IgG detection in human serum employing non-functionalized chromium doped zinc gallate nanoparticles
Chromium-doped zinc gallate (ZnGa2O4:Cr3 +) nanoparticles (ZGO) show promising potential for antigen immunodetection using persistent luminescence, thereby reducing autofluorescence interference. Recently, we have shown that ZGO prepared by hydrothermal treatment at 120°C for 24 h can be used for in...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Next Nanotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949829525000683 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849420000054476800 |
|---|---|
| author | Zied Ferjaoui Capucine Zimmer Celina Matuszewska Corinne Chanéac Bruno Viana Cédric Bouzigues Daniel Scherman Nathalie Mignet Cyrille Richard |
| author_facet | Zied Ferjaoui Capucine Zimmer Celina Matuszewska Corinne Chanéac Bruno Viana Cédric Bouzigues Daniel Scherman Nathalie Mignet Cyrille Richard |
| author_sort | Zied Ferjaoui |
| collection | DOAJ |
| description | Chromium-doped zinc gallate (ZnGa2O4:Cr3 +) nanoparticles (ZGO) show promising potential for antigen immunodetection using persistent luminescence, thereby reducing autofluorescence interference. Recently, we have shown that ZGO prepared by hydrothermal treatment at 120°C for 24 h can be used for in vitro biodetection in simple media such as phosphate-buffered saline. In this study, we investigated the effect of the protocol used to synthesize these ZGO nanoparticles, using a hydrothermal treatment at 220°C for different durations (6 h, 12 h, and 24 h), followed by calcination at 500°C. The nanoparticle size determined by transmission electron microscopy after grinding and centrifugation was found to be around 15 nm. The persistent luminescence signal of the ZGO nanoparticles varied with the hydrothermal synthesis conditions. Moreover, in the presence of H2O2, these nanoparticles show a signal enhancement dependent on the hydrothermal duration, with a 12 h treatment showing the highest 8-fold luminescence increase in the presence of H2O2 produced by glucose oxidase mediated glucose degradation. Based on these results, these non-functionalized nanoparticles were successfully used to develop a persistent luminescence-based sandwich immunoassay for autofluorescence-free detection of antigens in undiluted human serum samples, using rabbit IgG as a model antigen. This study highlights the promising potential for biosensing applications of persistent ZGO nanophosphors for IgG detection in a complex medium (undiluted human serum), with a linear range from 1 ng mL−1 to 104 ng mL−1 and a limit of detection of 0.01 ng mL−1. The present optimization of ZGO nanophosphor synthesis offers promising prospects for medical diagnostics due to their increased sensitivity and ability to eliminate autofluorescence interference, as well as their ease of use, since no functionalization of the ZGO NPs is required before use. |
| format | Article |
| id | doaj-art-577a292fffc448bf89313a9aa1b33ab6 |
| institution | Kabale University |
| issn | 2949-8295 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Next Nanotechnology |
| spelling | doaj-art-577a292fffc448bf89313a9aa1b33ab62025-08-20T03:31:53ZengElsevierNext Nanotechnology2949-82952025-01-01810019910.1016/j.nxnano.2025.100199IgG detection in human serum employing non-functionalized chromium doped zinc gallate nanoparticlesZied Ferjaoui0Capucine Zimmer1Celina Matuszewska2Corinne Chanéac3Bruno Viana4Cédric Bouzigues5Daniel Scherman6Nathalie Mignet7Cyrille Richard8Université Paris Cité, CNRS, INSERM, UTCBS, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris 75006, FranceUniversité Paris Cité, CNRS, INSERM, UTCBS, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris 75006, FranceSorbonne Université, CNRS, Collège de France, Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), Paris 75005, FranceSorbonne Université, CNRS, Collège de France, Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), Paris 75005, FranceUniversité PSL, CNRS, IRCP, Chimie ParisTech, Paris, FranceLOB, Ecole Polytechnique, Palaiseau, FranceUniversité Paris Cité, CNRS, INSERM, UTCBS, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris 75006, FranceUniversité Paris Cité, CNRS, INSERM, UTCBS, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris 75006, FranceUniversité Paris Cité, CNRS, INSERM, UTCBS, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris 75006, France; Corresponding