Application of ATR-FTIR Spectroscopy for Analysis of Salt Stress in Brussels Sprouts

Salt stress is one of the environmental stresses that significantly reduces crop productivity and quality worldwide. Methods to overcome salt stress include developing salt-resistant crops by inserting various resistance genes or to diagnosing and responding to the effects of salt stress at an early...

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Main Authors: Su-Min Yun, Cheol-Soo Kim, Jeung-Joo Lee, Jung-Sung Chung
Format: Article
Language:English
Published: MDPI AG 2024-08-01
Series:Metabolites
Subjects:
Online Access:https://www.mdpi.com/2218-1989/14/9/470
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author Su-Min Yun
Cheol-Soo Kim
Jeung-Joo Lee
Jung-Sung Chung
author_facet Su-Min Yun
Cheol-Soo Kim
Jeung-Joo Lee
Jung-Sung Chung
author_sort Su-Min Yun
collection DOAJ
description Salt stress is one of the environmental stresses that significantly reduces crop productivity and quality worldwide. Methods to overcome salt stress include developing salt-resistant crops by inserting various resistance genes or to diagnosing and responding to the effects of salt stress at an early stage. In this study, we investigate the effects of salinity stress on growth, photosynthetic efficiency, and metabolic changes in Brussels sprouts (<i>Brassica oleracea</i> var. gemmifera). Fresh weight and leaf area decreased significantly with increasing NaCl concentration, indicating that salinity stress has a detrimental effect on plant growth. However, chlorophyll fluorescence parameters did not show significant changes, suggesting that photosynthetic efficiency was not significantly affected over 10 days. Fourier transform infrared (FTIR) spectroscopy revealed notable metabolic adjustments, especially in lipids, plastids, proteins, and carbohydrates, indicating biosynthesis of protective compounds such as anthocyanins and proline in response to salinity stress. Pearson correlation analysis confirmed a strong relationship between NaCl concentration and the observed physiological and metabolic changes. The findings highlight the potential of FTIR spectroscopy as a non-destructive tool for early detection of salinity stress and timely intervention to improve crop resilience and yield. This study highlights the widespread application of FTIR spectroscopy in agricultural research to manage abiotic stresses in crops.
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spelling doaj-art-576588090f39457383cdc12c8a29a93f2025-08-20T01:55:41ZengMDPI AGMetabolites2218-19892024-08-0114947010.3390/metabo14090470Application of ATR-FTIR Spectroscopy for Analysis of Salt Stress in Brussels SproutsSu-Min Yun0Cheol-Soo Kim1Jeung-Joo Lee2Jung-Sung Chung3Division of Applied Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDepartment of Applied Biology, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Plant Medicine, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDepartment of Agronomy, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaSalt stress is one of the environmental stresses that significantly reduces crop productivity and quality worldwide. Methods to overcome salt stress include developing salt-resistant crops by inserting various resistance genes or to diagnosing and responding to the effects of salt stress at an early stage. In this study, we investigate the effects of salinity stress on growth, photosynthetic efficiency, and metabolic changes in Brussels sprouts (<i>Brassica oleracea</i> var. gemmifera). Fresh weight and leaf area decreased significantly with increasing NaCl concentration, indicating that salinity stress has a detrimental effect on plant growth. However, chlorophyll fluorescence parameters did not show significant changes, suggesting that photosynthetic efficiency was not significantly affected over 10 days. Fourier transform infrared (FTIR) spectroscopy revealed notable metabolic adjustments, especially in lipids, plastids, proteins, and carbohydrates, indicating biosynthesis of protective compounds such as anthocyanins and proline in response to salinity stress. Pearson correlation analysis confirmed a strong relationship between NaCl concentration and the observed physiological and metabolic changes. The findings highlight the potential of FTIR spectroscopy as a non-destructive tool for early detection of salinity stress and timely intervention to improve crop resilience and yield. This study highlights the widespread application of FTIR spectroscopy in agricultural research to manage abiotic stresses in crops.https://www.mdpi.com/2218-1989/14/9/470FTIR spectroscopymetabolic changesphotosynthetic efficiencysalt stress
spellingShingle Su-Min Yun
Cheol-Soo Kim
Jeung-Joo Lee
Jung-Sung Chung
Application of ATR-FTIR Spectroscopy for Analysis of Salt Stress in Brussels Sprouts
Metabolites
FTIR spectroscopy
metabolic changes
photosynthetic efficiency
salt stress
title Application of ATR-FTIR Spectroscopy for Analysis of Salt Stress in Brussels Sprouts
title_full Application of ATR-FTIR Spectroscopy for Analysis of Salt Stress in Brussels Sprouts
title_fullStr Application of ATR-FTIR Spectroscopy for Analysis of Salt Stress in Brussels Sprouts
title_full_unstemmed Application of ATR-FTIR Spectroscopy for Analysis of Salt Stress in Brussels Sprouts
title_short Application of ATR-FTIR Spectroscopy for Analysis of Salt Stress in Brussels Sprouts
title_sort application of atr ftir spectroscopy for analysis of salt stress in brussels sprouts
topic FTIR spectroscopy
metabolic changes
photosynthetic efficiency
salt stress
url https://www.mdpi.com/2218-1989/14/9/470
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AT cheolsookim applicationofatrftirspectroscopyforanalysisofsaltstressinbrusselssprouts
AT jeungjoolee applicationofatrftirspectroscopyforanalysisofsaltstressinbrusselssprouts
AT jungsungchung applicationofatrftirspectroscopyforanalysisofsaltstressinbrusselssprouts