Frequency and Antibiotics Resistance of Extended-Spectrum Beta-Lactamase (ESBLs) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Patients in Gaza Strip, Palestine
Introduction: Extended-Spectrum β-Lactamases (ESBLs) hydrolyze broadspectrum cephalosporin, monobactam, and penicillin. This study investigated ESBL-producing Escherichia coli and Klebsiella pneumoniae bacteria in the Gaza strip and explored their susceptibility to various antimicrobials to provi...
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Pasteur Institute of Iran
2021-09-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
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| Online Access: | https://jommid.pasteur.ac.ir/article-1-362-en.html |
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| author | Ghassan Tayh Nahed Al Laham Imene Fhoula Nahed Abedelateef Mohamed El-Laham Abed Elkader Elottol Karim Ben Slama |
| author_facet | Ghassan Tayh Nahed Al Laham Imene Fhoula Nahed Abedelateef Mohamed El-Laham Abed Elkader Elottol Karim Ben Slama |
| author_sort | Ghassan Tayh |
| collection | DOAJ |
| description | Introduction: Extended-Spectrum β-Lactamases (ESBLs) hydrolyze broadspectrum
cephalosporin, monobactam, and penicillin. This study investigated
ESBL-producing Escherichia coli and Klebsiella pneumoniae bacteria in the
Gaza strip and explored their susceptibility to various antimicrobials to provide a
reference for physicians in managing the hospital infection. Methods: Ninetysix
isolates, comprising 69 E. coli and 27 K. pneumoniae were obtained from
urine, wound, blood, and ear discharge samples from April-June 2013 in Gaza
hospitals. The ESBL-producing isolates were screened using the double-disc
diffusion test. Antibiotics susceptibility test was determined by the disc diffusion
method on Mueller-Hinton agar, and PCR identified β-lactamases genes.
Results: Our results revealed high rates of ESBL-producing K. pneumoniae
(59.3%) and E. coli (39.1%) among isolates. About 65.1% of ESBL-producing
isolates were susceptible to imipenem while exhibited 100% resistance to
cefotaxime and ampicillin and 74.4% to sulfamethoxazole/trimethoprim. Except
for imipenem, higher antibiotic resistance rates were observed among ESBL
producers than non-ESBL producers. This study showed that the antimicrobial
resistance and ESBLs were higher in K. pneumoniae isolates than E. coli
isolates, and most K. pneumoniae isolates harbored simultaneously two or three
β-lactamases-encoding genes. Conclusion: High ESBL-producing rates among
K. pneumoniae and E. coli isolates and higher resistance rates to antibiotics
among ESBL compared to non-ESBL producing isolates necessitate
antimicrobial resistance surveillance and molecular characterization of ESBLsproducing
bacteria to achieve a specific treatment. |
| format | Article |
| id | doaj-art-574e2bb2f6ea49d98e7255aa0fa52bde |
| institution | OA Journals |
| issn | 2345-5349 2345-5330 |
| language | English |
| publishDate | 2021-09-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Journal of Medical Microbiology and Infectious Diseases |
| spelling | doaj-art-574e2bb2f6ea49d98e7255aa0fa52bde2025-08-20T02:11:22ZengPasteur Institute of IranJournal of Medical Microbiology and Infectious Diseases2345-53492345-53302021-09-019313314110.52547/JoMMID.9.3.133Frequency and Antibiotics Resistance of Extended-Spectrum Beta-Lactamase (ESBLs) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Patients in Gaza Strip, PalestineGhassan Tayh0https://orcid.org/0000-0002-7180-6787Nahed Al Laham1https://orcid.org/0000-0002-7019-7975Imene Fhoula2https://orcid.org/0000-0001-6416-685XNahed Abedelateef3https://orcid.org/0000-0003-4158-982XMohamed El-Laham4https://orcid.org/0000-0003-1494-7943Abed Elkader Elottol5https://orcid.org/0000-0001-5281-6405Karim Ben Slama6https://orcid.org/0000-0001-5745-3930Laboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisie; Service de Microbiologie et d’Immunologie, Ecole Nationale de Médecine Vétérinaire, Univ. Manouba, Sidi Thabet, TunisieDepartment of Laboratory Medicine, Faculty of Applied Medical Sciences, AlAzhar