author.Chromium-doped zinc gallate (ZnGa2O4:Cr3 +) nanoparticles (ZGO) show promising potential for antigen immunodetection using persistent luminescence, thereby reducing autofluorescence interference. Recently, we have shown that ZGO prepared by hydrothermal treatment at 120°C for 24 h can be used for in vitro biodetection in simple media such as phosphate-buffered saline. In this study, we investigated the effect of the protocol used to synthesize these ZGO nanoparticles, using a hydrothermal treatment at 220°C for different durations (6 h, 12 h, and 24 h), followed by calcination at 500°C. The nanoparticle size determined by transmission electron microscopy after grinding and centrifugation was found to be around 15 nm. The persistent luminescence signal of the ZGO nanoparticles varied with the hydrothermal synthesis conditions. Moreover, in the presence of H2O2, these nanoparticles show a signal enhancement dependent on the hydrothermal duration, with a 12 h treatment showing the highest 8-fold luminescence increase in the presence of H2O2 produced by glucose oxidase mediated glucose degradation. Based on these results, these non-functionalized nanoparticles were successfully used to develop a persistent luminescence-based sandwich immunoassay for autofluorescence-free detection of antigens in undiluted human serum samples, using rabbit IgG as a model antigen. This study highlights the promising potential for biosensing applications of persistent ZGO nanophosphors for IgG detection in a complex medium (undiluted human serum), with a linear range from 1 ng mL−1 to 104 ng mL−1 and a limit of detection of 0.01 ng mL−1. The present optimization of ZGO nanophosphor synthesis offers promising prospects for medical diagnostics due to their increased sensitivity and ability to eliminate autofluorescence interference, as well as their ease of use, since no functionalization of the ZGO NPs is required before use.http://www.sciencedirect.com/science/article/pii/S2949829525000683Persistent luminescenceNon-functionalized nanoparticlesChromium-doped zinc gallateH2O2Signal enhancementBiosensing immunoassay |
| spellingShingle | Zied Ferjaoui Capucine Zimmer Celina Matuszewska Corinne Chanéac Bruno Viana Cédric Bouzigues Daniel Scherman Nathalie Mignet Cyrille Richard IgG detection in human serum employing non-functionalized chromium doped zinc gallate nanoparticles Next Nanotechnology Persistent luminescence Non-functionalized nanoparticles Chromium-doped zinc gallate H2O2 Signal enhancement Biosensing immunoassay |
| title | IgG detection in human serum employing non-functionalized chromium doped zinc gallate nanoparticles |
| title_full | IgG detection in human serum employing non-functionalized chromium doped zinc gallate nanoparticles |
| title_fullStr | IgG detection in human serum employing non-functionalized chromium doped zinc gallate nanoparticles |
| title_full_unstemmed | IgG detection in human serum employing non-functionalized chromium doped zinc gallate nanoparticles |
| title_short | IgG detection in human serum employing non-functionalized chromium doped zinc gallate nanoparticles |
| title_sort | igg detection in human serum employing non functionalized chromium doped zinc gallate nanoparticles |
| topic | Persistent luminescence Non-functionalized nanoparticles Chromium-doped zinc gallate H2O2 Signal enhancement Biosensing immunoassay |
| url | http://www.sciencedirect.com/science/article/pii/S2949829525000683 |
| work_keys_str_mv | AT ziedferjaoui iggdetectioninhumanserumemployingnonfunctionalizedchromiumdopedzincgallatenanoparticles AT capucinezimmer iggdetectioninhumanserumemployingnonfunctionalizedchromiumdopedzincgallatenanoparticles AT celinamatuszewska iggdetectioninhumanserumemployingnonfunctionalizedchromiumdopedzincgallatenanoparticles AT corinnechaneac iggdetectioninhumanserumemployingnonfunctionalizedchromiumdopedzincgallatenanoparticles AT brunoviana iggdetectioninhumanserumemployingnonfunctionalizedchromiumdopedzincgallatenanoparticles AT cedricbouzigues iggdetectioninhumanserumemployingnonfunctionalizedchromiumdopedzincgallatenanoparticles AT danielscherman iggdetectioninhumanserumemployingnonfunctionalizedchromiumdopedzincgallatenanoparticles AT nathaliemignet iggdetectioninhumanserumemployingnonfunctionalizedchromiumdopedzincgallatenanoparticles AT cyrillerichard iggdetectioninhumanserumemployingnonfunctionalizedchromiumdopedzincgallatenanoparticles |