Pathogenicity of Klebsiella pneumonia (KpC4) infecting maize and mice

Recently, a new bacterial top rot disease of maize has frequently appeared in many areas of Yunnan Province, China. The pathogen of the disease was identified as Klebsiella pneumoniae (KpC4), which is well known to cause pulmonary and urinary diseases in humans and animals and occasionally exists as...

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Main Authors: Min HUANG, Li LIN, Yi-xin WU, Ho Honhing, Peng-fei HE, Guo-zhi LI, Peng-bo HE, Guo-ru XIONG, Yuan YUAN, Yue-qiu HE
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2016-07-01
Series:Journal of Integrative Agriculture
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Online Access:http://www.sciencedirect.com/science/article/pii/S2095311916613345
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author Min HUANG
Li LIN
Yi-xin WU
Ho Honhing
Peng-fei HE
Guo-zhi LI
Peng-bo HE
Guo-ru XIONG
Yuan YUAN
Yue-qiu HE
author_facet Min HUANG
Li LIN
Yi-xin WU
Ho Honhing
Peng-fei HE
Guo-zhi LI
Peng-bo HE
Guo-ru XIONG
Yuan YUAN
Yue-qiu HE
author_sort Min HUANG
collection DOAJ
description Recently, a new bacterial top rot disease of maize has frequently appeared in many areas of Yunnan Province, China. The pathogen of the disease was identified as Klebsiella pneumoniae (KpC4), which is well known to cause pulmonary and urinary diseases in humans and animals and occasionally exists as a harmless endophyte in plants. To evaluate the virulence of the maize pathogen to maize and mice, we inoculated maize and mice with routine inoculation and intraperitoneal injection respectively according to Koch's postulates. The results showed that KpC4 and the clinical strain K. pneumoniae 138 (Kp138) were all highly pathogenic to maize and mice and the strain re-isolated from diseased mice also caused typical top rot symptoms on maize by artificial inoculation. It is highlighting that a seemingly dedicated human/animal pathogen could cause plant disease. This is the first report of K. pneumoniae, an opportunistic pathogen of human/animal, could infect maize and mice. The findings serve as an alert to plant, medical and veterinarian scientists regarding a potentially dangerous bacterial pathogen infecting both plants and animals/humans. The maize plants in the field could serve as a reservoir for K. pneumoniae which might infect animals and probably humans when conditions are favorable. The new findings not only are significant in the developing control strategy for the new disease in Yunnan, but also serve as a starting point for further studies on the mechanism of pathogenesis and epidemiology of K. pneumoniae.
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spelling doaj-art-573700fd8d4d49878cbc2cdaf3b75a122025-08-20T03:56:12ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192016-07-011571510152010.1016/S2095-3119(16)61334-5Pathogenicity of Klebsiella pneumonia (KpC4) infecting maize and miceMin HUANG0Li LIN1Yi-xin WU2Ho Honhing3Peng-fei HE4Guo-zhi LI5Peng-bo HE6Guo-ru XIONG7Yuan YUAN8Yue-qiu HE9Faculty of Plant Protection, Yunnan Agricultural University, Kunming 650201, P.R. China; Agriculture College and Urban Modern Agriculture Engineering Research Center, Kunming University, Kunming 650214, P.R. China; HUANG Min, Tel: +86-871-65228221Agriculture College and Urban Modern Agriculture Engineering Research Center, Kunming University, Kunming 650214, P.R. ChinaCollege of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, P.R. China; National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650236, P.R. ChinaDepartment of Biology, State University of New York, New Paltz 12561, USAFaculty of Plant Protection, Yunnan Agricultural University, Kunming 650201, P.R. ChinaCollege of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, P.R. ChinaFaculty of Plant Protection, Yunnan Agricultural University, Kunming 650201, P.R. ChinaInstitute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou 570102, P.R. ChinaFaculty of Plant Protection, Yunnan Agricultural University, Kunming 650201, P.R. ChinaFaculty of Plant Protection, Yunnan Agricultural University, Kunming 650201, P.R. China; National and Local Joint Engineering Research Center for Screening and Application of Microbial Strains, Kunming 650236, P.R. China; Correspondence HE Yue-qiuRecently, a new bacterial top rot disease of maize has frequently appeared in many areas of Yunnan Province, China. The pathogen of the disease was identified as Klebsiella pneumoniae (KpC4), which is well known to cause pulmonary and urinary diseases in humans and animals and occasionally exists as a harmless endophyte in plants. To evaluate the virulence of the maize pathogen to maize and mice, we inoculated maize and mice with routine inoculation and intraperitoneal injection respectively according to Koch's postulates. The results showed that KpC4 and the clinical strain K. pneumoniae 138 (Kp138) were all highly pathogenic to maize and mice and the strain re-isolated from diseased mice also caused typical top rot symptoms on maize by artificial inoculation. It is highlighting that a seemingly dedicated human/animal pathogen could cause plant disease. This is the first report of K. pneumoniae, an opportunistic pathogen of human/animal, could infect maize and mice. The findings serve as an alert to plant, medical and veterinarian scientists regarding a potentially dangerous bacterial pathogen infecting both plants and animals/humans. The maize plants in the field could serve as a reservoir for K. pneumoniae which might infect animals and probably humans when conditions are favorable. The new findings not only are significant in the developing control strategy for the new disease in Yunnan, but also serve as a starting point for further studies on the mechanism of pathogenesis and epidemiology of K. pneumoniae.http://www.sciencedirect.com/science/article/pii/S2095311916613345Klebsiella pneumoniaemaize top rot diseaseisolation and identificationpathogenicity testswhite mouse
spellingShingle Min HUANG
Li LIN
Yi-xin WU
Ho Honhing
Peng-fei HE
Guo-zhi LI
Peng-bo HE
Guo-ru XIONG
Yuan YUAN
Yue-qiu HE
Pathogenicity of Klebsiella pneumonia (KpC4) infecting maize and mice
Journal of Integrative Agriculture
Klebsiella pneumoniae
maize top rot disease
isolation and identification
pathogenicity tests
white mouse
title Pathogenicity of Klebsiella pneumonia (KpC4) infecting maize and mice
title_full Pathogenicity of Klebsiella pneumonia (KpC4) infecting maize and mice
title_fullStr Pathogenicity of Klebsiella pneumonia (KpC4) infecting maize and mice
title_full_unstemmed Pathogenicity of Klebsiella pneumonia (KpC4) infecting maize and mice
title_short Pathogenicity of Klebsiella pneumonia (KpC4) infecting maize and mice
title_sort pathogenicity of klebsiella pneumonia kpc4 infecting maize and mice
topic Klebsiella pneumoniae
maize top rot disease
isolation and identification
pathogenicity tests
white mouse
url http://www.sciencedirect.com/science/article/pii/S2095311916613345
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