Cholesterol taste avoidance in Drosophila melanogaster

The question as to whether animals taste cholesterol taste is not resolved. This study investigates whether the fruit fly, Drosophila melanogaster, is capable of detecting cholesterol through their gustatory system. We found that flies are indifferent to low levels of cholesterol and avoid higher le...

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Main Authors: Roshani Nhuchhen Pradhan, Craig Montell, Youngseok Lee
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2025-04-01
Series:eLife
Subjects:
Online Access:https://elifesciences.org/articles/106256
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author Roshani Nhuchhen Pradhan
Craig Montell
Youngseok Lee
author_facet Roshani Nhuchhen Pradhan
Craig Montell
Youngseok Lee
author_sort Roshani Nhuchhen Pradhan
collection DOAJ
description The question as to whether animals taste cholesterol taste is not resolved. This study investigates whether the fruit fly, Drosophila melanogaster, is capable of detecting cholesterol through their gustatory system. We found that flies are indifferent to low levels of cholesterol and avoid higher levels. The avoidance is mediated by gustatory receptor neurons (GRNs), demonstrating that flies can taste cholesterol. The cholesterol-responsive GRNs comprise a subset that also responds to bitter substances. Cholesterol detection depends on five ionotropic receptor (IR) family members, and disrupting any of these genes impairs the flies' ability to avoid cholesterol. Ectopic expressions of these IRs in GRNs reveals two classes of cholesterol receptors, each with three shared IRs and one unique subunit. Additionally, expressing cholesterol receptors in sugar-responsive GRNs confers attraction to cholesterol. This study reveals that flies can taste cholesterol, and that the detection depends on IRs in GRNs.
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spelling doaj-art-56e479a476c948bbafe7cfb1ebec19a82025-08-20T02:27:42ZengeLife Sciences Publications LtdeLife2050-084X2025-04-011410.7554/eLife.106256Cholesterol taste avoidance in Drosophila melanogasterRoshani Nhuchhen Pradhan0https://orcid.org/0000-0001-6772-6357Craig Montell1https://orcid.org/0000-0001-5637-1482Youngseok Lee2https://orcid.org/0000-0003-0459-1138Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul, Republic of KoreaDepartment of Molecular, Cellular, and Developmental Biology, and the Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, United StatesDepartment of Bio and Fermentation Convergence Technology, Kookmin University, Seoul, Republic of KoreaThe question as to whether animals taste cholesterol taste is not resolved. This study investigates whether the fruit fly, Drosophila melanogaster, is capable of detecting cholesterol through their gustatory system. We found that flies are indifferent to low levels of cholesterol and avoid higher levels. The avoidance is mediated by gustatory receptor neurons (GRNs), demonstrating that flies can taste cholesterol. The cholesterol-responsive GRNs comprise a subset that also responds to bitter substances. Cholesterol detection depends on five ionotropic receptor (IR) family members, and disrupting any of these genes impairs the flies' ability to avoid cholesterol. Ectopic expressions of these IRs in GRNs reveals two classes of cholesterol receptors, each with three shared IRs and one unique subunit. Additionally, expressing cholesterol receptors in sugar-responsive GRNs confers attraction to cholesterol. This study reveals that flies can taste cholesterol, and that the detection depends on IRs in GRNs.https://elifesciences.org/articles/106256Cholesteroltastegustatory receptor neuronsIR7gIR51bIR56d
spellingShingle Roshani Nhuchhen Pradhan
Craig Montell
Youngseok Lee
Cholesterol taste avoidance in Drosophila melanogaster
eLife
Cholesterol
taste
gustatory receptor neurons
IR7g
IR51b
IR56d
title Cholesterol taste avoidance in Drosophila melanogaster
title_full Cholesterol taste avoidance in Drosophila melanogaster
title_fullStr Cholesterol taste avoidance in Drosophila melanogaster
title_full_unstemmed Cholesterol taste avoidance in Drosophila melanogaster
title_short Cholesterol taste avoidance in Drosophila melanogaster
title_sort cholesterol taste avoidance in drosophila melanogaster
topic Cholesterol
taste
gustatory receptor neurons
IR7g
IR51b
IR56d
url https://elifesciences.org/articles/106256
work_keys_str_mv AT roshaninhuchhenpradhan cholesteroltasteavoidanceindrosophilamelanogaster
AT craigmontell cholesteroltasteavoidanceindrosophilamelanogaster
AT youngseoklee cholesteroltasteavoidanceindrosophilamelanogaster