Association between single nucleotide polymorphism of immunoregulatory genes and preterm premature rupture of membranes in preterm labour
Aim. Investigation of the association between SNP genes of IL4 (rs2243250), IL10 (rs1800896 and rs1800872) and RLN2 (rs4742076 and rs3758239) and preterm premature rupture of membranes in 26–34 weeks of gestation in Zaporizhzhia population. Materials and methods. We have investigated markers of c...
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Zaporizhzhia State Medical and Pharmaceutical University
2018-09-01
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| Online Access: | http://pat.zsmu.edu.ua/article/view/141400/140079 |
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| author | K. S. Liubomyrska O. M. Kamyshnyi Yu. Ya. Krut |
| author_facet | K. S. Liubomyrska O. M. Kamyshnyi Yu. Ya. Krut |
| author_sort | K. S. Liubomyrska |
| collection | DOAJ |
| description | Aim. Investigation of the association between SNP genes of IL4 (rs2243250), IL10 (rs1800896 and rs1800872) and RLN2 (rs4742076 and rs3758239) and preterm premature rupture of membranes in 26–34 weeks of gestation in Zaporizhzhia population.
Materials and methods. We have investigated markers of cytokines genes in 50 women with PPROM in 26–34 weeks of gestation period and 50 pregnant women with physiological pregnancy and term labour without complications. The genotyping using TaqMan tests was done on amplifier CFX96™ Real-Time PCR Detection Systems (Bio-Rad Laboratories, Inc., USA. The combined effect of the studied locus of the analyzed genes on the appearance of PPROM, PTL in the population was initiated using the Multifactory Dimensionality Reduction (MDR) method (software MDR ver. 3.0.2).
Results. The distribution of the rs2243250 gene polymorphism alleles of the IL4 gene of the main study group – TT homozygotes were determined in 4 % cases, CT heterozygotes were found in 22 %, CC homozygotes – in 74 % cases. We have got statistically significant differences of rs4742076 polymorphisms (TT, CT, and CC) of the RLN2 gene in the study groups. We also detected statistically significant differences in all the alleles of rs3758239 polymorphism (AA, GG and AG) of the gene RLN2 – χ2 = 23.86, OR(AA) = 12.57; 95 % CI: 3.68–42.98; OR(GG)= 0.08; 95 % CI: 0.02–0.27; and OR(AG)= 0.11; 95 % CI: 0.03–0.42, respectively, P < 0.05, indicating the reliability of the received prognostic markers. The nature of interlocal interaction between the genes is at the level of “independent effects” of influence (IL4-IL10 rs1800896 = -23.08 %, IL10 rs1800896 – IL10 rs1800872 = -19.94 %, IL10 rs1800872-IL-4 = -19.34 %). The percentage of entropy of the investigated polymorphism of each gene for the case-control status was 14.15 % for the IL4 gene, 23.08 % for the IL10 rs1800896 gene and 9.34 % for the IL10 rs1800872 gene.
