090-00312020-01-01202010.1155/2020/88460678846067In Vivo Antiplasmodial Activity of Entandrophragma cylindricum (Sprague) Sprague Ethyl Acetate Extract in Plasmodium berghei-Infected MiceNoumedem Anangmo Christelle Nadia0Yamssi Cédric1Ngongang Ouankou Christian2Simeni Njonnou Sylvain Raoul3Yondo Jeanette4Mounvera Abdel Azizi5Sop Foka Eric Igor6Djape Guangue Diane7Chahdini Gbambie Abass8Tsila Henri Gabriel9Mpoame Mbida10Department of Microbiology, Haematology and Immunology Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, P.O. Box 96, Dschang, CameroonDepartment of Biomedical Sciences, Faculty of Health Sciences, University of Bamenda, P.O. Box 39 Bambili, CameroonDepartment of Internal Medicine and Specialties, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, P.O. Box 96, Dschang, CameroonDepartment of Internal Medicine and Specialties, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, P.O. Box 96, Dschang, CameroonDepartment of Microbiology, Haematology and Immunology Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, P.O. Box 96, Dschang, CameroonDepartment of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, CameroonDepartment of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, CameroonDepartment of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, CameroonDepartment of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, CameroonDepartment of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, CameroonDepartment of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, CameroonBackground. One of the most dangerous Plasmodium species is Plasmodium falciparum. Hence, it causes a higher rate of mortality. The resistance of Plasmodium falciparum to the ACT (Artemisinin-based Combination Therapies) has led to the search for new antimalarial drugs. The purpose of this research was to assess the in vivo antiplasmodial activity of Entandrophragma cylindricum ethyl acetate extract to provide a scientific basis for the use of this medicinal plant to treat malaria. Methods. Entandrophragma cylindricum stem bark powder was macerated in ethyl acetate to obtain the extract. The extract liquid filtrate was concentrated, evaporated and dry using a Rotavapor. The Peter and Rane test were used for the suppressive and curative antiplasmodial activities at different doses (125, 250 and 500 mg/kg). A positive and negative control groups were administered chloroquine (5 mg/kg) and 10% hypromelose, respectively. To assess the parasitemia of the mice a thin blood smear was made. Results. The ethyl acetate extract completely (100%) inhibited the development of P. berghei in the suppressive test at the dose of 500 mg/kg while that of the curative test was inhibited at 95%. The extract-treated group (500 mg/kg) and (Chloroquine (5 mg/kg) group all survived. The negative control group recorded a 100% mortality rate. Conclusion. The present study provides scientific confirmation on the use of E. cylindricum stem bark as an antiplasmodial remedy. However, the identification of the mode of action and the purification of the active compounds are necessary for further studies.http://dx.doi.org/10.1155/2020/8846067 |
| spellingShingle | Noumedem Anangmo Christelle Nadia Yamssi Cédric Ngongang Ouankou Christian Simeni Njonnou Sylvain Raoul Yondo Jeanette Mounvera Abdel Azizi Sop Foka Eric Igor Djape Guangue Diane Chahdini Gbambie Abass Tsila Henri Gabriel Mpoame Mbida In Vivo Antiplasmodial Activity of Entandrophragma cylindricum (Sprague) Sprague Ethyl Acetate Extract in Plasmodium berghei-Infected Mice Journal of Parasitology Research |
| title | In Vivo Antiplasmodial Activity of Entandrophragma cylindricum (Sprague) Sprague Ethyl Acetate Extract in Plasmodium berghei-Infected Mice |
| title_full | In Vivo Antiplasmodial Activity of Entandrophragma cylindricum (Sprague) Sprague Ethyl Acetate Extract in Plasmodium berghei-Infected Mice |
| title_fullStr | In Vivo Antiplasmodial Activity of Entandrophragma cylindricum (Sprague) Sprague Ethyl Acetate Extract in Plasmodium berghei-Infected Mice |
| title_full_unstemmed | In Vivo Antiplasmodial Activity of Entandrophragma cylindricum (Sprague) Sprague Ethyl Acetate Extract in Plasmodium berghei-Infected Mice |
| title_short | In Vivo Antiplasmodial Activity of Entandrophragma cylindricum (Sprague) Sprague Ethyl Acetate Extract in Plasmodium berghei-Infected Mice |
| title_sort | in vivo antiplasmodial activity of entandrophragma cylindricum sprague sprague ethyl acetate extract in plasmodium berghei infected mice |
| url | http://dx.doi.org/10.1155/2020/8846067 |
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