Genome-wide identification and analysis of potassium ion transporter HAK/KUP/KT family in rapeseed (Brassica napus L.)
The high-affinity K<sup>＋</sup>/K<sup>＋</sup> uptake permease/K<sup>＋</sup> transporter (HAK/KUP/KT) family members play important roles in the absorption and transport of potassium (K), regulations of plant growth and development, modulation/acquisition of salt t...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2021-06-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2020.08.251 |
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| Summary: | The high-affinity K<sup>＋</sup>/K<sup>＋</sup> uptake permease/K<sup>＋</sup> transporter (HAK/KUP/KT) family members play important roles in the absorption and transport of potassium (K), regulations of plant growth and development, modulation/acquisition of salt tolerance, and osmotic potential regulation/control. However, this gene family has not been thoroughly studied in Brassica napus. In this study, forty-five BnHAK genes (BnHAK1-BnHAK45) were identified in the B. napus reference genome, and bioinformatic methods were used to resolve the gene structures, evolutionary analysis, expression patterns and chromosome position in different tissues of rape. The results showed that the members of the HAK/KUP/KT gene family were unevenly distributed on the chromosomes, with the most of them located on chromosome A01 which had five BnHAK genes. Phylogenetic analysis of HAK/KUP/KT proteins from multiple species showed that they could be divided into two groups. Among them, Arabidopsis, B. oleracea, B. rapa and B. napus had stronger gene conservation and closer genetic relationships. Although the expression patterns of the HAK/KUP/KT genes were different, in general, they exhibited highest and lowest expression levels in the roots and siliques, respectively, and this showed that there was a functional division of different HAK/KUP/KT gene family members. The expression levels of BnHAK4 and BnHAK9 were highest in the leaves, whilst the expression levels of BnHAK11, BnHAK18, and BnHAK23 were highest in the roots, and BnHAK7 and BnHAK17 were mostly expressed in the flowers, which indicated that the expression of this gene family had tissue specificity. This study provides a reference for further revealing the functional mechanism of HAK/KUP/KT gene family in B. napus. |
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| ISSN: | 1008-9209 2097-5155 |