Impact of dietary enzyme and fermentation treatments on the performance, digestibility, and biochemical profile of broilers consuming guar meal

This study aimed to evaluate the effects of enzyme treatment and fermentation of guar meal on growth performance, nutrient digestibility, and blood biochemistry in broiler chickens. A total of 900 day-old male Hubbard broiler chicks were divided into nine groups based on three levels of guar meal in...

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Main Authors: Abdul Hafeez, Muhammad Haris, Shabana Naz, Abdulwahed Fahad Alrefaei, Rifat Ullah Khan, Naseer Khan Momand, Demilade Israel Ibiwoye
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Italian Journal of Animal Science
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Online Access:http://dx.doi.org/10.1080/1828051X.2025.2484442
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author Abdul Hafeez
Muhammad Haris
Shabana Naz
Abdulwahed Fahad Alrefaei
Rifat Ullah Khan
Naseer Khan Momand
Demilade Israel Ibiwoye
author_facet Abdul Hafeez
Muhammad Haris
Shabana Naz
Abdulwahed Fahad Alrefaei
Rifat Ullah Khan
Naseer Khan Momand
Demilade Israel Ibiwoye
author_sort Abdul Hafeez
collection DOAJ
description This study aimed to evaluate the effects of enzyme treatment and fermentation of guar meal on growth performance, nutrient digestibility, and blood biochemistry in broiler chickens. A total of 900 day-old male Hubbard broiler chicks were divided into nine groups based on three levels of guar meal inclusion (3%, 6% and 9%) treated with enzymes (phytase, protease and β-mannanase) or fermented with Bacillus subtilis. The experiment followed a 3 × 3 factorial design, and growth parameters, nutrient digestibility, and blood biochemical analysis were conducted over a 21–day period. Significant (p < 0.05) improvements in feed intake and weight gain were observed in the enzyme-treated and fermented groups compared to untreated controls, particularly at 3% and 6% guar meal levels. Nutrient digestibility was higher for crude protein (p < 0.05) and calcium (p < 0.05) in enzyme-treated and fermented diets, with 3% and 6% inclusion levels showing better digestibility. Blood biochemical analysis indicated lower LDL (p < 0.05) in the fermented group and significant variations in triglycerides (p < 0.05) and HDL (p < 0.05) levels with different inclusion rates of guar meal. These findings suggest that enzyme treatment and fermentation of guar meal can enhance broiler growth performance, nutrient utilisation, and certain blood parameters, with the optimal effect seen at 3% and 6% inclusion levels.
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spelling doaj-art-577f8ed879df4d8196a430b8ca7ca0df2025-08-20T02:08:54ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2025-12-0124188589310.1080/1828051X.2025.24844422484442Impact of dietary enzyme and fermentation treatments on the performance, digestibility, and biochemical profile of broilers consuming guar mealAbdul Hafeez0Muhammad Haris1Shabana Naz2Abdulwahed Fahad Alrefaei3Rifat Ullah Khan4Naseer Khan Momand5Demilade Israel Ibiwoye6Department of Poultry Science, Faculty of Animal Husbandry and Veterinary Sciences, The University of AgricultureDepartment of Poultry Science, Faculty of Animal Husbandry and Veterinary Sciences, The University of AgricultureDepartment of Zoology, Government College UniversityDepartment of Zoology, College of Sciences, King Saud UniversityCollege of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of AgricultureCollege of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of AgriculturePrestage Department of Poultry Science, North Carolina State UniversityThis study aimed to evaluate the effects of enzyme treatment and fermentation of guar meal on growth performance, nutrient digestibility, and blood biochemistry in broiler chickens. A total of 900 day-old male Hubbard broiler chicks were divided into nine groups based on three levels of guar meal inclusion (3%, 6% and 9%) treated with enzymes (phytase, protease and β-mannanase) or fermented with Bacillus subtilis. The experiment followed a 3 × 3 factorial design, and growth parameters, nutrient digestibility, and blood biochemical analysis were conducted over a 21–day period. Significant (p < 0.05) improvements in feed intake and weight gain were observed in the enzyme-treated and fermented groups compared to untreated controls, particularly at 3% and 6% guar meal levels. Nutrient digestibility was higher for crude protein (p < 0.05) and calcium (p < 0.05) in enzyme-treated and fermented diets, with 3% and 6% inclusion levels showing better digestibility. Blood biochemical analysis indicated lower LDL (p < 0.05) in the fermented group and significant variations in triglycerides (p < 0.05) and HDL (p < 0.05) levels with different inclusion rates of guar meal. These findings suggest that enzyme treatment and fermentation of guar meal can enhance broiler growth performance, nutrient utilisation, and certain blood parameters, with the optimal effect seen at 3% and 6% inclusion levels.http://dx.doi.org/10.1080/1828051X.2025.2484442guar mealenzyme treatmentfermentationgrowth performancenutrient digestibility
spellingShingle Abdul Hafeez
Muhammad Haris
Shabana Naz
Abdulwahed Fahad Alrefaei
Rifat Ullah Khan
Naseer Khan Momand
Demilade Israel Ibiwoye
Impact of dietary enzyme and fermentation treatments on the performance, digestibility, and biochemical profile of broilers consuming guar meal
Italian Journal of Animal Science
guar meal
enzyme treatment
fermentation
growth performance
nutrient digestibility
title Impact of dietary enzyme and fermentation treatments on the performance, digestibility, and biochemical profile of broilers consuming guar meal
title_full Impact of dietary enzyme and fermentation treatments on the performance, digestibility, and biochemical profile of broilers consuming guar meal
title_fullStr Impact of dietary enzyme and fermentation treatments on the performance, digestibility, and biochemical profile of broilers consuming guar meal
title_full_unstemmed Impact of dietary enzyme and fermentation treatments on the performance, digestibility, and biochemical profile of broilers consuming guar meal
title_short Impact of dietary enzyme and fermentation treatments on the performance, digestibility, and biochemical profile of broilers consuming guar meal
title_sort impact of dietary enzyme and fermentation treatments on the performance digestibility and biochemical profile of broilers consuming guar meal
topic guar meal
enzyme treatment
fermentation
growth performance
nutrient digestibility
url http://dx.doi.org/10.1080/1828051X.2025.2484442
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