Elucidating the potential of bimetallic mixed metal oxide (FeO/NiO) in fusion with pristine and N- and S-doped graphene oxide for biomedical applications

Antimicrobial resistance is attributed to acquiring new mechanisms by microbes to combat antimicrobial agents, highlighting the necessity to discover new antimicrobial agents to protect human health. Graphene and its derivatives have shown antimicrobial potential due to their physical and chemical d...

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Main Authors: Safeena Zafar, Bilal Ahmad Khan, Ikhtiar Ahmad, Muhammad Naeem Ahmed, Aroosa Zafar, Rasool Khan, Mohamed A. El-Tayeb, Ahmed M. Awad, Tamer Shoeib, Mahmoud A.A. Ibrahim
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Chemical Physics Impact
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Online Access:http://www.sciencedirect.com/science/article/pii/S2667022424002925
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author Safeena Zafar
Bilal Ahmad Khan
Ikhtiar Ahmad
Muhammad Naeem Ahmed
Aroosa Zafar
Rasool Khan
Mohamed A. El-Tayeb
Ahmed M. Awad
Tamer Shoeib
Mahmoud A.A. Ibrahim
author_facet Safeena Zafar
Bilal Ahmad Khan
Ikhtiar Ahmad
Muhammad Naeem Ahmed
Aroosa Zafar
Rasool Khan
Mohamed A. El-Tayeb
Ahmed M. Awad
Tamer Shoeib
Mahmoud A.A. Ibrahim
author_sort Safeena Zafar
collection DOAJ
description Antimicrobial resistance is attributed to acquiring new mechanisms by microbes to combat antimicrobial agents, highlighting the necessity to discover new antimicrobial agents to protect human health. Graphene and its derivatives have shown antimicrobial potential due to their physical and chemical distinctive features. Potent antibacterial properties were observed by decorating the surface of graphene and its derivatives with inorganic nanoparticles, such as metal and metal oxide. In an attempt to reliably overcome antimicrobial resistance, the multifunctional nanocomposites, including FeO/NiO, FeO/NiO/GO, FeO/NiO/N-GO, and FeO/NiO/S-GO, were synthesized using a wet chemical method. Accordingly, the structural analysis was performed using X-ray diffraction (XRD), infrared spectroscopy (IR), energy dispersive X-ray (EDX), ultraviolet-visible spectroscopy (UV–vis), and scanning electron microscopy (SEM). For antibacterial potential, the synthesized nanocomposites were tested against non-resistant and resistant strains of bacteria. Notably, moderate antibacterial potential was found for FeO/NiO/N-GO nanocomposite with a MIC value of 12.5 μg/mL, compared to the MIC of pure Ciprofloxacin, a positive control, with a value of 1.25 μg/mL. Toward antifungal potential, the synthesized nanocomposites were assessed against various spores of fungal strains. In this regard, the synthesized nanocomposites were demonstrated as potent antifungal agents. Among the synthesized nanocomposites, FeO/NiO and FeO/NiO/S-GO exhibited the highest ZOI against Aspergillus flavus. Additionally, the activity of these nanocomposites was evaluated by means of total reducing power (TRP), total antioxidant capacity (TAC), and free radical scavenging. Further, the antioxidant, brine shrimp lethality, and hemolytic potential of the synthesized nanocomposites were evaluated to compare their effectiveness. According to brine shrimp lethality, all synthesized nanocomposites were sufficiently active, with a calculated median lethal concentration (LC50) showing ≥ 50 % mortality. The obtained results provide a promising base for the incorporation of nanocomposites in pharmaceutical and biomedical products.
