<i>Pijolavirus UFJF_PfSW6</i> Infection in <i>Pseudomonas fluorescens</i> Induces a Prophage Belonging to a Novel Genus in <i>Peduoviridae</i> Family
Background/Objectives: This study explores the genome sequencing data from the infection of <i>Pseudomonas fluorescens</i> UFV 041 by the bacteriophage <i>Pijolavirus UFJF_PfSW6</i>, aiming to identify and characterize prophages induced in the host bacterium during the infect...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | DNA |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-8856/4/4/35 |
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| Summary: | Background/Objectives: This study explores the genome sequencing data from the infection of <i>Pseudomonas fluorescens</i> UFV 041 by the bacteriophage <i>Pijolavirus UFJF_PfSW6</i>, aiming to identify and characterize prophages induced in the host bacterium during the infection. Methods: Scaffolds from sequencing data were analyzed, and reads were mapped to identify potential prophages using phage-to-host coverage metrics. The putative prophage scaffold was annotated, taxonomically classified, and its integration in the host bacterium was verified by PCR amplification of two target genes. We also tested whether mitomycin treatment could induce the prophage to enter the lytic cycle. Results: The prophage <i>UFJF_PfPro</i> was identified with a high phage-to-host coverage ratio. Its genome is 32,700 bp in length, containing 42 genes, 3 terminators, and 11 promoters, with 98.84% completeness. PCR confirmed its integration into <i>P. fluorescens</i> UFV 041, but mitomycin treatment did not induce the lytic cycle. The <i>UFJF_PfPro</i> genome shares 38.60% similarity with the closest lytic phages in the <i>Phitrevirus</i> genus, below genus and species assignment thresholds. A viral proteomic tree clustered <i>UFJF_PfPro</i> with <i>Phitrevirus</i> in a clade representing the <i>Peduoviridae</i> family. Conclusions: The <i>UFJF_PfPro</i> is a prophage integrated into the <i>P. fluorescens</i> UFV 041 genome, but we were unable to induce it to enter the lytic cycle using mitomycin treatment. The genome of <i>UFJF_PfPro</i> encodes all structural proteins typical of the <i>Caudoviricetes</i> class and shares low genomic similarity with species of the genus <i>Phitrevirus</i>, suggesting that <i>UFJF_PfPro</i> represents a new genus and species within the <i>Peduoviridae</i> family. |
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| ISSN: | 2673-8856 |