Effects of typical plant growth regulator chlormequat chloride on alkaloidal compounds in Corydalis yanhusuo and molecular mechanisms
The effects of chlormequat chloride, a typical plant growth regulator, on the medicinal herb Corydalis yanhusuo were investigated. A standardized field experiment was conducted to investigate the molecular mechanisms and variations in active compounds resulting from chlormequat chloride treatment. S...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324016555 |
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| author | Zixuan Du Jiayin Cao Jie Meng Heng Zhou Qing Hu Ling Li Yun Liao Shui Miao Wenting Li Shen Ji Tongshuai Wang |
| author_facet | Zixuan Du Jiayin Cao Jie Meng Heng Zhou Qing Hu Ling Li Yun Liao Shui Miao Wenting Li Shen Ji Tongshuai Wang |
| author_sort | Zixuan Du |
| collection | DOAJ |
| description | The effects of chlormequat chloride, a typical plant growth regulator, on the medicinal herb Corydalis yanhusuo were investigated. A standardized field experiment was conducted to investigate the molecular mechanisms and variations in active compounds resulting from chlormequat chloride treatment. Samples of C. yanhusuo were collected under controlled conditions and at varying doses of chlormequat chloride. The differential compounds identified in both control and treated groups of C. yanhusuo were primarily alkaloids, as determined by non-targeted metabolomics analysis. The metabolite content was determined through the precise quantification of 12 enriched alkaloidal compounds across various categories of C. yanhusuo using targeted metabolomics. A comprehensive metabolomics evaluation method was developed that focuses on pharmacodynamically active compounds. Transcriptomic analysis also facilitates the identification of differential genes and enzymes associated with alkaloid production between the two groups. Chlormequat chloride significantly increased the yield of C. yanhusuo, but the content of the quantitatively abundant alkaloids decreased. It was suggested that the level of cytochrome P450 enzymes, primarily involved in the biosynthesis of benzylisoquinoline alkaloids, was inhibited by chlormequat chloride. In conclusion, this study revealed a dose-dependent effect of chlormequat chloride on C. yanhusuo and its associated molecular mechanisms as determined by omics analysis. |
| format | Article |
| id | doaj-art-5744a95754fb411084bd8b6134e1577a |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-5744a95754fb411084bd8b6134e1577a2025-02-12T05:29:52ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117579Effects of typical plant growth regulator chlormequat chloride on alkaloidal compounds in Corydalis yanhusuo and molecular mechanismsZixuan Du0Jiayin Cao1Jie Meng2Heng Zhou3Qing Hu4Ling Li5Yun Liao6Shui Miao7Wenting Li8Shen Ji9Tongshuai Wang10Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Changning District, Shanghai 200336, China; NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, 1500 Zhangheng Road, Pudong New Area, Shanghai 201203, China; Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Pudong New Area, Shanghai 201203, ChinaNMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, 1500 Zhangheng Road, Pudong New Area, Shanghai 201203, China; Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Pudong New Area, Shanghai 201203, ChinaTongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Changning District, Shanghai 200336, ChinaNMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, 1500 Zhangheng Road, Pudong New Area, Shanghai 201203, ChinaNMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, 1500 Zhangheng Road, Pudong New Area, Shanghai 201203, ChinaTongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Changning District, Shanghai 200336, ChinaTongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Changning District, Shanghai 200336, ChinaNMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, 1500 Zhangheng Road, Pudong New Area, Shanghai 201203, ChinaNMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, 1500 Zhangheng Road, Pudong New Area, Shanghai 201203, ChinaNMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, 1500 Zhangheng Road, Pudong New Area, Shanghai 201203, China; Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Pudong New Area, Shanghai 201203, China; Corresponding author at: NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, 1500 Zhangheng Road, Pudong New Area, Shanghai 201203, China.Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Changning District, Shanghai 200336, China; Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 720 Xianxia Road, Changning District, Shanghai 200336, China; Corresponding author at: Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Changning District, Shanghai 200336, China.The effects of chlormequat chloride, a typical plant growth regulator, on the medicinal herb Corydalis yanhusuo were investigated. A standardized field experiment was conducted to investigate the molecular mechanisms and variations in active compounds resulting from chlormequat chloride treatment. Samples of C. yanhusuo were collected under controlled conditions and at varying doses of chlormequat chloride. The differential compounds identified in both control and treated groups of C. yanhusuo were primarily alkaloids, as determined by non-targeted metabolomics analysis. The metabolite content was determined through the precise quantification of 12 enriched alkaloidal compounds across various categories of C. yanhusuo using targeted metabolomics. A comprehensive metabolomics evaluation method was developed that focuses on pharmacodynamically active compounds. Transcriptomic analysis also facilitates the identification of differential genes and enzymes associated with alkaloid production between the two groups. Chlormequat chloride significantly increased the yield of C. yanhusuo, but the content of the quantitatively abundant alkaloids decreased. It was suggested that the level of cytochrome P450 enzymes, primarily involved in the biosynthesis of benzylisoquinoline alkaloids, was inhibited by chlormequat chloride. In conclusion, this study revealed a dose-dependent effect of chlormequat chloride on C. yanhusuo and its associated molecular mechanisms as determined by omics analysis.http://www.sciencedirect.com/science/article/pii/S0147651324016555Plant Growth RegulatorsCorydalis yanhusuo W.T. WangChlormequat ChlorideMetabolomicsTranscriptomics |
| spellingShingle | Zixuan Du Jiayin Cao Jie Meng Heng Zhou Qing Hu Ling Li Yun Liao Shui Miao Wenting Li Shen Ji Tongshuai Wang Effects of typical plant growth regulator chlormequat chloride on alkaloidal compounds in Corydalis yanhusuo and molecular mechanisms Ecotoxicology and Environmental Safety Plant Growth Regulators Corydalis yanhusuo W.T. Wang Chlormequat Chloride Metabolomics Transcriptomics |
| title | Effects of typical plant growth regulator chlormequat chloride on alkaloidal compounds in Corydalis yanhusuo and molecular mechanisms |
| title_full | Effects of typical plant growth regulator chlormequat chloride on alkaloidal compounds in Corydalis yanhusuo and molecular mechanisms |
| title_fullStr | Effects of typical plant growth regulator chlormequat chloride on alkaloidal compounds in Corydalis yanhusuo and molecular mechanisms |
| title_full_unstemmed | Effects of typical plant growth regulator chlormequat chloride on alkaloidal compounds in Corydalis yanhusuo and molecular mechanisms |
| title_short | Effects of typical plant growth regulator chlormequat chloride on alkaloidal compounds in Corydalis yanhusuo and molecular mechanisms |
| title_sort | effects of typical plant growth regulator chlormequat chloride on alkaloidal compounds in corydalis yanhusuo and molecular mechanisms |
| topic | Plant Growth Regulators Corydalis yanhusuo W.T. Wang Chlormequat Chloride Metabolomics Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324016555 |
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