Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34+ Cells
Inflammation may play a role in cancer. However, the contribution of cytokine-mediated crosstalk between normal hemopoietic stem/progenitor cells (HSPCs) and their (inflammatory) microenvironment is largely elusive. Here we compared survival, phenotype, and function of neonatal (umbilical cord blood...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/5974613 |
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| author | Dorian Forte Daria Sollazzo Martina Barone Marisole Allegri Angela di Martella Orsi Marco Romano Barbara Sinigaglia Giuseppe Auteri Nicola Vianelli Michele Cavo Francesca Palandri Lucia Catani |
| author_facet | Dorian Forte Daria Sollazzo Martina Barone Marisole Allegri Angela di Martella Orsi Marco Romano Barbara Sinigaglia Giuseppe Auteri Nicola Vianelli Michele Cavo Francesca Palandri Lucia Catani |
| author_sort | Dorian Forte |
| collection | DOAJ |
| description | Inflammation may play a role in cancer. However, the contribution of cytokine-mediated crosstalk between normal hemopoietic stem/progenitor cells (HSPCs) and their (inflammatory) microenvironment is largely elusive. Here we compared survival, phenotype, and function of neonatal (umbilical cord blood (CB)) and adult (normal G-CSF-mobilized peripheral blood (mPB)) CD34+ cells after in vitro exposure to combined crucial inflammatory factors such as interleukin- (IL-) 1β, IL-6, tumor necrosis factor- (TNF-) α, or tissue inhibitor of metalloproteinases-1 (TIMP-1). To mimic bone marrow (BM) niche, coculture experiments with normal BM stromal cells (BMSCs) were also performed. We found that combined inflammatory cytokines increased only the in vitro survival of CB-derived CD34+ cells by reducing apoptosis. Conversely, selected combinations of inflammatory cytokines (IL-1β + TNF-α, IL-6 + TNF-α, and IL-1β + TNF-α + TIMP-1) mainly enhanced the in vitro CXCR4-driven migration of mPB-derived CD34+ cells. TNF-α, alone or in combination, upregulated CD44 and CD13 expression in both sources. Finally, BMSCs alone increased survival/migration of CB- and mPB-derived CD34+ cells at the same extent of the combined inflammatory cytokines; importantly, their copresence did not show additive/synergistic effect. Taken together, these data indicate that combined proinflammatory stimuli promote distinct in vitro functional activation of neonatal or adult normal HSPCs. |
| format | Article |
| id | doaj-art-572a768e4dbe4e188a3de7cac45d685a |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-572a768e4dbe4e188a3de7cac45d685a2025-08-20T02:05:57ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/59746135974613Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34+ CellsDorian Forte0Daria Sollazzo1Martina Barone2Marisole Allegri3Angela di Martella Orsi4Marco Romano5Barbara Sinigaglia6Giuseppe Auteri7Nicola Vianelli8Michele Cavo9Francesca Palandri10Lucia Catani11Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, ItalySchool of Immunology & Microbial Sciences, King’s College London, Guy’s Hospital, SE1 9RT London, UKDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, ItalyInflammation may play a role in cancer. However, the contribution of cytokine-mediated crosstalk between normal hemopoietic stem/progenitor cells (HSPCs) and their (inflammatory) microenvironment is largely elusive. Here we compared survival, phenotype, and function of neonatal (umbilical cord blood (CB)) and adult (normal G-CSF-mobilized peripheral blood (mPB)) CD34+ cells after in vitro exposure to combined crucial inflammatory factors such as interleukin- (IL-) 1β, IL-6, tumor necrosis factor- (TNF-) α, or tissue inhibitor of metalloproteinases-1 (TIMP-1). To mimic bone marrow (BM) niche, coculture experiments with normal BM stromal cells (BMSCs) were also performed. We found that combined inflammatory cytokines increased only the in vitro survival of CB-derived CD34+ cells by reducing apoptosis. Conversely, selected combinations of inflammatory cytokines (IL-1β + TNF-α, IL-6 + TNF-α, and IL-1β + TNF-α + TIMP-1) mainly enhanced the in vitro CXCR4-driven migration of mPB-derived CD34+ cells. TNF-α, alone or in combination, upregulated CD44 and CD13 expression in both sources. Finally, BMSCs alone increased survival/migration of CB- and mPB-derived CD34+ cells at the same extent of the combined inflammatory cytokines; importantly, their copresence did not show additive/synergistic effect. Taken together, these data indicate that combined proinflammatory stimuli promote distinct in vitro functional activation of neonatal or adult normal HSPCs.http://dx.doi.org/10.1155/2018/5974613 |
| spellingShingle | Dorian Forte Daria Sollazzo Martina Barone Marisole Allegri Angela di Martella Orsi Marco Romano Barbara Sinigaglia Giuseppe Auteri Nicola Vianelli Michele Cavo Francesca Palandri Lucia Catani Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34+ Cells Mediators of Inflammation |
| title | Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34+ Cells |
| title_full | Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34+ Cells |
| title_fullStr | Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34+ Cells |
| title_full_unstemmed | Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34+ Cells |
| title_short | Mobilized Peripheral Blood versus Cord Blood: Insight into the Distinct Role of Proinflammatory Cytokines on Survival, Clonogenic Ability, and Migration of CD34+ Cells |
| title_sort | mobilized peripheral blood versus cord blood insight into the distinct role of proinflammatory cytokines on survival clonogenic ability and migration of cd34 cells |
| url | http://dx.doi.org/10.1155/2018/5974613 |
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