Anti-tyrosinase activity of Curcuma aromatica ethyl acetate extract: from lead diarylheptanoids to melanogenesis targets
Abstract Background Curcuma aromatica (C. aromatica), a traditional medicinal plant, holds promise for addressing oxidative stress and hyperpigmentation through its rich curcuminoid and polyphenol content. Building on our previous identification of five major diarylheptanoids in its extract, this st...
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| Format: | Article |
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SpringerOpen
2025-08-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
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| Online Access: | https://doi.org/10.1186/s43088-025-00673-3 |
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| author | Chau Dieu Bui Thuan-Thien Dinh Thang Truong Le Thuan Van Tran Chau Dao Minh Huynh Tho Huu Le Nhan Trung Nguyen Mai Thanh Thi Nguyen Hai Xuan Nguyen Truong Nhat Van Do Hieu Tran-Van |
| author_facet | Chau Dieu Bui Thuan-Thien Dinh Thang Truong Le Thuan Van Tran Chau Dao Minh Huynh Tho Huu Le Nhan Trung Nguyen Mai Thanh Thi Nguyen Hai Xuan Nguyen Truong Nhat Van Do Hieu Tran-Van |
| author_sort | Chau Dieu Bui |
| collection | DOAJ |
| description | Abstract Background Curcuma aromatica (C. aromatica), a traditional medicinal plant, holds promise for addressing oxidative stress and hyperpigmentation through its rich curcuminoid and polyphenol content. Building on our previous identification of five major diarylheptanoids in its extract, this study investigated the ability of C. aromatica to mitigate oxidative stress and modulate melanogenesis with insight into its bioactive compounds’ contributions. Results Spectrophotometric analysis of its ethyl acetate extract revealed significantly high phenolic content, with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays confirming strong antioxidative activity. Using B16 cells, the extract demonstrated non-toxic inhibition of melanin synthesis, reduced tyrosinase activity, and downregulated melanogenic proteins such as tyrosinase, related proteins, and microphthalmia-associated transcription factor (MITF). Gene Ontology and KEGG pathway enrichment analysis (GO-KEGG) along with molecular docking showed that five major diarylheptanoids strongly interacted with these proteins, modulating key pathways involved in pigmentation and beyond. Conclusions Together, these findings highlight C. aromatica’s dual action in combating oxidative stress and inhibiting melanogenesis, positioning it as a promising candidate for advancing medicinal chemistry approaches to hyperpigmentation and oxidative damage. |
| format | Article |
| id | doaj-art-56c9425c02fa4a3aa1f18d86624ff213 |
| institution | Kabale University |
| issn | 2314-8543 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj-art-56c9425c02fa4a3aa1f18d86624ff2132025-08-24T11:45:36ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432025-08-0114111610.1186/s43088-025-00673-3Anti-tyrosinase activity of Curcuma aromatica ethyl acetate extract: from lead diarylheptanoids to melanogenesis targetsChau Dieu Bui0Thuan-Thien Dinh1Thang Truong Le2Thuan Van Tran3Chau Dao Minh Huynh4Tho Huu Le5Nhan Trung Nguyen6Mai Thanh Thi Nguyen7Hai Xuan Nguyen8Truong Nhat Van Do9Hieu Tran-Van10University of ScienceUniversity of ScienceNational Taiwan UniversityUniversity of ScienceUniversity of ScienceUniversity of ScienceUniversity of ScienceUniversity of ScienceUniversity of ScienceUniversity of ScienceUniversity of ScienceAbstract Background Curcuma aromatica (C. aromatica), a traditional medicinal plant, holds promise for addressing oxidative stress and hyperpigmentation through its rich curcuminoid and polyphenol content. Building on our previous identification of five major diarylheptanoids in its extract, this study investigated the ability of C. aromatica to mitigate oxidative stress and modulate melanogenesis with insight into its bioactive compounds’ contributions. Results Spectrophotometric analysis of its ethyl acetate extract revealed significantly high phenolic content, with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays confirming strong antioxidative activity. Using B16 cells, the extract demonstrated non-toxic inhibition of melanin synthesis, reduced tyrosinase activity, and downregulated melanogenic proteins such as tyrosinase, related proteins, and microphthalmia-associated transcription factor (MITF). Gene Ontology and KEGG pathway enrichment analysis (GO-KEGG) along with molecular docking showed that five major diarylheptanoids strongly interacted with these proteins, modulating key pathways involved in pigmentation and beyond. Conclusions Together, these findings highlight C. aromatica’s dual action in combating oxidative stress and inhibiting melanogenesis, positioning it as a promising candidate for advancing medicinal chemistry approaches to hyperpigmentation and oxidative damage.https://doi.org/10.1186/s43088-025-00673-3Curcuma aromaticaAntioxidationMelanogenesis inhibitionTyrosinaseLinear diarylheptanoidsGO-KEGG |
| spellingShingle | Chau Dieu Bui Thuan-Thien Dinh Thang Truong Le Thuan Van Tran Chau Dao Minh Huynh Tho Huu Le Nhan Trung Nguyen Mai Thanh Thi Nguyen Hai Xuan Nguyen Truong Nhat Van Do Hieu Tran-Van Anti-tyrosinase activity of Curcuma aromatica ethyl acetate extract: from lead diarylheptanoids to melanogenesis targets Beni-Suef University Journal of Basic and Applied Sciences Curcuma aromatica Antioxidation Melanogenesis inhibition Tyrosinase Linear diarylheptanoids GO-KEGG |
| title | Anti-tyrosinase activity of Curcuma aromatica ethyl acetate extract: from lead diarylheptanoids to melanogenesis targets |
| title_full | Anti-tyrosinase activity of Curcuma aromatica ethyl acetate extract: from lead diarylheptanoids to melanogenesis targets |
| title_fullStr | Anti-tyrosinase activity of Curcuma aromatica ethyl acetate extract: from lead diarylheptanoids to melanogenesis targets |
| title_full_unstemmed | Anti-tyrosinase activity of Curcuma aromatica ethyl acetate extract: from lead diarylheptanoids to melanogenesis targets |
| title_short | Anti-tyrosinase activity of Curcuma aromatica ethyl acetate extract: from lead diarylheptanoids to melanogenesis targets |
| title_sort | anti tyrosinase activity of curcuma aromatica ethyl acetate extract from lead diarylheptanoids to melanogenesis targets |
| topic | Curcuma aromatica Antioxidation Melanogenesis inhibition Tyrosinase Linear diarylheptanoids GO-KEGG |
| url | https://doi.org/10.1186/s43088-025-00673-3 |
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