Insights into Orris (<i>Iris pallida</i> Lam.) In Vivo Acclimatization and Response to Salt Stress via Exogenous Melatonin Application
The loss of agricultural land is one of the main problems facing the global agricultural sector, and it is related to multiple phenomena; one of the main causes is soil salinization, induced both by natural processes and human activities. Among the strategies adopted to deal with soil salinization a...
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MDPI AG
2024-12-01
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| author | Annalisa Meucci Cristina Ghelardi Rita Maggini Fernando Malorgio Beatrice Pezzarossa Irene Rosellini Anna Mensuali |
| author_facet | Annalisa Meucci Cristina Ghelardi Rita Maggini Fernando Malorgio Beatrice Pezzarossa Irene Rosellini Anna Mensuali |
| author_sort | Annalisa Meucci |
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| description | The loss of agricultural land is one of the main problems facing the global agricultural sector, and it is related to multiple phenomena; one of the main causes is soil salinization, induced both by natural processes and human activities. Among the strategies adopted to deal with soil salinization and its mitigation, the cultivation of species able to survive in saline soils seems to be an effective way of making salt-compromised lands usable. <i>Iris pallida</i> is a rustic plant and a species of high economic value that is mostly cultivated for perfume production. Consequently, the application of <i>I. pallida</i> to cover soils not suitable for crops traditionally cultivated for human and livestock nutrition could be considered; therefore, a preliminary test on the capacity of <i>I. pallida</i> to tolerate salinity during the acclimatization phase of micropropagated plants was conducted. Plantlets were treated with exogenous melatonin during the in vitro phase by adding it to the culture medium; therefore, during the acclimatization phase, crescent salt doses (150, 300, and 400 mM) were added to the soil every 14 days, administering melatonin to plants by a spray solution 24 h before each salt addition. At the end of the experiment, biometric measurements, chlorophylls, carotenoids, and macro-element contents were measured, and the relative water content (RWC) was determined in each salt addition. The results showed that orris plants can survive soil salt concentrations of up to 400 mM, and that the 50 µM melatonin spray treatment can protect orris rhizomes from salt side effects. |
| format | Article |
| id | doaj-art-573ce6470edf4f8080d72a7cad2ba968 |
| institution | DOAJ |
| issn | 2077-0472 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Agriculture |
| spelling | doaj-art-573ce6470edf4f8080d72a7cad2ba9682025-08-20T02:53:39ZengMDPI AGAgriculture2077-04722024-12-011412235310.3390/agriculture14122353Insights into Orris (<i>Iris pallida</i> Lam.) In Vivo Acclimatization and Response to Salt Stress via Exogenous Melatonin ApplicationAnnalisa Meucci0Cristina Ghelardi1Rita Maggini2Fernando Malorgio3Beatrice Pezzarossa4Irene Rosellini5Anna Mensuali6Institute of Crop Science, Sant’Anna School of Advanced Studies, Piazza Martiri della Libertà, 33, 56127 Pisa, ItalyInstitute of Crop Science, Sant’Anna School of Advanced Studies, Piazza Martiri della Libertà, 33, 56127 Pisa, ItalyDepartment of Agricultural, Food and Agro-Environmental Sciences, University of Pisa, Via del Borghetto, 80, 56124 Pisa, ItalyDepartment of Agricultural, Food and Agro-Environmental Sciences, University of Pisa, Via del Borghetto, 80, 56124 Pisa, ItalyInstitute of Research on Terrestrial Ecosystems (IRET), National Research Council (CNR), Via Moruzzi 1, 56124 Pisa, ItalyInstitute of Research on Terrestrial Ecosystems (IRET), National Research Council (CNR), Via Moruzzi 1, 56124 Pisa, ItalyInstitute of Crop Science, Sant’Anna School of Advanced Studies, Piazza Martiri della Libertà, 33, 56127 Pisa, ItalyThe loss of agricultural land is one of the main problems facing the global agricultural sector, and it is related to multiple phenomena; one of the main causes is soil salinization, induced both by natural processes and human activities. Among the strategies adopted to deal with soil salinization and its mitigation, the cultivation of species able to survive in saline soils seems to be an effective way of making salt-compromised lands usable. <i>Iris pallida</i> is a rustic plant and a species of high economic value that is mostly cultivated for perfume production. Consequently, the application of <i>I. pallida</i> to cover soils not suitable for crops traditionally cultivated for human and livestock nutrition could be considered; therefore, a preliminary test on the capacity of <i>I. pallida</i> to tolerate salinity during the acclimatization phase of micropropagated plants was conducted. Plantlets were treated with exogenous melatonin during the in vitro phase by adding it to the culture medium; therefore, during the acclimatization phase, crescent salt doses (150, 300, and 400 mM) were added to the soil every 14 days, administering melatonin to plants by a spray solution 24 h before each salt addition. At the end of the experiment, biometric measurements, chlorophylls, carotenoids, and macro-element contents were measured, and the relative water content (RWC) was determined in each salt addition. The results showed that orris plants can survive soil salt concentrations of up to 400 mM, and that the 50 µM melatonin spray treatment can protect orris rhizomes from salt side effects.https://www.mdpi.com/2077-0472/14/12/2353in vitrogrowth regulatorssalinityacclimatizationtolerance |
| spellingShingle | Annalisa Meucci Cristina Ghelardi Rita Maggini Fernando Malorgio Beatrice Pezzarossa Irene Rosellini Anna Mensuali Insights into Orris (<i>Iris pallida</i> Lam.) In Vivo Acclimatization and Response to Salt Stress via Exogenous Melatonin Application Agriculture in vitro growth regulators salinity acclimatization tolerance |
| title | Insights into Orris (<i>Iris pallida</i> Lam.) In Vivo Acclimatization and Response to Salt Stress via Exogenous Melatonin Application |
| title_full | Insights into Orris (<i>Iris pallida</i> Lam.) In Vivo Acclimatization and Response to Salt Stress via Exogenous Melatonin Application |
| title_fullStr | Insights into Orris (<i>Iris pallida</i> Lam.) In Vivo Acclimatization and Response to Salt Stress via Exogenous Melatonin Application |
| title_full_unstemmed | Insights into Orris (<i>Iris pallida</i> Lam.) In Vivo Acclimatization and Response to Salt Stress via Exogenous Melatonin Application |
| title_short | Insights into Orris (<i>Iris pallida</i> Lam.) In Vivo Acclimatization and Response to Salt Stress via Exogenous Melatonin Application |
| title_sort | insights into orris i iris pallida i lam in vivo acclimatization and response to salt stress via exogenous melatonin application |
| topic | in vitro growth regulators salinity acclimatization tolerance |
| url | https://www.mdpi.com/2077-0472/14/12/2353 |
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