Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway
Abstract This study aimed to evaluate the therapeutic properties of the traditional Chinese medicine Xuesanqi (XSQ, from the rhizome of Polygonum amplexicaule D. Don) in treating ulcerative colitis. We hypothesized that its many active components can alleviate symptoms of colitis by regulating the g...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2024-12-01
|
| Series: | Natural Products and Bioprospecting |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s13659-024-00482-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850107492679286784 |
|---|---|
| author | Qiyuan Su Qian Hu Songtao Wu Suqin Yang Hanwen Su Zhengjun Zhang Chengxiu Ling |
| author_facet | Qiyuan Su Qian Hu Songtao Wu Suqin Yang Hanwen Su Zhengjun Zhang Chengxiu Ling |
| author_sort | Qiyuan Su |
| collection | DOAJ |
| description | Abstract This study aimed to evaluate the therapeutic properties of the traditional Chinese medicine Xuesanqi (XSQ, from the rhizome of Polygonum amplexicaule D. Don) in treating ulcerative colitis. We hypothesized that its many active components can alleviate symptoms of colitis by regulating the gut microbiota, its metabolites, and various signaling pathways. To test our hypotheses, we designed a DSS- induced colitis model in C57BL/6 male mice. Apparent metrics were evaluated in each group of mice and performed histological analysis of relevant tissues. The gut microbial composition was analyzed by 16S rRNA sequencing of bacteria. Simultaneously, the SCFAs content was detected by gas chromatography, inflammatory factor secretion was evaluated by ELISA or western-blot, the expression of tight junction protein and key proteins of the MAPK signaling pathway were analyzed by western-blot. Our result showed that the treatment with XSQ alleviated significant various symptoms such as weight loss, blood in stool, and shortening of colon. In addition, XSQ treatment restored the dysregulated gut microbiota in colitis mice, increased short chain fatty acids (SCFAs) and normalized the MAPK/ERK/JNK signaling pathways, promoted expression of tight junction protein Occludin, Claudin-1, and E-cadherin proteins. Furthermore, we also observed a dose-dependent pattern in these treatment responses. These findings demonstrated the active components of XSQ is a promising new treatment platform for ulcerative colitis. Graphical Abstract |
| format | Article |
| id | doaj-art-573ecd3313ac48e9b66baf1b91edb7f2 |
| institution | OA Journals |
| issn | 2192-2195 2192-2209 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Natural Products and Bioprospecting |
| spelling | doaj-art-573ecd3313ac48e9b66baf1b91edb7f22025-08-20T02:38:35ZengSpringerOpenNatural Products and Bioprospecting2192-21952192-22092024-12-0114111710.1007/s13659-024-00482-8Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathwayQiyuan Su0Qian Hu1Songtao Wu2Suqin Yang3Hanwen Su4Zhengjun Zhang5Chengxiu Ling6Wisdom Lake Academy of Pharmacy, Xi’an Jiaotong-Liverpool UniversityWuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFaculty of Pharmacy, Hubei University of Chinese MedicineSchool of Pharmaceutical Sciences, South-Central Minzu UniversityDepartment of Clinical Laboratory, Renmin Hospital of Wuhan UniversityDepartment of Statistics, University of Wisconsin-MadisonWisdom Lake Academy of Pharmacy, Xi’an Jiaotong-Liverpool UniversityAbstract This study aimed to evaluate the therapeutic properties of the traditional Chinese medicine Xuesanqi (XSQ, from the rhizome of Polygonum amplexicaule D. Don) in treating ulcerative colitis. We hypothesized that its many active components can alleviate symptoms of colitis by regulating the gut microbiota, its metabolites, and various signaling pathways. To test our hypotheses, we designed a DSS- induced colitis model in C57BL/6 male mice. Apparent metrics were evaluated in each group of mice and performed histological analysis of relevant tissues. The gut microbial composition was analyzed by 16S rRNA sequencing of bacteria. Simultaneously, the SCFAs content was detected by gas chromatography, inflammatory factor secretion was evaluated by ELISA or western-blot, the expression of tight junction protein and key proteins of the MAPK signaling pathway were analyzed by western-blot. Our result showed that the treatment with XSQ alleviated significant various symptoms such as weight loss, blood in stool, and shortening of colon. In addition, XSQ treatment restored the dysregulated gut microbiota in colitis mice, increased short chain fatty acids (SCFAs) and normalized the MAPK/ERK/JNK signaling pathways, promoted expression of tight junction protein Occludin, Claudin-1, and E-cadherin proteins. Furthermore, we also observed a dose-dependent pattern in these treatment responses. These findings demonstrated the active components of XSQ is a promising new treatment platform for ulcerative colitis. Graphical Abstracthttps://doi.org/10.1007/s13659-024-00482-8ColitisXuesanqiGut microbiotaShort chain fatty acids (SCFas)MAPK/ERK/JNK signaling pathwayTight junction (T.J.) protein |
| spellingShingle | Qiyuan Su Qian Hu Songtao Wu Suqin Yang Hanwen Su Zhengjun Zhang Chengxiu Ling Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway Natural Products and Bioprospecting Colitis Xuesanqi Gut microbiota Short chain fatty acids (SCFas) MAPK/ERK/JNK signaling pathway Tight junction (T.J.) protein |
| title | Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway |
| title_full | Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway |
| title_fullStr | Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway |
| title_full_unstemmed | Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway |
| title_short | Xuesanqi ameliorates DSS-induced colitis in mice by mediating gut microbiota dysbiosis and modulating MAPK/ERK/JNK pathway |
| title_sort | xuesanqi ameliorates dss induced colitis in mice by mediating gut microbiota dysbiosis and modulating mapk erk jnk pathway |
| topic | Colitis Xuesanqi Gut microbiota Short chain fatty acids (SCFas) MAPK/ERK/JNK signaling pathway Tight junction (T.J.) protein |
| url | https://doi.org/10.1007/s13659-024-00482-8 |
| work_keys_str_mv | AT qiyuansu xuesanqiamelioratesdssinducedcolitisinmicebymediatinggutmicrobiotadysbiosisandmodulatingmapkerkjnkpathway AT qianhu xuesanqiamelioratesdssinducedcolitisinmicebymediatinggutmicrobiotadysbiosisandmodulatingmapkerkjnkpathway AT songtaowu xuesanqiamelioratesdssinducedcolitisinmicebymediatinggutmicrobiotadysbiosisandmodulatingmapkerkjnkpathway AT suqinyang xuesanqiamelioratesdssinducedcolitisinmicebymediatinggutmicrobiotadysbiosisandmodulatingmapkerkjnkpathway AT hanwensu xuesanqiamelioratesdssinducedcolitisinmicebymediatinggutmicrobiotadysbiosisandmodulatingmapkerkjnkpathway AT zhengjunzhang xuesanqiamelioratesdssinducedcolitisinmicebymediatinggutmicrobiotadysbiosisandmodulatingmapkerkjnkpathway AT chengxiuling xuesanqiamelioratesdssinducedcolitisinmicebymediatinggutmicrobiotadysbiosisandmodulatingmapkerkjnkpathway |