PET Imaging of Hepatocellular Carcinomas: F-Fluoropropionic Acid as a Complementary Radiotracer for F-Fluorodeoxyglucose
Objective: To evaluate the preclinical value of 18 F-fluoropropionic acid ( 18 F-FPA) and 18 F-fluorodeoxyglucose ( 18 F-FDG) positron emission tomography (PET) for imaging HCCs. Methods: The 18 F-FPA and 18 F-FDG uptake patterns in 3 HCC cell lines (Hep3B, HepG2, and SK-Hep1) were assessed in vitro...
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| Language: | English |
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SAGE Publishing
2019-01-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1177/1536012118821032 |
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| author | Jing Zhao MMed Zhanwen Zhang MD Dahong Nie MD Hui Ma MD Gongjun Yuan MD Shu Su MMed Shaoyu Liu DSc Sheng Liu MD Ganghua Tang DSc |
| author_facet | Jing Zhao MMed Zhanwen Zhang MD Dahong Nie MD Hui Ma MD Gongjun Yuan MD Shu Su MMed Shaoyu Liu DSc Sheng Liu MD Ganghua Tang DSc |
| author_sort | Jing Zhao MMed |
| collection | DOAJ |
| description | Objective: To evaluate the preclinical value of 18 F-fluoropropionic acid ( 18 F-FPA) and 18 F-fluorodeoxyglucose ( 18 F-FDG) positron emission tomography (PET) for imaging HCCs. Methods: The 18 F-FPA and 18 F-FDG uptake patterns in 3 HCC cell lines (Hep3B, HepG2, and SK-Hep1) were assessed in vitro and in vivo. The 18 F-FPA uptake mechanism was investigated using inhibition experiments with orlistat and 5-tetradecyloxy-2-furoic acid. The 18 F-FPA PET imaging was performed in different tumor animal models and compared with 18 F-FDG. We also evaluated the expressions of glucose transporter-1 (GLUT1), fatty acid synthase (FASN), and matrix metalloproteinase-2 (MMP2) in these cell lines. Results: In vitro experiments showed that the radiotracer uptake patterns were complementary in the HCC cell lines. Orlistat and 5-tetradecyloxy-2-furoic acid decreased the uptake of 18 F-FPA. The tumor-to-liver ratio of 18 F-FPA was superior to that of 18 F-FDG in the SK-Hep1 and HepG2 tumors ( P < .05). However, in the Hep3B tumors, the tumor-to-liver normalized uptake of 18 F-FDG was higher than 18 F-FPA ( P < .01). FASN was highly expressed in cell lines with high 18 F-FPA uptake, whereas GLUT1 was highly expressed in cell lines with high 18 F-FDG uptake. The 18 F-FPA uptake correlated with FASN ( r = 0.89, P = .014) and MMP2 ( r = 0.77, P = .002) expressions. Conclusions: PET imaging with 18 F-FPA combined with 18 F-FDG can be an alternative for detecting HCC. |
| format | Article |
| id | doaj-art-573a4e66ba5c44e988a5f51ea60c99bd |
| institution | DOAJ |
| issn | 1536-0121 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-573a4e66ba5c44e988a5f51ea60c99bd2025-08-20T02:42:49ZengSAGE PublishingMolecular Imaging1536-01212019-01-011810.1177/1536012118821032PET Imaging of Hepatocellular Carcinomas: F-Fluoropropionic Acid as a Complementary Radiotracer for F-FluorodeoxyglucoseJing Zhao MMed0Zhanwen Zhang MD1Dahong Nie MD2Hui Ma MD3Gongjun Yuan MD4Shu Su MMed5Shaoyu Liu DSc6Sheng Liu MD7Ganghua Tang DSc8 Guangdong Engineering Research Center for Translational Application of Medical Radiopharmaceuticals, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Department of Nuclear Medicine, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Department of Radiation Oncology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Department of Nuclear Medicine and Imaging Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Department of Nuclear Medicine and Imaging Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Department of Nuclear Medicine and Imaging Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Department of Nuclear Medicine and Imaging Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Department of Nuclear Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China Department of Nuclear Medicine and Imaging Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaObjective: To evaluate the preclinical value of 18 F-fluoropropionic acid ( 18 F-FPA) and 18 F-fluorodeoxyglucose ( 18 F-FDG) positron emission tomography (PET) for imaging HCCs. Methods: The 18 F-FPA and 18 F-FDG uptake patterns in 3 HCC cell lines (Hep3B, HepG2, and SK-Hep1) were assessed in vitro and in vivo. The 18 F-FPA uptake mechanism was investigated using inhibition experiments with orlistat and 5-tetradecyloxy-2-furoic acid. The 18 F-FPA PET imaging was performed in different tumor animal models and compared with 18 F-FDG. We also evaluated the expressions of glucose transporter-1 (GLUT1), fatty acid synthase (FASN), and matrix metalloproteinase-2 (MMP2) in these cell lines. Results: In vitro experiments showed that the radiotracer uptake patterns were complementary in the HCC cell lines. Orlistat and 5-tetradecyloxy-2-furoic acid decreased the uptake of 18 F-FPA. The tumor-to-liver ratio of 18 F-FPA was superior to that of 18 F-FDG in the SK-Hep1 and HepG2 tumors ( P < .05). However, in the Hep3B tumors, the tumor-to-liver normalized uptake of 18 F-FDG was higher than 18 F-FPA ( P < .01). FASN was highly expressed in cell lines with high 18 F-FPA uptake, whereas GLUT1 was highly expressed in cell lines with high 18 F-FDG uptake. The 18 F-FPA uptake correlated with FASN ( r = 0.89, P = .014) and MMP2 ( r = 0.77, P = .002) expressions. Conclusions: PET imaging with 18 F-FPA combined with 18 F-FDG can be an alternative for detecting HCC.https://doi.org/10.1177/1536012118821032 |
| spellingShingle | Jing Zhao MMed Zhanwen Zhang MD Dahong Nie MD Hui Ma MD Gongjun Yuan MD Shu Su MMed Shaoyu Liu DSc Sheng Liu MD Ganghua Tang DSc PET Imaging of Hepatocellular Carcinomas: F-Fluoropropionic Acid as a Complementary Radiotracer for F-Fluorodeoxyglucose Molecular Imaging |
| title | PET Imaging of Hepatocellular Carcinomas: F-Fluoropropionic Acid as a Complementary Radiotracer for F-Fluorodeoxyglucose |
| title_full | PET Imaging of Hepatocellular Carcinomas: F-Fluoropropionic Acid as a Complementary Radiotracer for F-Fluorodeoxyglucose |
| title_fullStr | PET Imaging of Hepatocellular Carcinomas: F-Fluoropropionic Acid as a Complementary Radiotracer for F-Fluorodeoxyglucose |
| title_full_unstemmed | PET Imaging of Hepatocellular Carcinomas: F-Fluoropropionic Acid as a Complementary Radiotracer for F-Fluorodeoxyglucose |
| title_short | PET Imaging of Hepatocellular Carcinomas: F-Fluoropropionic Acid as a Complementary Radiotracer for F-Fluorodeoxyglucose |
| title_sort | pet imaging of hepatocellular carcinomas f fluoropropionic acid as a complementary radiotracer for f fluorodeoxyglucose |
| url | https://doi.org/10.1177/1536012118821032 |
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