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spelling doaj-art-572a3e252c0a4f95920f09d64656d7232025-08-20T02:48:39ZengElsevierChemical Physics Impact2667-02242024-12-01910074810.1016/j.chphi.2024.100748Elucidating the potential of bimetallic mixed metal oxide (FeO/NiO) in fusion with pristine and N- and S-doped graphene oxide for biomedical applicationsSafeena Zafar0Bilal Ahmad Khan1Ikhtiar Ahmad2Muhammad Naeem Ahmed3Aroosa Zafar4Rasool Khan5Mohamed A. El-Tayeb6Ahmed M. Awad7Tamer Shoeib8Mahmoud A.A. Ibrahim9Department of Chemistry, University of Azad Jammu and Kashmir, Muzaffarabad 13100, PakistanDepartment of Chemistry, University of Azad Jammu and Kashmir, Muzaffarabad 13100, Pakistan; Corresponding authors.Department of Chemistry, University of Azad Jammu and Kashmir, Muzaffarabad 13100, Pakistan; Department of Chemistry, Women University of Azad Jammu & Kashmir, Bagh 12500, PakistanDepartment of Chemistry, University of Azad Jammu and Kashmir, Muzaffarabad 13100, PakistanDepartment of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan; Cadson College of Pharmacy, Kharian 50090, PakistanInstitute of Chemical Sciences, University of Peshawar, Peshawar 25120, PakistanDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Chemistry, California State University Channel Islands, Camarillo, CA 93012, USADepartment of Chemistry, The American University in Cairo, New Cairo 11835, EgyptChemistry Department, Faculty of Science, Minia University, Minia 61519, Egypt; School of Health Sciences, University of KwaZulu-Natal, Westville Campus, Durban 4000, SC Africa; Corresponding authors.Antimicrobial resistance is attributed to acquiring new mechanisms by microbes to combat antimicrobial agents, highlighting the necessity to discover new antimicrobial agents to protect human health. Graphene and its derivatives have shown antimicrobial potential due to their physical and chemical distinctive features. Potent antibacterial properties were observed by decorating the surface of graphene and its derivatives with inorganic nanoparticles, such as metal and metal oxide. In an attempt to reliably overcome antimicrobial resistance, the multifunctional nanocomposites, including FeO/NiO, FeO/NiO/GO, FeO/NiO/N-GO, and FeO/NiO/S-GO, were synthesized using a wet chemical method. Accordingly, the structural analysis was performed using X-ray diffraction (XRD), infrared spectroscopy (IR), energy dispersive X-ray (EDX), ultraviolet-visible spectroscopy (UV–vis), and scanning electron microscopy (SEM). For antibacterial potential, the synthesized nanocomposites were tested against non-resistant and resistant strains of bacteria. Notably, moderate antibacterial potential was found for FeO/NiO/N-GO nanocomposite with a MIC value of 12.5 μg/mL, compared to the MIC of pure Ciprofloxacin, a positive control, with a value of 1.25 μg/mL. Toward antifungal potential, the synthesized nanocomposites were assessed against various spores of fungal strains. In this regard, the synthesized nanocomposites were demonstrated as potent antifungal agents. Among the synthesized nanocomposites, FeO/NiO and FeO/NiO/S-GO exhibited the highest ZOI against Aspergillus flavus. Additionally, the activity of these nanocomposites was evaluated by means of total reducing power (TRP), total antioxidant capacity (TAC), and free radical scavenging. Further, the antioxidant, brine shrimp lethality, and hemolytic potential of the synthesized nanocomposites were evaluated to compare their effectiveness. According to brine shrimp lethality, all synthesized nanocomposites were sufficiently active, with a calculated median lethal concentration (LC50) showing ≥ 50 % mortality. The obtained results provide a promising base for the incorporation of nanocomposites in pharmaceutical and biomedical products.http://www.sciencedirect.com/science/article/pii/S2667022424002925Graphene oxideNanocompositesBimetallic mixed metal oxideAntibacterial resistanceXRD
spellingShingle Safeena Zafar
Bilal Ahmad Khan
Ikhtiar Ahmad
Muhammad Naeem Ahmed
Aroosa Zafar
Rasool Khan
Mohamed A. El-Tayeb
Ahmed M. Awad
Tamer Shoeib
Mahmoud A.A. Ibrahim
Elucidating the potential of bimetallic mixed metal oxide (FeO/NiO) in fusion with pristine and N- and S-doped graphene oxide for biomedical applications
Chemical Physics Impact
Graphene oxide
Nanocomposites
Bimetallic mixed metal oxide
Antibacterial resistance
XRD
title Elucidating the potential of bimetallic mixed metal oxide (FeO/NiO) in fusion with pristine and N- and S-doped graphene oxide for biomedical applications
title_full Elucidating the potential of bimetallic mixed metal oxide (FeO/NiO) in fusion with pristine and N- and S-doped graphene oxide for biomedical applications
title_fullStr Elucidating the potential of bimetallic mixed metal oxide (FeO/NiO) in fusion with pristine and N- and S-doped graphene oxide for biomedical applications
title_full_unstemmed Elucidating the potential of bimetallic mixed metal oxide (FeO/NiO) in fusion with pristine and N- and S-doped graphene oxide for biomedical applications
title_short Elucidating the potential of bimetallic mixed metal oxide (FeO/NiO) in fusion with pristine and N- and S-doped graphene oxide for biomedical applications
title_sort elucidating the potential of bimetallic mixed metal oxide feo nio in fusion with pristine and n and s doped graphene oxide for biomedical applications
topic Graphene oxide
Nanocomposites
Bimetallic mixed metal oxide
Antibacterial resistance
XRD
url http://www.sciencedirect.com/science/article/pii/S2667022424002925
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