Generation of an integration-free induced pluripotent stem cell line, MURAi006-A, from a hemoglobin E/β-thalassemia patient harboring the βE/β0 (Codon 17, A > T) compound heterozygous mutation
The HBB gene encodes the β-globin protein, one of the two main components of adult hemoglobin A (HbA) responsible for oxygen transport. β-thalassemia is a genetic disorder caused by mutations affecting β-globin chain synthesis, leading to reduced or absent β-globin production, impaired erythropoiesi...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506125000522 |
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| author | Pawarit Innachai Gunn Pornratananont Chonthicha Satirapod Usanarat Anurathapan Duantida Songdej Amornrat Tangprasittipap Suradej Hongeng |
| author_facet | Pawarit Innachai Gunn Pornratananont Chonthicha Satirapod Usanarat Anurathapan Duantida Songdej Amornrat Tangprasittipap Suradej Hongeng |
| author_sort | Pawarit Innachai |
| collection | DOAJ |
| description | The HBB gene encodes the β-globin protein, one of the two main components of adult hemoglobin A (HbA) responsible for oxygen transport. β-thalassemia is a genetic disorder caused by mutations affecting β-globin chain synthesis, leading to reduced or absent β-globin production, impaired erythropoiesis, and generally results in anemia. In this study, the human-induced pluripotent stem cell line (hiPSC) MURAi006-A was generated from male fetal skin fibroblasts carrying both a β⁰-thalassemia mutation at codon 17 (A > T) and a codon 26 (G > A) HbE mutation using non-integrative reprogramming episomes. |
| format | Article |
| id | doaj-art-570e36da5b1c4352bbaabe3797da64db |
| institution | DOAJ |
| issn | 1873-5061 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-570e36da5b1c4352bbaabe3797da64db2025-08-20T03:14:45ZengElsevierStem Cell Research1873-50612025-06-018510370210.1016/j.scr.2025.103702Generation of an integration-free induced pluripotent stem cell line, MURAi006-A, from a hemoglobin E/β-thalassemia patient harboring the βE/β0 (Codon 17, A > T) compound heterozygous mutationPawarit Innachai0Gunn Pornratananont1Chonthicha Satirapod2Usanarat Anurathapan3Duantida Songdej4Amornrat Tangprasittipap5Suradej Hongeng6Offices of Health Science Research, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandProgram in Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDepartment of Obstetrics and Gynecology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, ThailandDepartment of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandDepartment of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandOffices of Health Science Research, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand; Corresponding author.Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok 10400, ThailandThe HBB gene encodes the β-globin protein, one of the two main components of adult hemoglobin A (HbA) responsible for oxygen transport. β-thalassemia is a genetic disorder caused by mutations affecting β-globin chain synthesis, leading to reduced or absent β-globin production, impaired erythropoiesis, and generally results in anemia. In this study, the human-induced pluripotent stem cell line (hiPSC) MURAi006-A was generated from male fetal skin fibroblasts carrying both a β⁰-thalassemia mutation at codon 17 (A > T) and a codon 26 (G > A) HbE mutation using non-integrative reprogramming episomes.http://www.sciencedirect.com/science/article/pii/S1873506125000522 |
| spellingShingle | Pawarit Innachai Gunn Pornratananont Chonthicha Satirapod Usanarat Anurathapan Duantida Songdej Amornrat Tangprasittipap Suradej Hongeng Generation of an integration-free induced pluripotent stem cell line, MURAi006-A, from a hemoglobin E/β-thalassemia patient harboring the βE/β0 (Codon 17, A > T) compound heterozygous mutation Stem Cell Research |
| title | Generation of an integration-free induced pluripotent stem cell line, MURAi006-A, from a hemoglobin E/β-thalassemia patient harboring the βE/β0 (Codon 17, A > T) compound heterozygous mutation |
| title_full | Generation of an integration-free induced pluripotent stem cell line, MURAi006-A, from a hemoglobin E/β-thalassemia patient harboring the βE/β0 (Codon 17, A > T) compound heterozygous mutation |
| title_fullStr | Generation of an integration-free induced pluripotent stem cell line, MURAi006-A, from a hemoglobin E/β-thalassemia patient harboring the βE/β0 (Codon 17, A > T) compound heterozygous mutation |
| title_full_unstemmed | Generation of an integration-free induced pluripotent stem cell line, MURAi006-A, from a hemoglobin E/β-thalassemia patient harboring the βE/β0 (Codon 17, A > T) compound heterozygous mutation |
| title_short | Generation of an integration-free induced pluripotent stem cell line, MURAi006-A, from a hemoglobin E/β-thalassemia patient harboring the βE/β0 (Codon 17, A > T) compound heterozygous mutation |
| title_sort | generation of an integration free induced pluripotent stem cell line murai006 a from a hemoglobin e β thalassemia patient harboring the βe β0 codon 17 a t compound heterozygous mutation |
| url | http://www.sciencedirect.com/science/article/pii/S1873506125000522 |
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