Full genome characterization of a Kenyan G8P[14] rotavirus strain suggests artiodactyl-to-human zoonotic transmission
Abstract Background Rotavirus infections are a major cause of severe gastroenteritis in children. Human rotavirus strains with the unconventional G8P[14] genotype have sporadically been detected in diarrheic patients in different parts of the world. However, full genomes of only two human G8P[14] st...
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BMC
2025-06-01
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| Series: | Tropical Medicine and Health |
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| Online Access: | https://doi.org/10.1186/s41182-025-00759-9 |
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| author | Ernest Apondi Wandera Yuki Akari Carlene Sang Pamela Njugu Samoel Ashimosi Khamadi Sebastian Musundi Maurine Mumo Mutua Saori Fukuda Takayuki Murata Shingo Inoue Satoshi Kaneko James Nyangao Satoshi Komoto |
| author_facet | Ernest Apondi Wandera Yuki Akari Carlene Sang Pamela Njugu Samoel Ashimosi Khamadi Sebastian Musundi Maurine Mumo Mutua Saori Fukuda Takayuki Murata Shingo Inoue Satoshi Kaneko James Nyangao Satoshi Komoto |
| author_sort | Ernest Apondi Wandera |
| collection | DOAJ |
| description | Abstract Background Rotavirus infections are a major cause of severe gastroenteritis in children. Human rotavirus strains with the unconventional G8P[14] genotype have sporadically been detected in diarrheic patients in different parts of the world. However, full genomes of only two human G8P[14] strains from Africa (North Africa) have been sequenced, and the origin and evolutionary patterns of African G8P[14] strains remain to be elucidated. Methods In this study, we sequenced the full genome of an African G8P[14] strain (RVA/Human-wt/KEN/A75/2000/G8P[14]) identified in archival stool samples from a diarrheic child in Kenya. Results Full genome-based analysis of strain A75 revealed a unique genogroup constellation, G8-P[14]-I2-R2-C2-M2-A11-N2-T6-E2-H3, with the I2-R2-C2-M2-A11-N2-T6-E2-H3 part being common among rotavirus strains from artiodactyls such as cattle. Phylogenetic analysis showed that all the 11 genomic segments of strain A75 are closely related to segments found in artiodactyl rotavirus strains, and likely strain A75 derived from spillover transmission of an artiodactyl rotavirus strain to humans. Conclusion This is the first report on a full genome-based characterization of a human G8P[14] strain from East Africa. This study demonstrates the diversity of human G8P[14] strains in Africa and contributes to the elucidation of their spreading and evolution, which includes zoonotic transmission from artiodactyls. |
| format | Article |
| id | doaj-art-5713e17a4cbc4a8b926208ee7e7181f8 |
| institution | Kabale University |
| issn | 1349-4147 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Tropical Medicine and Health |
| spelling | doaj-art-5713e17a4cbc4a8b926208ee7e7181f82025-08-20T03:47:17ZengBMCTropical Medicine and Health1349-41472025-06-0153111310.1186/s41182-025-00759-9Full genome characterization of a Kenyan G8P[14] rotavirus strain suggests artiodactyl-to-human zoonotic transmissionErnest Apondi Wandera0Yuki Akari1Carlene Sang2Pamela Njugu3Samoel Ashimosi Khamadi4Sebastian Musundi5Maurine Mumo Mutua6Saori Fukuda7Takayuki Murata8Shingo Inoue9Satoshi Kaneko10James Nyangao11Satoshi Komoto12Centre for Virus Research, Kenya Medical Research Institute (KEMRI)Division of One Health, Research Center for GLOBAL and LOCAL Infectious Diseases (RCGLID), Oita UniversityCentre for Virus Research, Kenya Medical Research Institute (KEMRI)Centre for Virus Research, Kenya Medical Research Institute (KEMRI)Centre for Virus Research, Kenya Medical Research Institute (KEMRI)KEMRI Wellcome Trust Research Programme, KEMRIKenya Research Station, Nagasaki University Institute of Tropical Medicine (NUITM)-KEMRIDepartment of Virology, Fujita Health University School of MedicineDepartment of Virology, Fujita Health University School of MedicineKenya Research Station, Nagasaki University Institute of Tropical Medicine (NUITM)-KEMRIKenya Research Station, Nagasaki University Institute of Tropical Medicine (NUITM)-KEMRICentre for Virus Research, Kenya Medical Research Institute (KEMRI)Division of One Health, Research Center for GLOBAL and LOCAL Infectious Diseases (RCGLID), Oita UniversityAbstract Background Rotavirus infections are a major cause of severe gastroenteritis in children. Human rotavirus strains with the unconventional G8P[14] genotype have sporadically been detected in diarrheic patients in different parts of the world. However, full genomes of only two human G8P[14] strains from Africa (North Africa) have been sequenced, and the origin and evolutionary patterns of African G8P[14] strains remain to be elucidated. Methods In this study, we sequenced the full genome of an African G8P[14] strain (RVA/Human-wt/KEN/A75/2000/G8P[14]) identified in archival stool samples from a diarrheic child in Kenya. Results Full genome-based analysis of strain A75 revealed a unique genogroup constellation, G8-P[14]-I2-R2-C2-M2-A11-N2-T6-E2-H3, with the I2-R2-C2-M2-A11-N2-T6-E2-H3 part being common among rotavirus strains from artiodactyls such as cattle. Phylogenetic analysis showed that all the 11 genomic segments of strain A75 are closely related to segments found in artiodactyl rotavirus strains, and likely strain A75 derived from spillover transmission of an artiodactyl rotavirus strain to humans. Conclusion This is the first report on a full genome-based characterization of a human G8P[14] strain from East Africa. This study demonstrates the diversity of human G8P[14] strains in Africa and contributes to the elucidation of their spreading and evolution, which includes zoonotic transmission from artiodactyls.https://doi.org/10.1186/s41182-025-00759-9Group A rotavirusFull genome-based analysisG8P[14] strainsArtiodactylsInterspecies transmissionKenya |
| spellingShingle | Ernest Apondi Wandera Yuki Akari Carlene Sang Pamela Njugu Samoel Ashimosi Khamadi Sebastian Musundi Maurine Mumo Mutua Saori Fukuda Takayuki Murata Shingo Inoue Satoshi Kaneko James Nyangao Satoshi Komoto Full genome characterization of a Kenyan G8P[14] rotavirus strain suggests artiodactyl-to-human zoonotic transmission Tropical Medicine and Health Group A rotavirus Full genome-based analysis G8P[14] strains Artiodactyls Interspecies transmission Kenya |
| title | Full genome characterization of a Kenyan G8P[14] rotavirus strain suggests artiodactyl-to-human zoonotic transmission |
| title_full | Full genome characterization of a Kenyan G8P[14] rotavirus strain suggests artiodactyl-to-human zoonotic transmission |
| title_fullStr | Full genome characterization of a Kenyan G8P[14] rotavirus strain suggests artiodactyl-to-human zoonotic transmission |
| title_full_unstemmed | Full genome characterization of a Kenyan G8P[14] rotavirus strain suggests artiodactyl-to-human zoonotic transmission |
| title_short | Full genome characterization of a Kenyan G8P[14] rotavirus strain suggests artiodactyl-to-human zoonotic transmission |
| title_sort | full genome characterization of a kenyan g8p 14 rotavirus strain suggests artiodactyl to human zoonotic transmission |
| topic | Group A rotavirus Full genome-based analysis G8P[14] strains Artiodactyls Interspecies transmission Kenya |
| url | https://doi.org/10.1186/s41182-025-00759-9 |
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