Conclusions. Combination of IL4 rs2243250, IL10 rs1800896 and rs1800872 supports the role for functional polymorphisms in immunoregulatory genes in the development of PPROM and PTL. Reliable clinical association of rs4742076 and rs3758239 gene RLN2 was established with the PPROM in 26–34 weeks’ gestation in Zaporizhzhia population. |
| format | Article |
| id | doaj-art-56e32485a76149fbbe2c5f3cd4db06f8 |
| institution | Kabale University |
| issn | 2306-8027 2310-1237 |
| language | English |
| publishDate | 2018-09-01 |
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| spelling | doaj-art-56e32485a76149fbbe2c5f3cd4db06f82025-08-20T03:33:35ZengZaporizhzhia State Medical and Pharmaceutical UniversityPatologìâ2306-80272310-12372018-09-01218719310.14739/2310-1237.2018.2.141400Association between single nucleotide polymorphism of immunoregulatory genes and preterm premature rupture of membranes in preterm labourK. S. LiubomyrskaO. M. KamyshnyiYu. Ya. KrutAim. Investigation of the association between SNP genes of IL4 (rs2243250), IL10 (rs1800896 and rs1800872) and RLN2 (rs4742076 and rs3758239) and preterm premature rupture of membranes in 26–34 weeks of gestation in Zaporizhzhia population. Materials and methods. We have investigated markers of cytokines genes in 50 women with PPROM in 26–34 weeks of gestation period and 50 pregnant women with physiological pregnancy and term labour without complications. The genotyping using TaqMan tests was done on amplifier CFX96™ Real-Time PCR Detection Systems (Bio-Rad Laboratories, Inc., USA. The combined effect of the studied locus of the analyzed genes on the appearance of PPROM, PTL in the population was initiated using the Multifactory Dimensionality Reduction (MDR) method (software MDR ver. 3.0.2). Results. The distribution of the rs2243250 gene polymorphism alleles of the IL4 gene of the main study group – TT homozygotes were determined in 4 % cases, CT heterozygotes were found in 22 %, CC homozygotes – in 74 % cases. We have got statistically significant differences of rs4742076 polymorphisms (TT, CT, and CC) of the RLN2 gene in the study groups. We also detected statistically significant differences in all the alleles of rs3758239 polymorphism (AA, GG and AG) of the gene RLN2 – χ2 = 23.86, OR(AA) = 12.57; 95 % CI: 3.68–42.98; OR(GG)= 0.08; 95 % CI: 0.02–0.27; and OR(AG)= 0.11; 95 % CI: 0.03–0.42, respectively, P < 0.05, indicating the reliability of the received prognostic markers. The nature of interlocal interaction between the genes is at the level of “independent effects” of influence (IL4-IL10 rs1800896 = -23.08 %, IL10 rs1800896 – IL10 rs1800872 = -19.94 %, IL10 rs1800872-IL-4 = -19.34 %). The percentage of entropy of the investigated polymorphism of each gene for the case-control status was 14.15 % for the IL4 gene, 23.08 % for the IL10 rs1800896 gene and 9.34 % for the IL10 rs1800872 gene. Conclusions. Combination of IL4 rs2243250, IL10 rs1800896 and rs1800872 supports the role for functional polymorphisms in immunoregulatory genes in the development of PPROM and PTL. Reliable clinical association of rs4742076 and rs3758239 gene RLN2 was established with the PPROM in 26–34 weeks’ gestation in Zaporizhzhia population.http://pat.zsmu.edu.ua/article/view/141400/140079preterm labourpreterm premature rupture of membranessingle nucleotide polymorphism |
| spellingShingle | K. S. Liubomyrska O. M. Kamyshnyi Yu. Ya. Krut Association between single nucleotide polymorphism of immunoregulatory genes and preterm premature rupture of membranes in preterm labour Patologìâ preterm labour preterm premature rupture of membranes single nucleotide polymorphism |
| title | Association between single nucleotide polymorphism of immunoregulatory genes and preterm premature rupture of membranes in preterm labour |
| title_full | Association between single nucleotide polymorphism of immunoregulatory genes and preterm premature rupture of membranes in preterm labour |
| title_fullStr | Association between single nucleotide polymorphism of immunoregulatory genes and preterm premature rupture of membranes in preterm labour |
| title_full_unstemmed | Association between single nucleotide polymorphism of immunoregulatory genes and preterm premature rupture of membranes in preterm labour |
| title_short | Association between single nucleotide polymorphism of immunoregulatory genes and preterm premature rupture of membranes in preterm labour |
| title_sort | association between single nucleotide polymorphism of immunoregulatory genes and preterm premature rupture of membranes in preterm labour |
| topic | preterm labour preterm premature rupture of membranes single nucleotide polymorphism |
| url | http://pat.zsmu.edu.ua/article/view/141400/140079 |
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