Molecular epidemiological characterization of human bocavirus (HBoV) in acute respiratory infection (ARI) patients in Yucheng, China

ObjectiveTo investigate the epidemiological characteristics and genetic profile of human bocavirus (HBoV) among patients with acute respiratory infection (ARI) in Yucheng, Henan, China.MethodsA total of 1,153 throat swabs were collected from ARI patients between March and June 2023. These samples we...

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Main Authors: Qi Wen, Ronghua Yang, Qin Luo, Qiangqiang Shi, Ze Chen, Chen Gao, Haijun Du, Guoyong Mei, Shuying Li, QinQin Song, Jun Han
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Public Health
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Online Access:https://www.frontiersin.org/articles/10.3389/fpubh.2025.1548907/full
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author Qi Wen
Qi Wen
Ronghua Yang
Qin Luo
Qiangqiang Shi
Ze Chen
Chen Gao
Haijun Du
Guoyong Mei
Shuying Li
QinQin Song
Jun Han
author_facet Qi Wen
Qi Wen
Ronghua Yang
Qin Luo
Qiangqiang Shi
Ze Chen
Chen Gao
Haijun Du
Guoyong Mei
Shuying Li
QinQin Song
Jun Han
author_sort Qi Wen
collection DOAJ
description ObjectiveTo investigate the epidemiological characteristics and genetic profile of human bocavirus (HBoV) among patients with acute respiratory infection (ARI) in Yucheng, Henan, China.MethodsA total of 1,153 throat swabs were collected from ARI patients between March and June 2023. These samples were tested for 18 pathogens using quantitative chain reaction (qPCR). The VP1 gene were amplified and sequenced from HBoV-positive samples. The viral genetic characterization was analyzed by comparing the HBoV1 sequences with reference sequences.ResultsOf the 1,153 samples, the detection rate of HBoV positive was 6.85%, primarily detected in May 2023. The majority of HBoV positive cases were found in children under 5 years old. Clinical manifestations in HBoV-positive patients predominantly were fever and cough, with a clinical diagnosis of lower respiratory tract infection. Other viruses were also detected in 20% of HBoV-positive samples. All HBoV sequences identified in this study belonged to HBoV1, with the Ib sublineage being the predominant strain circulating in Yucheng during March–June 2023.ConclusionAfter the implementation of the optimized COVID-19 prevention and control strategy in December 2022, HBoV infection was prevalent in Yucheng, Henan in May-June 2023, mainly among children younger than 5 years old, especially those under 2 years old. The Ib sublineage of HBoV was the dominant circulating strain in Yucheng, Henan during March–June 2023.
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spelling doaj-art-578ec886e7a140aa9828bfeac7c64fdd2025-08-20T02:26:10ZengFrontiers Media S.A.Frontiers in Public Health2296-25652025-04-011310.3389/fpubh.2025.15489071548907Molecular epidemiological characterization of human bocavirus (HBoV) in acute respiratory infection (ARI) patients in Yucheng, ChinaQi Wen0Qi Wen1Ronghua Yang2Qin Luo3Qiangqiang Shi4Ze Chen5Chen Gao6Haijun Du7Guoyong Mei8Shuying Li9QinQin Song10Jun Han11National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaHebei Key Laboratory for Chronic Disease, College of Basic Medicine, North China University of Science and Technology, Tangshan, ChinaYucheng Center for Disease Control and Prevention, Shangqiu, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaHebei Key Laboratory for Chronic Disease, College of Basic Medicine, North China University of Science and Technology, Tangshan, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaObjectiveTo investigate the epidemiological characteristics and genetic profile of human bocavirus (HBoV) among patients with acute respiratory infection (ARI) in Yucheng, Henan, China.MethodsA total of 1,153 throat swabs were collected from ARI patients between March and June 2023. These samples were tested for 18 pathogens using quantitative chain reaction (qPCR). The VP1 gene were amplified and sequenced from HBoV-positive samples. The viral genetic characterization was analyzed by comparing the HBoV1 sequences with reference sequences.ResultsOf the 1,153 samples, the detection rate of HBoV positive was 6.85%, primarily detected in May 2023. The majority of HBoV positive cases were found in children under 5 years old. Clinical manifestations in HBoV-positive patients predominantly were fever and cough, with a clinical diagnosis of lower respiratory tract infection. Other viruses were also detected in 20% of HBoV-positive samples. All HBoV sequences identified in this study belonged to HBoV1, with the Ib sublineage being the predominant strain circulating in Yucheng during March–June 2023.ConclusionAfter the implementation of the optimized COVID-19 prevention and control strategy in December 2022, HBoV infection was prevalent in Yucheng, Henan in May-June 2023, mainly among children younger than 5 years old, especially those under 2 years old. The Ib sublineage of HBoV was the dominant circulating strain in Yucheng, Henan during March–June 2023.https://www.frontiersin.org/articles/10.3389/fpubh.2025.1548907/fullacute respiratory infectionhuman bocavirusepidemiologygenetic characteristicsamino acid mutationclinical characteristics
spellingShingle Qi Wen
Qi Wen
Ronghua Yang
Qin Luo
Qiangqiang Shi
Ze Chen
Chen Gao
Haijun Du
Guoyong Mei
Shuying Li
QinQin Song
Jun Han
Molecular epidemiological characterization of human bocavirus (HBoV) in acute respiratory infection (ARI) patients in Yucheng, China
Frontiers in Public Health
acute respiratory infection
human bocavirus
epidemiology
genetic characteristics
amino acid mutation
clinical characteristics
title Molecular epidemiological characterization of human bocavirus (HBoV) in acute respiratory infection (ARI) patients in Yucheng, China
title_full Molecular epidemiological characterization of human bocavirus (HBoV) in acute respiratory infection (ARI) patients in Yucheng, China
title_fullStr Molecular epidemiological characterization of human bocavirus (HBoV) in acute respiratory infection (ARI) patients in Yucheng, China
title_full_unstemmed Molecular epidemiological characterization of human bocavirus (HBoV) in acute respiratory infection (ARI) patients in Yucheng, China
title_short Molecular epidemiological characterization of human bocavirus (HBoV) in acute respiratory infection (ARI) patients in Yucheng, China
title_sort molecular epidemiological characterization of human bocavirus hbov in acute respiratory infection ari patients in yucheng china
topic acute respiratory infection
human bocavirus
epidemiology
genetic characteristics
amino acid mutation
clinical characteristics
url https://www.frontiersin.org/articles/10.3389/fpubh.2025.1548907/full
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