Industrial Scale Production and Characterization of a Whey Fraction Enriched in Extracellular Vesicle Material
ABSTRACT Human milk serves the sole nutritional role for the developing infant. During lactation, nano‐sized extracellular vesicles (EVs) in milk containing a multitude of biologically active components are transferred from mother to offspring. Infant formula (IF) based on cow milk‐derived ingredien...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2025-05-01
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| Series: | Journal of Extracellular Biology |
| Online Access: | https://doi.org/10.1002/jex2.70044 |
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| _version_ | 1850255625237299200 |
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| author | Søren Roi Midtgaard Maria Stenum Hansen Nikolaj Drachmann Xiaolu Geng Kristine Ingrid Marie Blans Manja Mahmens Fabricius Møbjerg Anny F. Frølund Jan Trige Rasmussen Marie Stampe Ostenfeld |
| author_facet | Søren Roi Midtgaard Maria Stenum Hansen Nikolaj Drachmann Xiaolu Geng Kristine Ingrid Marie Blans Manja Mahmens Fabricius Møbjerg Anny F. Frølund Jan Trige Rasmussen Marie Stampe Ostenfeld |
| author_sort | Søren Roi Midtgaard |
| collection | DOAJ |
| description | ABSTRACT Human milk serves the sole nutritional role for the developing infant. During lactation, nano‐sized extracellular vesicles (EVs) in milk containing a multitude of biologically active components are transferred from mother to offspring. Infant formula (IF) based on cow milk‐derived ingredients has been reported to contain reduced levels of EVs as compared to human milk. There is therefore an unmet need to produce large‐scale volumes of milk EVs to improve IF composition. Here, we report a scalable industrial production protocol for a bovine whey‐derived ingredient that is highly enriched in EV material using a large‐scale sequential ceramic membrane filtration setup. Furthermore, we demonstrate a robust and generally applicable analytical approach to determine the relative contributions of EVs and milk fat globule membrane (MFGM) using molar ratios of the membrane‐bound proteins butyrophilin (BTN) and CD9 as surrogate markers for MFGM and EVs, respectively. Taken together, our findings provide a basis for comparing bovine milk‐containing foods and aid in developing specialized ingredients that can minimize the compositional difference between infant formula and human milk. |
| format | Article |
| id | doaj-art-574375fe0ce54c2ba9a8f2b5d350282b |
| institution | OA Journals |
| issn | 2768-2811 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Biology |
| spelling | doaj-art-574375fe0ce54c2ba9a8f2b5d350282b2025-08-20T01:56:49ZengWileyJournal of Extracellular Biology2768-28112025-05-0145n/an/a10.1002/jex2.70044Industrial Scale Production and Characterization of a Whey Fraction Enriched in Extracellular Vesicle MaterialSøren Roi Midtgaard0Maria Stenum Hansen1Nikolaj Drachmann2Xiaolu Geng3Kristine Ingrid Marie Blans4Manja Mahmens Fabricius Møbjerg5Anny F. Frølund6Jan Trige Rasmussen7Marie Stampe Ostenfeld8Arla Foods Ingredients Group PS Viby Central Jutland Region DenmarkArla Foods Ingredients Group PS Viby Central Jutland Region DenmarkArla Foods Ingredients Group PS Viby Central Jutland Region DenmarkArla Foods Ingredients Group PS Viby Central Jutland Region DenmarkArla Foods Ingredients Group PS Viby Central Jutland Region DenmarkArla Foods Ingredients Group PS Viby Central Jutland Region DenmarkArla Foods Ingredients Group PS Viby Central Jutland Region DenmarkAarhus Universitet Aarhus Central Jutland Region DenmarkArla Foods Ingredients Group PS Viby Central Jutland Region DenmarkABSTRACT Human milk serves the sole nutritional role for the developing infant. During lactation, nano‐sized extracellular vesicles (EVs) in milk containing a multitude of biologically active components are transferred from mother to offspring. Infant formula (IF) based on cow milk‐derived ingredients has been reported to contain reduced levels of EVs as compared to human milk. There is therefore an unmet need to produce large‐scale volumes of milk EVs to improve IF composition. Here, we report a scalable industrial production protocol for a bovine whey‐derived ingredient that is highly enriched in EV material using a large‐scale sequential ceramic membrane filtration setup. Furthermore, we demonstrate a robust and generally applicable analytical approach to determine the relative contributions of EVs and milk fat globule membrane (MFGM) using molar ratios of the membrane‐bound proteins butyrophilin (BTN) and CD9 as surrogate markers for MFGM and EVs, respectively. Taken together, our findings provide a basis for comparing bovine milk‐containing foods and aid in developing specialized ingredients that can minimize the compositional difference between infant formula and human milk.https://doi.org/10.1002/jex2.70044 |
| spellingShingle | Søren Roi Midtgaard Maria Stenum Hansen Nikolaj Drachmann Xiaolu Geng Kristine Ingrid Marie Blans Manja Mahmens Fabricius Møbjerg Anny F. Frølund Jan Trige Rasmussen Marie Stampe Ostenfeld Industrial Scale Production and Characterization of a Whey Fraction Enriched in Extracellular Vesicle Material Journal of Extracellular Biology |
| title | Industrial Scale Production and Characterization of a Whey Fraction Enriched in Extracellular Vesicle Material |
| title_full | Industrial Scale Production and Characterization of a Whey Fraction Enriched in Extracellular Vesicle Material |
| title_fullStr | Industrial Scale Production and Characterization of a Whey Fraction Enriched in Extracellular Vesicle Material |
| title_full_unstemmed | Industrial Scale Production and Characterization of a Whey Fraction Enriched in Extracellular Vesicle Material |
| title_short | Industrial Scale Production and Characterization of a Whey Fraction Enriched in Extracellular Vesicle Material |
| title_sort | industrial scale production and characterization of a whey fraction enriched in extracellular vesicle material |
| url | https://doi.org/10.1002/jex2.70044 |
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