In vitro Evaluation of Antioxidant and Antimicrobial Activity of Aqueous Aerial Parts Extracts of Euphorbia helioscopia L.
Antitumor, antiviral, antimicrobial and antioxidant activities were reported for Euphorbia helioscopia usage in traditional medicine. Therefore, minimum inhibitory and bactericidal concentrations (MIC, MBC) of E. helioscopia aqueous leaf and stem extracts were evaluated by broth microdilution assay....
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An-Najah National University
2024-03-01
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| Series: | مجلة جامعة النجاح للأبحاث العلوم الطبيعية |
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| Online Access: | https://journals.najah.edu/media/journals/full_texts/8_Ym6uWod.pdf |
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| author | Ghadeer Omar Lubna Abdallah Ghaleb Adwan |
| author_facet | Ghadeer Omar Lubna Abdallah Ghaleb Adwan |
| author_sort | Ghadeer Omar |
| collection | DOAJ |
| description | Antitumor, antiviral, antimicrobial and antioxidant activities were reported for Euphorbia helioscopia usage in traditional medicine. Therefore, minimum inhibitory and bactericidal concentrations (MIC, MBC) of E. helioscopia aqueous leaf and stem extracts were evaluated by broth microdilution assay. As well as their antioxidant activity was carried out using DPPH assay. Obtained results showed that MIC values of leaf extract ranged from 0.78±0.0 mg/mL to 12.5±0.0 mg/mL and stem extract ranged from 3.125±0.0 mg/mL to 25±0.0 mg/mL. While, MBC of leaf and stem extracts had a range from 6.25±0.0 mg/mL to 50±0.0 mg/mL and 25±0.0 mg/mL to 50±0.0 mg/mL, respectively. The current study demonstrated that E. helioscopia leaf and stem extracts had antioxidant activity. One-way ANOVA test, the P values for the leaf and stem aqueous extracts were equal to 0.997 and 0.058, respectively. Consequently, both extracts showed no significant difference relative to the standard ascorbic acid (P value > 0.01). In addition, statistical analyses showed no significant differences between the tested concentrations of leaf extract and tested concentrations of ascorbic acid at P value > 0.01. The same thing was obtained for tested concentrations of stem aqueous extract, except at concentrations 7.81 and 3.91 µg/mL which showed DPPH inhibition percent 4.30±0.63 and 0.59±0.10, respectively. This indicates that E. helioscopia has potential to serve as a natural antioxidant, which makes it a promising candidate for therapeutic applications. Moreover, the positive antibacterial recorded data could potentially be used to develop potent drugs that improve the management and treatment of variety bacterial illnesses. |
| format | Article |
| id | doaj-art-576d80580bdb4c1fb786aec4154b4ed5 |
| institution | Kabale University |
| issn | 1727-2114 2311-8865 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | An-Najah National University |
| record_format | Article |
| series | مجلة جامعة النجاح للأبحاث العلوم الطبيعية |
| spelling | doaj-art-576d80580bdb4c1fb786aec4154b4ed52025-08-20T04:00:44ZengAn-Najah National Universityمجلة جامعة النجاح للأبحاث العلوم الطبيعية1727-21142311-88652024-03-0138212512910.35552/anujr.a.38.2.2210In vitro Evaluation of Antioxidant and Antimicrobial Activity of Aqueous Aerial Parts Extracts of Euphorbia helioscopia L.Ghadeer Omar0Lubna Abdallah1Ghaleb Adwan2Department of Biology and Biotechnology, Faculty of Sciences, An-Najah National University, Nablus, PalestineDepartment of Biology and Biotechnology, Faculty of Sciences, An-Najah National University, Nablus, PalestineDepartment of Biology and Biotechnology, Faculty of Sciences, An-Najah