Antibacterial Action, Antioxidant Activity and Anticoagulant Effect of Pectin Extracted from Peels of Algerian Citrus Sinensis
In this study, we characterised the pectin extracted from peels of Algerian Citrus sinensis and evaluated its antibacterial action, antioxidant activity and anticoagulant effect. Pectin was extracted under acidic conditions using hydrochloric acid for PCT-1 and citric acid for PCT-2 and determining...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
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| Series: | Chemistry Proceedings |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2673-4583/14/1/69 |
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| Summary: | In this study, we characterised the pectin extracted from peels of Algerian Citrus sinensis and evaluated its antibacterial action, antioxidant activity and anticoagulant effect. Pectin was extracted under acidic conditions using hydrochloric acid for PCT-1 and citric acid for PCT-2 and determining their physicochemical properties using Fourier-Transform Infrared spectroscopy (FTIR), X-ray powder diffraction (PXRD), differential scanning calorimetry (DSC), yield, the degree of methylation, water content and ash content. In addition, the FTIR results showed desired banding characteristics, and their thermal properties evaluated using DSC showed that the thermal degradation was around 240 °C. XRD results show that PCT-1 and PCT-2 are amorphous and have similar characteristics to commercial pectin. On the other hand, the antibacterial action showed that PCT-1 and PCT-2 have no effect on <i>Pseudomonas aeruginosa</i> and <i>E. coli</i> bacteria, unlike <i>Staphylococcus epidermidis</i>, where it showed considerable antibacterial action. The antioxidant activity of PCT-1 and PCT-2 was observed using the 2,2 diphenyl-1-picrylhydrazyl (DPPH) method; the absorbance values recorded for PCT-1 and PCT-2 confirmed their antioxidant potential explained by the presence of several free hydroxyl groups in the PCT-1 and PCT-2 structure. On the other hand, our findings indicate that PCT-1 and PCT-2 do not have a marked anticoagulant effect but have acceptable potential and can be used as anticoagulants for the treatment of thrombotic diseases with fewer side effects compared to the widely used heparin. These results suggest that pectin from the peels of Algerian Citrus sinensis has potential properties as a biomaterial for several biomedical applications. |
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| ISSN: | 2673-4583 |