Antibacterial Activity of Lactuca virosa with an In silico Approach
The current work aims to screen the phytoconstitutes of the Lactuca virosa leaves ethanolic extract by using GC-MS analysis and investigate its antibacterial activity. GC-MS analysis was conducted to identify the various phytochemical constituents within the ethanolic extracts of Lactuca vi...
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| Format: | Article |
| Language: | English |
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Krupanidhi College of Pharmacy
2024-06-01
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| Series: | Journal of Pharmaceutical Research |
| Online Access: | https://jopcr.com/articles/antibacterial-activity-of-lactuca-virosa-with-an-in-silico-approach |
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| author | Darshan Neelagund Rajesh Kumar Rawri C K Prasanna Paramita Das |
| author_facet | Darshan Neelagund Rajesh Kumar Rawri C K Prasanna Paramita Das |
| author_sort | Darshan Neelagund |
| collection | DOAJ |
| description | The current work aims to screen the phytoconstitutes of the Lactuca virosa leaves ethanolic extract by using GC-MS analysis and investigate its antibacterial activity. GC-MS analysis was conducted to identify the various phytochemical constituents within the ethanolic extracts of Lactuca virosa. Subsequently, protein-ligand docking was performed using proteins PDBID: 6AHT and 5C5H, revealing a strong affinity between the bioactive compounds and the proteins, indicating potent inhibitory action. Furthermore, each concentration of Lactuca virosa was assessed for antibacterial activity using Minimum Inhibitory Concentration (MIC) against bacterial strains including Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Streptococcus mutans. Preliminary phytochemical testing revealed the presence of alkaloids, coumarins, flavonoids, glycosides, phenols, terpenoids, oils, and resins. GC-MS showed the presence of many bioactive compounds in extract. Docking results highlighted that two compounds exhibited the most favourable binding energies of approximately -8.1 kcal/mol and -8.5 kcal/mol with 6AHT, and -8.8 kcal/mol with 5C5H. The ethanolic extract of 0.4mg concentration has shown good antibacterial activity against gram positive bacteria. The study identifies a new source of antibacterial compounds, which could lead to the development of new drugs, particularly effective against gram positive strains like Bacillus cereus and Streptococcus mutans. Keywords: Lactuca virosa, GC-MS, Docking, ADMET, Antibacterial Activity |
| format | Article |
| id | doaj-art-577164d2e95645a9b5f50d5534eecc8a |
| institution | Kabale University |
| issn | 0973-7200 2454-8405 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Krupanidhi College of Pharmacy |
| record_format | Article |
| series | Journal of Pharmaceutical Research |
| spelling | doaj-art-577164d2e95645a9b5f50d5534eecc8a2025-08-21T09:34:08ZengKrupanidhi College of PharmacyJournal of Pharmaceutical Research0973-72002454-84052024-06-0123211111810.18579/jopcr/v23.2.51Antibacterial Activity of Lactuca virosa with an In silico ApproachDarshan NeelagundRajesh Kumar RawriC K PrasannaParamita Das The current work aims to screen the phytoconstitutes of the Lactuca virosa leaves ethanolic extract by using GC-MS analysis and investigate its antibacterial activity. GC-MS analysis was conducted to identify the various phytochemical constituents within the ethanolic extracts of Lactuca virosa. Subsequently, protein-ligand docking was performed using proteins PDBID: 6AHT and 5C5H, revealing a strong affinity between the bioactive compounds and the proteins, indicating potent inhibitory action. Furthermore, each concentration of Lactuca virosa was assessed for antibacterial activity using Minimum Inhibitory Concentration (MIC) against bacterial strains including Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Streptococcus mutans. Preliminary phytochemical testing revealed the presence of alkaloids, coumarins, flavonoids, glycosides, phenols, terpenoids, oils, and resins. GC-MS showed the presence of many bioactive compounds in extract. Docking results highlighted that two compounds exhibited the most favourable binding energies of approximately -8.1 kcal/mol and -8.5 kcal/mol with 6AHT, and -8.8 kcal/mol with 5C5H. The ethanolic extract of 0.4mg concentration has shown good antibacterial activity against gram positive bacteria. The study identifies a new source of antibacterial compounds, which could lead to the development of new drugs, particularly effective against gram positive strains like Bacillus cereus and Streptococcus mutans. Keywords: Lactuca virosa, GC-MS, Docking, ADMET, Antibacterial Activityhttps://jopcr.com/articles/antibacterial-activity-of-lactuca-virosa-with-an-in-silico-approach |
| spellingShingle | Darshan Neelagund Rajesh Kumar Rawri C K Prasanna Paramita Das Antibacterial Activity of Lactuca virosa with an In silico Approach Journal of Pharmaceutical Research |
| title | Antibacterial Activity of Lactuca virosa with an In silico Approach |
| title_full | Antibacterial Activity of Lactuca virosa with an In silico Approach |
| title_fullStr | Antibacterial Activity of Lactuca virosa with an In silico Approach |
| title_full_unstemmed | Antibacterial Activity of Lactuca virosa with an In silico Approach |
| title_short | Antibacterial Activity of Lactuca virosa with an In silico Approach |
| title_sort | antibacterial activity of lactuca virosa with an in silico approach |
| url | https://jopcr.com/articles/antibacterial-activity-of-lactuca-virosa-with-an-in-silico-approach |
| work_keys_str_mv | AT darshanneelagund antibacterialactivityoflactucavirosawithaninsilicoapproach AT rajeshkumarrawri antibacterialactivityoflactucavirosawithaninsilicoapproach AT ckprasanna antibacterialactivityoflactucavirosawithaninsilicoapproach AT paramitadas antibacterialactivityoflactucavirosawithaninsilicoapproach |