National University, Nablus, PalestineAntitumor, antiviral, antimicrobial and antioxidant activities were reported for Euphorbia helioscopia usage in traditional medicine. Therefore, minimum inhibitory and bactericidal concentrations (MIC, MBC) of E. helioscopia aqueous leaf and stem extracts were evaluated by broth microdilution assay. As well as their antioxidant activity was carried out using DPPH assay. Obtained results showed that MIC values of leaf extract ranged from 0.78±0.0 mg/mL to 12.5±0.0 mg/mL and stem extract ranged from 3.125±0.0 mg/mL to 25±0.0 mg/mL. While, MBC of leaf and stem extracts had a range from 6.25±0.0 mg/mL to 50±0.0 mg/mL and 25±0.0 mg/mL to 50±0.0 mg/mL, respectively. The current study demonstrated that E. helioscopia leaf and stem extracts had antioxidant activity. One-way ANOVA test, the P values for the leaf and stem aqueous extracts were equal to 0.997 and 0.058, respectively. Consequently, both extracts showed no significant difference relative to the standard ascorbic acid (P value > 0.01). In addition, statistical analyses showed no significant differences between the tested concentrations of leaf extract and tested concentrations of ascorbic acid at P value > 0.01. The same thing was obtained for tested concentrations of stem aqueous extract, except at concentrations 7.81 and 3.91 µg/mL which showed DPPH inhibition percent 4.30±0.63 and 0.59±0.10, respectively. This indicates that E. helioscopia has potential to serve as a natural antioxidant, which makes it a promising candidate for therapeutic applications. Moreover, the positive antibacterial recorded data could potentially be used to develop potent drugs that improve the management and treatment of variety bacterial illnesses.https://journals.najah.edu/media/journals/full_texts/8_Ym6uWod.pdfantibacterial activityantioxidanteuphorbia helioscopiaaqueous leaf extractmic.aqueous stem extractmbc |
| spellingShingle | Ghadeer Omar Lubna Abdallah Ghaleb Adwan In vitro Evaluation of Antioxidant and Antimicrobial Activity of Aqueous Aerial Parts Extracts of Euphorbia helioscopia L. مجلة جامعة النجاح للأبحاث العلوم الطبيعية antibacterial activity antioxidant euphorbia helioscopia aqueous leaf extract mic. aqueous stem extract mbc |
| title | In vitro Evaluation of Antioxidant and Antimicrobial Activity of Aqueous Aerial Parts Extracts of Euphorbia helioscopia L. |
| title_full | In vitro Evaluation of Antioxidant and Antimicrobial Activity of Aqueous Aerial Parts Extracts of Euphorbia helioscopia L. |
| title_fullStr | In vitro Evaluation of Antioxidant and Antimicrobial Activity of Aqueous Aerial Parts Extracts of Euphorbia helioscopia L. |
| title_full_unstemmed | In vitro Evaluation of Antioxidant and Antimicrobial Activity of Aqueous Aerial Parts Extracts of Euphorbia helioscopia L. |
| title_short | In vitro Evaluation of Antioxidant and Antimicrobial Activity of Aqueous Aerial Parts Extracts of Euphorbia helioscopia L. |
| title_sort | in vitro evaluation of antioxidant and antimicrobial activity of aqueous aerial parts extracts of euphorbia helioscopia l |
| topic | antibacterial activity antioxidant euphorbia helioscopia aqueous leaf extract mic. aqueous stem extract mbc |
| url | https://journals.najah.edu/media/journals/full_texts/8_Ym6uWod.pdf |
| work_keys_str_mv | AT ghadeeromar invitroevaluationofantioxidantandantimicrobialactivityofaqueousaerialpartsextractsofeuphorbiahelioscopial AT lubnaabdallah invitroevaluationofantioxidantandantimicrobialactivityofaqueousaerialpartsextractsofeuphorbiahelioscopial AT ghalebadwan invitroevaluationofantioxidantandantimicrobialactivityofaqueousaerialpartsextractsofeuphorbiahelioscopial |