Transcriptional alteration of NF-κB-associated long noncoding RNAs in the stomach of Helicobacter pylori-infected and non-infected patients
Introduction: Helicobacter pylori could colonize the gastric mucosa and cause gastritis, ulcers and cancer. Numerous virulence factors have been identified in this bacterium that play important roles in promoting gastric disorders. Although the interaction of long noncoding RNAs (lncRNAs) with tran...
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The Journal of Infection in Developing Countries
2023-11-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/17748 |
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| author | Marzieh Ghanbarian Ronak Bakhtiari Seyedeh Zohre Mirbagheri Fatemeh Rezaei Abbas Rahimi Foroushani Masoud Alebouyeh |
| author_facet | Marzieh Ghanbarian Ronak Bakhtiari Seyedeh Zohre Mirbagheri Fatemeh Rezaei Abbas Rahimi Foroushani Masoud Alebouyeh |
| author_sort | Marzieh Ghanbarian |
| collection | DOAJ |
| description |
Introduction: Helicobacter pylori could colonize the gastric mucosa and cause gastritis, ulcers and cancer. Numerous virulence factors have been identified in this bacterium that play important roles in promoting gastric disorders. Although the interaction of long noncoding RNAs (lncRNAs) with transcription, processing, and translation of genes associated with different diseases are described, their interaction with the inflammatory genes and H. pylori infection in the gastric tissue is not well known. This study compared changes in common NF-κB-regulatory lncRNAs in the gastric tissue of H. pylori-infected and non-infected patients with gastritis. Moreover, a link between the virulence entity of the strains and the transcriptional changes was analyzed.
Methodology: Two groups of infected and non-infected patients with chronic gastritis were included in the study. Genotyping of the H. pylori strains was done by PCR and relative changes in the expression of NF-κB and regulatory lncRNAs, lincRNA-p21, MALAT1, NKILA, were measured by relative quantitative real time-PCR.
Results: Transcriptional levels of MALAT1, lincRNA-p21, and NKILA genes decreased in the infected patients compared with the non-infected patients, which was significantly linked with increased NF-κB gene expression. Our results showed that a hypervirulent strain of H. pylori with oipA“on”/HP-NAP+/iceA1+/iceA2+/vacA s1m1/s1m2+/cagA+ genotype can promote a higher level of NF-κB transcription in the inflamed tissue.
Conclusions: H. pylori infection could promote down-regulation of lincRNA-p21, MALAT1 and NKILA in the infected gastric tissue in correlation with NF-κB upregulation. More detailed studies are needed to show a link between the virulence genes and their impact on the regulation of lncRNAs in the stomach.
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| format | Article |
| id | doaj-art-56f6bf5fdf8742cf88cd4aae32ef61c1 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-56f6bf5fdf8742cf88cd4aae32ef61c12025-08-20T02:57:13ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802023-11-01171110.3855/jidc.17748Transcriptional alteration of NF-κB-associated long noncoding RNAs in the stomach of Helicobacter pylori-infected and non-infected patientsMarzieh Ghanbarian0Ronak Bakhtiari1Seyedeh Zohre Mirbagheri2Fatemeh Rezaei3Abbas Rahimi Foroushani4Masoud Alebouyeh5Department of Pathobiology, School of Public Health and Institute Health Research, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathobiology, School of Public Health and Institute Health Research, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathobiology, School of Public Health and Institute Health Research, Tehran University of Medical Sciences, Tehran, IranDepartment of Pathobiology, School of Public Health and Institute Health Research, Tehran University of Medical Sciences, Tehran, IranDepartment of Epidemiology and Biostatistics, School of Public Health and Institute Health Research, Tehran University of Medical Sciences, Tehran, IranPediatric Infections Research Centre, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran Introduction: Helicobacter pylori could colonize the gastric mucosa and cause gastritis, ulcers and cancer. Numerous virulence factors have been identified in this bacterium that play important roles in promoting gastric disorders. Although the interaction of long noncoding RNAs (lncRNAs) with transcription, processing, and translation of genes associated with different diseases are described, their interaction with the inflammatory genes and H. pylori infection in the gastric tissue is not well known. This study compared changes in common NF-κB-regulatory lncRNAs in the gastric tissue of H. pylori-infected and non-infected patients with gastritis. Moreover, a link between the virulence entity of the strains and the transcriptional changes was analyzed. Methodology: Two groups of infected and non-infected patients with chronic gastritis were included in the study. Genotyping of the H. pylori strains was done by PCR and relative changes in the expression of NF-κB and regulatory lncRNAs, lincRNA-p21, MALAT1, NKILA, were measured by relative quantitative real time-PCR. Results: Transcriptional levels of MALAT1, lincRNA-p21, and NKILA genes decreased in the infected patients compared with the non-infected patients, which was significantly linked with increased NF-κB gene expression. Our results showed that a hypervirulent strain of H. pylori with oipA“on”/HP-NAP+/iceA1+/iceA2+/vacA s1m1/s1m2+/cagA+ genotype can promote a higher level of NF-κB transcription in the inflamed tissue. Conclusions: H. pylori infection could promote down-regulation of lincRNA-p21, MALAT1 and NKILA in the infected gastric tissue in correlation with NF-κB upregulation. More detailed studies are needed to show a link between the virulence genes and their impact on the regulation of lncRNAs in the stomach. https://jidc.org/index.php/journal/article/view/17748Helicobacter pylorilncRNANF-κBchronic gastritis |
| spellingShingle | Marzieh Ghanbarian Ronak Bakhtiari Seyedeh Zohre Mirbagheri Fatemeh Rezaei Abbas Rahimi Foroushani Masoud Alebouyeh Transcriptional alteration of NF-κB-associated long noncoding RNAs in the stomach of Helicobacter pylori-infected and non-infected patients Journal of Infection in Developing Countries Helicobacter pylori lncRNA NF-κB chronic gastritis |
| title | Transcriptional alteration of NF-κB-associated long noncoding RNAs in the stomach of Helicobacter pylori-infected and non-infected patients |
| title_full | Transcriptional alteration of NF-κB-associated long noncoding RNAs in the stomach of Helicobacter pylori-infected and non-infected patients |
| title_fullStr | Transcriptional alteration of NF-κB-associated long noncoding RNAs in the stomach of Helicobacter pylori-infected and non-infected patients |
| title_full_unstemmed | Transcriptional alteration of NF-κB-associated long noncoding RNAs in the stomach of Helicobacter pylori-infected and non-infected patients |
| title_short | Transcriptional alteration of NF-κB-associated long noncoding RNAs in the stomach of Helicobacter pylori-infected and non-infected patients |
| title_sort | transcriptional alteration of nf κb associated long noncoding rnas in the stomach of helicobacter pylori infected and non infected patients |
| topic | Helicobacter pylori lncRNA NF-κB chronic gastritis |
| url | https://jidc.org/index.php/journal/article/view/17748 |
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