Optimising RNA extraction for paddy bulk soil samples for metatranscriptome sequencing

Optimising RNA extraction for paddy bulk soil samples for metatranscriptome sequencing

Abstract Objective Extraction of high-quality RNA is crucial for understanding the molecular dynamics of microbiomes in the growth and development of paddy plants. However, paddy soil poses challenges due to contaminants such as humic substances and its clayish nature, which lead to RNA adsorption a...

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Main Authors: Sumitra Sivaprakasam, Tan Yee Fan, Yukgehnaish Kumarasan, Erneeza Mohd Hata, Ganesan Vadamalai, Bent Petersen, Thomas Sicheritz-Pontén, Sivachandran Parimannan, Heera Rajandas
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Research Notes
Subjects:
RNA extraction
Paddy
Bulk soil
Metatranscriptome sequencing
Online Access:https://doi.org/10.1186/s13104-025-07342-9
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