University-Gaza, PalestineLaboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, TunisieDepartment of Biological Science, Al-Aqsa University –Gaza, PalestineDepartment of Biological Science and Medical Technology, Israa University – Gaza, Palestine5Medical Science Department, University College of Science and Technology - Gaza, PalestineLaboratoire des Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisie; Institut Supérieur des Sciences Biologiques Appliquées de Tunis- Université de Tunis El Manar, 1006 Tunis, TunisieIntroduction: Extended-Spectrum β-Lactamases (ESBLs) hydrolyze broadspectrum cephalosporin, monobactam, and penicillin. This study investigated ESBL-producing Escherichia coli and Klebsiella pneumoniae bacteria in the Gaza strip and explored their susceptibility to various antimicrobials to provide a reference for physicians in managing the hospital infection. Methods: Ninetysix isolates, comprising 69 E. coli and 27 K. pneumoniae were obtained from urine, wound, blood, and ear discharge samples from April-June 2013 in Gaza hospitals. The ESBL-producing isolates were screened using the double-disc diffusion test. Antibiotics susceptibility test was determined by the disc diffusion method on Mueller-Hinton agar, and PCR identified β-lactamases genes. Results: Our results revealed high rates of ESBL-producing K. pneumoniae (59.3%) and E. coli (39.1%) among isolates. About 65.1% of ESBL-producing isolates were susceptible to imipenem while exhibited 100% resistance to cefotaxime and ampicillin and 74.4% to sulfamethoxazole/trimethoprim. Except for imipenem, higher antibiotic resistance rates were observed among ESBL producers than non-ESBL producers. This study showed that the antimicrobial resistance and ESBLs were higher in K. pneumoniae isolates than E. coli isolates, and most K. pneumoniae isolates harbored simultaneously two or three β-lactamases-encoding genes. Conclusion: High ESBL-producing rates among K. pneumoniae and E. coli isolates and higher resistance rates to antibiotics among ESBL compared to non-ESBL producing isolates necessitate antimicrobial resistance surveillance and molecular characterization of ESBLsproducing bacteria to achieve a specific treatment.https://jommid.pasteur.ac.ir/article-1-362-en.htmlbeta-lactamase genesesbl isolatesantibiotics resistancecarbapeneme. coli; k. pneumonia |
| spellingShingle | Ghassan Tayh Nahed Al Laham Imene Fhoula Nahed Abedelateef Mohamed El-Laham Abed Elkader Elottol Karim Ben Slama Frequency and Antibiotics Resistance of Extended-Spectrum Beta-Lactamase (ESBLs) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Patients in Gaza Strip, Palestine Journal of Medical Microbiology and Infectious Diseases beta-lactamase genes esbl isolates antibiotics resistance carbapenem e. coli; k. pneumonia |
| title | Frequency and Antibiotics Resistance of Extended-Spectrum Beta-Lactamase (ESBLs) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Patients in Gaza Strip, Palestine |
| title_full | Frequency and Antibiotics Resistance of Extended-Spectrum Beta-Lactamase (ESBLs) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Patients in Gaza Strip, Palestine |
| title_fullStr | Frequency and Antibiotics Resistance of Extended-Spectrum Beta-Lactamase (ESBLs) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Patients in Gaza Strip, Palestine |
| title_full_unstemmed | Frequency and Antibiotics Resistance of Extended-Spectrum Beta-Lactamase (ESBLs) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Patients in Gaza Strip, Palestine |
| title_short | Frequency and Antibiotics Resistance of Extended-Spectrum Beta-Lactamase (ESBLs) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Patients in Gaza Strip, Palestine |
| title_sort | frequency and antibiotics resistance of extended spectrum beta lactamase esbls producing escherichia coli and klebsiella pneumoniae isolated from patients in gaza strip palestine |
| topic | beta-lactamase genes esbl isolates antibiotics resistance carbapenem e. coli; k. pneumonia |
| url | https://jommid.pasteur.ac.ir/article-1-362-en.html |
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