Molecular diversity of Mycobacterium tuberculosis isolates in Treatment-experienced Patients from Andhra Pradesh, India
Introduction: To get a comprehensive idea about the transmission and epidemiology of TB globally and locally, the use of molecular typing methods has become imperative not only for understanding genetic diversity but also the population structure of Mycobacterium tuberculosis complex (MTBC). We aim...
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The Journal of Infection in Developing Countries
2023-08-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/17757 |
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| author | Anagoni Srikar Banda Venkataramana Alladi Mohan PVGK Sarma Kangjam Rekha Devi Kanwar Narain Abhijit Chaudhury |
| author_facet | Anagoni Srikar Banda Venkataramana Alladi Mohan PVGK Sarma Kangjam Rekha Devi Kanwar Narain Abhijit Chaudhury |
| author_sort | Anagoni Srikar |
| collection | DOAJ |
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Introduction: To get a comprehensive idea about the transmission and epidemiology of TB globally and locally, the use of molecular typing methods has become imperative not only for understanding genetic diversity but also the population structure of Mycobacterium tuberculosis complex (MTBC). We aimed to investigate the drug resistance pattern and genetic diversity of MTBC among previously treated patients with sputum smear-positive pulmonary tuberculosis in a South Indian population.
Methodology: 104 patients with sputum smear positivity and who had previously undergone treatment were selected. Drug susceptibility testing, Spoligotyping, MIRU-VNTR, and SNP typing were performed.
Results: Mono-resistance to isoniazid 16 (15.38%) was the highest among all drugs. Out of 104 isolates, 24 (23%) isolates were classified as MDR strains. The distributions of most common lineages were: EAI3-Ind–20 (19.23%), EAI5-13 (12.50%), Beijing-12 (11.54%), CAS1-Delhi- 9 (8.65%), and 7 (6.73%) each of T-H37rv, Unknown and Orphan types. MIRU-VNTR-based analysis revealed that there are two major groups: CAS1-Delhi and Beijing groups. Out of 104 isolates, 82 belonged to well-defined lineages and 6 clusters, and the remaining 22 were singletons. SNP analysis showed no mutations associated with five sets of genes in 33 strains.
Conclusions: The occurrence of 11.54% Beijing strains in South India is an important finding. High frequency of Isoniazid mono resistance noticed. Spoligotyping along with MIRU-VNTR and SNP typing is the best approach to the identification of strain lineages. No mutation with Antigen85C gene represents, can be used for vaccine candidates.
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| format | Article |
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| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2023-08-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-56f103b57bca41fc9ea8c1380df4c07f2025-08-20T02:27:14ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802023-08-01170810.3855/jidc.17757Molecular diversity of Mycobacterium tuberculosis isolates in Treatment-experienced Patients from Andhra Pradesh, IndiaAnagoni Srikar0Banda Venkataramana1Alladi Mohan2PVGK Sarma3Kangjam Rekha Devi4Kanwar Narain5Abhijit Chaudhury6Department of Microbiology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, IndiaDepartment of Microbiology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, IndiaDepartment of Medicine, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, IndiaDepartment of Biotechnology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, IndiaDepartment of Enteric diseases, Regional Medical Research Centre, Dibrugarh, Assam, IndiaDepartment of Enteric diseases, Regional Medical Research Centre, Dibrugarh, Assam, IndiaDepartment of Microbiology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, India Introduction: To get a comprehensive idea about the transmission and epidemiology of TB globally and locally, the use of molecular typing methods has become imperative not only for understanding genetic diversity but also the population structure of Mycobacterium tuberculosis complex (MTBC). We aimed to investigate the drug resistance pattern and genetic diversity of MTBC among previously treated patients with sputum smear-positive pulmonary tuberculosis in a South Indian population. Methodology: 104 patients with sputum smear positivity and who had previously undergone treatment were selected. Drug susceptibility testing, Spoligotyping, MIRU-VNTR, and SNP typing were performed. Results: Mono-resistance to isoniazid 16 (15.38%) was the highest among all drugs. Out of 104 isolates, 24 (23%) isolates were classified as MDR strains. The distributions of most common lineages were: EAI3-Ind–20 (19.23%), EAI5-13 (12.50%), Beijing-12 (11.54%), CAS1-Delhi- 9 (8.65%), and 7 (6.73%) each of T-H37rv, Unknown and Orphan types. MIRU-VNTR-based analysis revealed that there are two major groups: CAS1-Delhi and Beijing groups. Out of 104 isolates, 82 belonged to well-defined lineages and 6 clusters, and the remaining 22 were singletons. SNP analysis showed no mutations associated with five sets of genes in 33 strains. Conclusions: The occurrence of 11.54% Beijing strains in South India is an important finding. High frequency of Isoniazid mono resistance noticed. Spoligotyping along with MIRU-VNTR and SNP typing is the best approach to the identification of strain lineages. No mutation with Antigen85C gene represents, can be used for vaccine candidates. https://jidc.org/index.php/journal/article/view/17757Drug resistancegenetic diversityMIRU-VNTR typingMycobacterium tuberculosisSpoligo-international type (SIT)SNP typing |
| spellingShingle | Anagoni Srikar Banda Venkataramana Alladi Mohan PVGK Sarma Kangjam Rekha Devi Kanwar Narain Abhijit Chaudhury Molecular diversity of Mycobacterium tuberculosis isolates in Treatment-experienced Patients from Andhra Pradesh, India Journal of Infection in Developing Countries Drug resistance genetic diversity MIRU-VNTR typing Mycobacterium tuberculosis Spoligo-international type (SIT) SNP typing |
| title | Molecular diversity of Mycobacterium tuberculosis isolates in Treatment-experienced Patients from Andhra Pradesh, India |
| title_full | Molecular diversity of Mycobacterium tuberculosis isolates in Treatment-experienced Patients from Andhra Pradesh, India |
| title_fullStr | Molecular diversity of Mycobacterium tuberculosis isolates in Treatment-experienced Patients from Andhra Pradesh, India |
| title_full_unstemmed | Molecular diversity of Mycobacterium tuberculosis isolates in Treatment-experienced Patients from Andhra Pradesh, India |
| title_short | Molecular diversity of Mycobacterium tuberculosis isolates in Treatment-experienced Patients from Andhra Pradesh, India |
| title_sort | molecular diversity of mycobacterium tuberculosis isolates in treatment experienced patients from andhra pradesh india |
| topic | Drug resistance genetic diversity MIRU-VNTR typing Mycobacterium tuberculosis Spoligo-international type (SIT) SNP typing |
| url | https://jidc.org/index.php/journal/article/view/17757 |
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