Virological and genetic characteristics of human respiratory syncytial viruses isolated in Russia, 2017-2018
Isolation of human respiratory syncytial virus (HRSV) from clinical samples and storage of isolates for long period remains a considerable problem. We describe in detail the optimized conditions of HRSV isolation and cultivation in three cell cultures HeLa, HEp-2, and Vero. HRSV was detected in 35....
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The Journal of Infection in Developing Countries
2023-02-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/17462 |
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| author | Elena Alexandrovna Prokopyeva Olga Grigoryevna Kurskaya Mariya Vladimirovna Solomatina Ivan Andreevich Sobolev Tereza Araevna Saroyan Nikita Artemyevich Dubovitskiy Anastasiya Alexandrovna Derko Peter Mikchailovich Kozhin Tatyana Yurievna Alikina Marsel Rasimovich Kabilov Alexander Mikchailovich Shestopalov Kirill Alexandrovich Sharshov |
| author_facet | Elena Alexandrovna Prokopyeva Olga Grigoryevna Kurskaya Mariya Vladimirovna Solomatina Ivan Andreevich Sobolev Tereza Araevna Saroyan Nikita Artemyevich Dubovitskiy Anastasiya Alexandrovna Derko Peter Mikchailovich Kozhin Tatyana Yurievna Alikina Marsel Rasimovich Kabilov Alexander Mikchailovich Shestopalov Kirill Alexandrovich Sharshov |
| author_sort | Elena Alexandrovna Prokopyeva |
| collection | DOAJ |
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Isolation of human respiratory syncytial virus (HRSV) from clinical samples and storage of isolates for long period remains a considerable problem. We describe in detail the optimized conditions of HRSV isolation and cultivation in three cell cultures HeLa, HEp-2, and Vero. HRSV was detected in 35.2% (166/471) specimens by real-time PCR from symptomatic infants and children up to 15 years from October 2017 to March 2018 in Russia. HRSV-positive samples were used for virus isolation in HeLa, HEp-2, and Vero cells in different manners (in monolayer or suspension). To optimize the conditions of HRSV cultivation, these cell cultures were treated or not with receptor-destroying enzyme (RDE). Ten isolates were successfully obtained by the way of infection of the suspension of cells with subsequent RDE treatment. Among them, several isolates induced the cytopathogenic effect (CPE) by the syncytium formation in both Hela and HEp-2 cell cultures. The genetic analysis revealed that the manners of isolation by using monolayer or suspension and subsequent RDE treatment did not influence the nucleotide and amino acid structures of obtained HRSVs. The CPE characteristics of obtained viruses were the same in HeLa, HEp-2, and Vero cell cultures, and were described as large syncytium up to 150 microns or more in size with the nuclei peripheral location and an optically bright zone in the center of the formation. We showed that infection of cell suspension with the subsequent RDE treatment increased the chance of HRSVs isolation from clinical samples.
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| format | Article |
| id | doaj-art-56d85bee4fb74a39ba80aa9f88edf698 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-56d85bee4fb74a39ba80aa9f88edf6982025-08-20T03:48:47ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802023-02-01170210.3855/jidc.17462Virological and genetic characteristics of human respiratory syncytial viruses isolated in Russia, 2017-2018Elena Alexandrovna Prokopyeva0Olga Grigoryevna Kurskaya1Mariya Vladimirovna Solomatina2Ivan Andreevich Sobolev3Tereza Araevna Saroyan4Nikita Artemyevich Dubovitskiy5Anastasiya Alexandrovna Derko6Peter Mikchailovich Kozhin7Tatyana Yurievna Alikina8Marsel Rasimovich Kabilov9Alexander Mikchailovich Shestopalov10Kirill Alexandrovich Sharshov11Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, RussiaFederal Research Center of Fundamental and Translational Medicine, Novosibirsk, RussiaFederal Research Center of Fundamental and Translational Medicine, Novosibirsk, RussiaFederal Research Center of Fundamental and Translational Medicine, Novosibirsk, RussiaFederal Research Center of Fundamental and Translational Medicine, Novosibirsk, RussiaFederal Research Center of Fundamental and Translational Medicine, Novosibirsk, RussiaFederal Research Center of Fundamental and Translational Medicine, Novosibirsk, RussiaFederal Research Center of Fundamental and Translational Medicine, Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Novosibirsk, RussiaFederal Research Center of Fundamental and Translational Medicine, Novosibirsk, RussiaFederal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia Isolation of human respiratory syncytial virus (HRSV) from clinical samples and storage of isolates for long period remains a considerable problem. We describe in detail the optimized conditions of HRSV isolation and cultivation in three cell cultures HeLa, HEp-2, and Vero. HRSV was detected in 35.2% (166/471) specimens by real-time PCR from symptomatic infants and children up to 15 years from October 2017 to March 2018 in Russia. HRSV-positive samples were used for virus isolation in HeLa, HEp-2, and Vero cells in different manners (in monolayer or suspension). To optimize the conditions of HRSV cultivation, these cell cultures were treated or not with receptor-destroying enzyme (RDE). Ten isolates were successfully obtained by the way of infection of the suspension of cells with subsequent RDE treatment. Among them, several isolates induced the cytopathogenic effect (CPE) by the syncytium formation in both Hela and HEp-2 cell cultures. The genetic analysis revealed that the manners of isolation by using monolayer or suspension and subsequent RDE treatment did not influence the nucleotide and amino acid structures of obtained HRSVs. The CPE characteristics of obtained viruses were the same in HeLa, HEp-2, and Vero cell cultures, and were described as large syncytium up to 150 microns or more in size with the nuclei peripheral location and an optically bright zone in the center of the formation. We showed that infection of cell suspension with the subsequent RDE treatment increased the chance of HRSVs isolation from clinical samples. https://jidc.org/index.php/journal/article/view/17462Human respiratory syncytial virus infectioncultivationisolationsuspensionmonolayersequences |
| spellingShingle | Elena Alexandrovna Prokopyeva Olga Grigoryevna Kurskaya Mariya Vladimirovna Solomatina Ivan Andreevich Sobolev Tereza Araevna Saroyan Nikita Artemyevich Dubovitskiy Anastasiya Alexandrovna Derko Peter Mikchailovich Kozhin Tatyana Yurievna Alikina Marsel Rasimovich Kabilov Alexander Mikchailovich Shestopalov Kirill Alexandrovich Sharshov Virological and genetic characteristics of human respiratory syncytial viruses isolated in Russia, 2017-2018 Journal of Infection in Developing Countries Human respiratory syncytial virus infection cultivation isolation suspension monolayer sequences |
| title | Virological and genetic characteristics of human respiratory syncytial viruses isolated in Russia, 2017-2018 |
| title_full | Virological and genetic characteristics of human respiratory syncytial viruses isolated in Russia, 2017-2018 |
| title_fullStr | Virological and genetic characteristics of human respiratory syncytial viruses isolated in Russia, 2017-2018 |
| title_full_unstemmed | Virological and genetic characteristics of human respiratory syncytial viruses isolated in Russia, 2017-2018 |
| title_short | Virological and genetic characteristics of human respiratory syncytial viruses isolated in Russia, 2017-2018 |
| title_sort | virological and genetic characteristics of human respiratory syncytial viruses isolated in russia 2017 2018 |
| topic | Human respiratory syncytial virus infection cultivation isolation suspension monolayer sequences |
| url | https://jidc.org/index.php/journal/article/view/17462 |
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